scholarly journals Seeing the insulin receptor in action

2018 ◽  
Vol 217 (5) ◽  
pp. 1555-1555
Author(s):  
Ben Short
Keyword(s):  
Electron Microscopy ◽  
Insulin Receptor ◽  
Single Particle ◽  
Receptor Activation ◽  
Single Particle Electron Microscopy

Researchers use single-particle electron microscopy to visualize an insulin-induced conformational shift that leads to receptor activation.

scholarly journals Structure of the full-length VEGFR-1 extracellular domain in complex with VEGF-A

2017 ◽  
Author(s):  
Sandra Markovic-Mueller ◽  
Edward Stuttfeld ◽  
Mayanka Asthana ◽  
Tobias Weinert ◽  
Spencer Bliven ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Tyrosine Kinases ◽  
Extracellular Domain ◽  
Receptor Activation ◽  
Full Length ◽  
Lymph Vessel ◽  
X Ray ◽  
X Ray Crystallography ◽  
Single Particle Electron Microscopy

SUMMARYVascular Endothelial Growth Factors (VEGFs) regulate blood and lymph vessel development upon activation of three receptor tyrosine kinases (RTKs), VEGFR-1, −2, and −3. Partial structures of VEGFR/VEGF complexes based on single particle electron microscopy, small angle X-ray scattering, and X-ray crystallography revealed the location of VEGF binding and domain arrangement of individual receptor subdomains. Here we describe the structure of the full-length VEGFR-1 extracellular domain (ECD) in complex with VEGF-A at 4 Å resolution. We combined X-ray crystallography, single particle electron microscopy, and molecular modeling for structure determination and validation. The structure reveals the molecular details of ligand-induced receptor dimerization, in particular of homotypic receptor interactions in Ig-domains 4, 5, and 7. Functional analyses of ligand binding and receptor activation confirm the relevance of these homotypic contacts and identify them as potential therapeutic sites to allosterically inhibit VEGFR-1 activity.

Modular Imaging Scaffold for Single-Particle Electron Microscopy

ACS Nano ◽  
2021 ◽  
Author(s):  
Nesrine Aissaoui ◽  
Josephine Lai-Kee-Him ◽  
Allan Mills ◽  
Nathalie Declerck ◽  
Zakia Morichaud ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Single Particle Electron Microscopy

scholarly journals Deblurring of class-averaged images in single-particle electron microscopy

2010 ◽  
Vol 26 (3) ◽  
pp. 035002 ◽  
Author(s):  
Wooram Park ◽  
Dean R Madden ◽  
Daniel N Rockmore ◽  
Gregory S Chirikjian
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Single Particle Electron Microscopy

scholarly journals Single particle electron microscopy

2009 ◽  
Vol 102 (2-3) ◽  
pp. 189-196 ◽  
Author(s):  
Egbert J. Boekema ◽  
Mihaela Folea ◽  
Roman Kouřil
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Single Particle Electron Microscopy

scholarly journals Projection Structure by Single-Particle Electron Microscopy of Secondary Transport Proteins GltT, CitS, and GltS

Biochemistry ◽  
2009 ◽  
Vol 48 (28) ◽  
pp. 6618-6623 ◽  
Author(s):  
Katarzyna B. Mościcka ◽  
Tomasz Krupnik ◽  
Egbert J. Boekema ◽  
Juke S. Lolkema
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Transport Proteins ◽  
Secondary Transport ◽  
Single Particle Electron Microscopy

Angle determination for side views in single particle electron microscopy

2008 ◽  
Vol 162 (2) ◽  
pp. 260-270 ◽  
Author(s):  
Lindsay A. Baker ◽  
John L. Rubinstein
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Single Particle Electron Microscopy
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