scholarly journals THE UROPOD-BEARING LYMPHOCYTE OF THE GUINEA PIG

1972 ◽  
Vol 135 (5) ◽  
pp. 1037-1048 ◽  
Author(s):  
David L. Rosenstreich ◽  
Ethan Shevach ◽  
Ira Green ◽  
Alan S. Rosenthal
Keyword(s):  
Lymph Node ◽  
Guinea Pig ◽  
Human Lymphocytes ◽  
Surface Membrane ◽  
Purified Protein Derivative ◽  
Membrane Immunoglobulin ◽  
Lymph Node Cells ◽  
Lymphocyte Populations ◽  
Antigen Reactivity

In this study, the frequency of uropod formation and the type of lymphocyte bearing the uropod was investigated in various guinea pig lymphocyte populations. Without prior in vitro stimulation, almost 40% of peritoneal exudate lymphocytes (PELS) form uropods, while thymocytes and lymph node cells form far fewer. Subsequent stimulation in vitro with purified protein derivative demonstrated that there is an association between antigen reactivity and frequency of uropod formation in these populations. The ultrastructure of these uropods is identical to that described for human lymphocytes stimulated with phytohemagglutinin. In the populations studied, all the lymphocytes forming uropods lack easily detectable surface membrane immunoglobulin and are therefore most likely thymus-derived or T lymphocytes.

scholarly journals BINDING OF AGGREGATED γ-GLOBULIN TO ACTIVATED T LYMPHOCYTES IN THE GUINEA PIG

1974 ◽  
Vol 139 (4) ◽  
pp. 1002-1012 ◽  
Author(s):  
John A. van Boxel ◽  
David L. Rosenstreich
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
T Lymphocytes ◽  
Guinea Pig ◽  
Surface Membrane ◽  
Cell Populations ◽  
Cell Marker ◽  
Activated T Cells ◽  
Activated T Lymphocytes

Heat-aggregated guinea pig γ-globulin was shown to bind to the surface membrane of a subclass of guinea pig T lymphocytes. Cells of this subpopulation were identified as T lymphocytes because these cells did not stain for surface Ig (a B-cell marker) but did form spontaneous E-rosettes with rabbit erythrocytes (a T-cell marker). A strikingly high proportion of such aggregate-binding (Agg+), E-rosette-forming (E-rosette+), but surface Ig-negative (Ig-) cells were found in an inflammatory exudate. Thus purified peritoneal exudate lymphocytes (PELs) are known to consist of over 90% T cells, and 59% of these cells bound aggregates. 10% of these Agg+ Ig- E-rosette+ cells were found in draining lymph node cell populations and none in thymus cell populations. The high frequency amongst PELs suggested that these Aggregate+ Ig- E-rosette+ cells might be activated T cells as these are known to occur in high proportion in PEL populations. Confirmatory evidence for this postulate was provided by the striking increase (from 10% to 46%) of Ig- E-rosette+ cells that bound aggregates when lymph node cells were activated by antigen stimulation in vitro.

scholarly journals A QUANTITATIVE STUDY OF THE STIMULATION OF DNA SYNTHESIS IN LYMPH NODE CELL CULTURES BY ANTI-LYMPHOCYTE SERUM, ANTI-GAMMA GLOBULIN SERUM, SPECIFIC ANTIGEN, AND PHYTOHEMAGGLUTININ

1969 ◽  
Vol 129 (2) ◽  
pp. 295-313 ◽  
Author(s):  
John Foerster ◽  
Jean-Pierre Lamelin ◽  
Ira Green ◽  
Baruj Benacerraf
Keyword(s):  
Lymph Node ◽  
Guinea Pig ◽  
Gamma Globulin ◽  
Narrow Range ◽  
Specific Antigen ◽  
Lymph Node Cell ◽  
Wide Range ◽  
Lymph Node Cells ◽  
Stimulation Of

Rabbit anti-guinea pig lymphocyte serum is an efficient stimulus of the synthesis of DNA by guinea pig lymph node cells in vitro. The ability of ALS to stimulate lymphocytes is characterized by its lack of dependence on prior sensitization, the magnitude of the response it elicits, and the stimulation of all sensitive lymph node cells simultaneously within a very narrow range of ALS concentrations. In contrast to this homogeneous response to ALS, the stimulation of lymph node cells by antigen proceeds in graded fashion over a wide range of concentrations, thus reflecting the heterogeneity of the response of sensitized cells to antigen. PHA gives a response which is intermediate between that of ALS and antigen. ALS appears to have specificity for membrane determinants shared by lymphocytes but not found on other tissues. This specificity does not involve cell-bound gamma globulin. The serum activity mediating lymphocyte stimulation as well as cytotoxicity is readily removed by absorption with lymph node cells.

scholarly journals Congenitally athymic nude (nu/nu) mice have Thy-1-bearing immunocompetent helper T cells in their peritoneal cavity.

