DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment.
1976 ◽
Vol 69
(3)
◽
pp. 732-736
◽
We have developed a method for permeabilizing CHO cells to nucleotides under conditions which allow most cells to remain viable. Permeabilized cells can carry out ATP-dependent, semiconservative synthesis of DNA. The data are consistent with the continuation of DNA synthesis in those cells in S phase at the time of treatment, possibly limited to completion of replicon synthesis without new initiations.