1980 ◽  
Vol 151 (4) ◽  
pp. 965-968 ◽  
Author(s):  
H Ishikawa ◽  
K Saito
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
Guinea Pig ◽  
Antibody Response ◽  
Sheep Erythrocyte ◽  
Helper T Cells ◽  
Spleen Cells ◽  
Lymph Node Cells ◽  
Pig Serum

Heavily irradiated peritoneal cells (PC) from congenitally athymic nude (nu/nu) mice markedly restored the impaired in vitro antibody response of nu/nu spleen cells to sheep erythrocyte antigens (T-dependent antigen), whereas irradiated spleen or lymph node cells from nu/nu mice had no effect on the response. This activity of the irradiated PC of nu/nu mice was completely abolished by treatment with anti-Thy-1.2 antiserum plus normal guinea pig serum (C') and is, therefore, attributable to a function of matured T cells.

scholarly journals AUTOSENSITIZATION REACTION IN VITRO

1962 ◽  
Vol 116 (4) ◽  
pp. 467-476 ◽  
Author(s):  
Hilary Koprowski ◽  
Mario V. Fernandes
Keyword(s):  
Tissue Culture ◽  
Lymph Node ◽  
Guinea Pig ◽  
Inbred Strain ◽  
Brain Tissue ◽  
Glial Cells ◽  
Agglutination Test ◽  
Lymph Node Cells ◽  
Brain Tissue Culture

Lymph node cells were obtained from an inbred strain of Lewis rats injected with guinea pig cord tissue in Freund's adjuvant. These cells, when added to tissue culture monolayers of puppy brain, aggregated on or around the glial elements. This reaction, called contactual agglutination, was followed by the specific destruction of glial cells, leaving cultures consisting only of fibroblasts. No such reaction was noted when lymph node cells obtained either from normal rats or those injected with adjuvant alone were used. Absorption of serum obtained from rats injected with guinea pig cord tissue by non-sensitized lymph node cells made them reactive in brain tissue culture. The contactual agglutination test seems to provide an opportunity for investigation of sensitization reaction in tissue culture systems.

scholarly journals Transfer of experimental allergic encephalomyelitis in Lewis rats using supernates of incubated sensitized lymph node cells.

1977 ◽  
Vol 145 (5) ◽  
pp. 1405-1410 ◽  
Author(s):  
C C Whitacre ◽  
P Y Paterson
Keyword(s):  
Spinal Cord ◽  
Lymph Node ◽  
Guinea Pig ◽  
Experimental Allergic Encephalomyelitis ◽  
Nervous Tissue ◽  
Allergic Encephalomyelitis ◽  
Lewis Rats ◽  
Transfer Activity ◽  
Lymph Node Cells ◽  
Experimental Allergic

Supernates derived from incubated lymph node cells of Lewis rats sensitized to guinea pig spinal cord-Freund's adjuvant transfer experimental allergic encephalomyelitis (EAE) to syngeneic recipients. EAE supernatant transfer activity (EAE-STA) is not demonstrable in supernates derived from LNC of control donors not sensitized to nervous tissue. After addition of brain antigen to active supernates, EAE-STA is not longer demonstrable.

Action of Titanium on Guinea Pig Macrophages and Human Lymphocytes Cultured in Vitro.

1974 ◽  
Vol 60 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Roberto Scelsi ◽  
Enrico Cislaghi ◽  
Mario Scelsi
Keyword(s):  
Guinea Pig ◽  
Untreated Control ◽  
Human Lymphocytes ◽  
Negative Influence ◽  
Blast Transformation

The action of titanium on guinea pig macrophages and human lymphocytes cultured in vitro is studied with the fluorochromasia test and with the extent of blast transformation and mitoses. No differences between the percentages of macrophages which show fluorochromasia are observed in cultures with titanium and in untreated control cultures. Titanium doesn't exert negative influence on reduplication of human lymphocytes cultured in vitro with phytohemoagglutinine (P.H.A.).

The Activation and Reactivation of Peripheral Lymphocytes in Culture

1967 ◽  
Vol 2 (1) ◽  
pp. 57-70
Author(s):  
N. R. LING ◽  
P. J. L. HOLT
Keyword(s):  
Mixed Lymphocyte Reaction ◽  
Human Lymphocytes ◽  
Maximal Activity ◽  
Cell Populations ◽  
Initial Stimulus ◽  
Purified Protein Derivative ◽  
Peripheral Lymphocytes ◽  
Lymphocyte Populations ◽  
Low Dosage

Human lymphocytes stimulated for 16 h and then cultured without stimulant showed maximal activity on days 2-3 following a stimulus of phytohaemagglutinin (PHA) and on days 3-4 following a stimulus of staphylococcal filtrate (SF). At low dosage of stimulant the response of the cells was less marked but persisted for a longer period than at high dosage. The pattern of response is discussed in relation to the mechanism of activation. After the effect of the initial stimulus had died away cell populations which had been stimulated with SF or PHA could be restimulated with either stimulant. Their response, when stimulated this second time, was quicker than that of incubated cells from the same donor which had not been previously stimulated. Prestimulated cells were also tested in two immunospecific reactions : the reaction to tuberculin-purified protein derivative (PPD), and the mixed lymphocyte reaction. Cells which had been previously exposed to SF responded more quickly to PPD than cells not previously stimulated. Cell populations which had been previously stimulated also reacted more quickly in a mixed lymphocyte reaction. It is concluded that lymphocyte populations which have been recently stimulated not only retain their capacity to react to immunospecific mitotic stimuli but also react more quickly.

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