scholarly journals AUTOSENSITIZATION REACTION IN VITRO

1962 ◽  
Vol 116 (4) ◽  
pp. 467-476 ◽  
Author(s):  
Hilary Koprowski ◽  
Mario V. Fernandes
Keyword(s):  
Tissue Culture ◽  
Lymph Node ◽  
Guinea Pig ◽  
Inbred Strain ◽  
Brain Tissue ◽  
Glial Cells ◽  
Agglutination Test ◽  
Lymph Node Cells ◽  
Brain Tissue Culture

Lymph node cells were obtained from an inbred strain of Lewis rats injected with guinea pig cord tissue in Freund's adjuvant. These cells, when added to tissue culture monolayers of puppy brain, aggregated on or around the glial elements. This reaction, called contactual agglutination, was followed by the specific destruction of glial cells, leaving cultures consisting only of fibroblasts. No such reaction was noted when lymph node cells obtained either from normal rats or those injected with adjuvant alone were used. Absorption of serum obtained from rats injected with guinea pig cord tissue by non-sensitized lymph node cells made them reactive in brain tissue culture. The contactual agglutination test seems to provide an opportunity for investigation of sensitization reaction in tissue culture systems.

scholarly journals THE UROPOD-BEARING LYMPHOCYTE OF THE GUINEA PIG

1972 ◽  
Vol 135 (5) ◽  
pp. 1037-1048 ◽  
Author(s):  
David L. Rosenstreich ◽  
Ethan Shevach ◽  
Ira Green ◽  
Alan S. Rosenthal
Keyword(s):  
Lymph Node ◽  
Guinea Pig ◽  
Human Lymphocytes ◽  
Surface Membrane ◽  
Purified Protein Derivative ◽  
Membrane Immunoglobulin ◽  
Lymph Node Cells ◽  
Lymphocyte Populations ◽  
Antigen Reactivity

In this study, the frequency of uropod formation and the type of lymphocyte bearing the uropod was investigated in various guinea pig lymphocyte populations. Without prior in vitro stimulation, almost 40% of peritoneal exudate lymphocytes (PELS) form uropods, while thymocytes and lymph node cells form far fewer. Subsequent stimulation in vitro with purified protein derivative demonstrated that there is an association between antigen reactivity and frequency of uropod formation in these populations. The ultrastructure of these uropods is identical to that described for human lymphocytes stimulated with phytohemagglutinin. In the populations studied, all the lymphocytes forming uropods lack easily detectable surface membrane immunoglobulin and are therefore most likely thymus-derived or T lymphocytes.

scholarly journals A QUANTITATIVE STUDY OF THE STIMULATION OF DNA SYNTHESIS IN LYMPH NODE CELL CULTURES BY ANTI-LYMPHOCYTE SERUM, ANTI-GAMMA GLOBULIN SERUM, SPECIFIC ANTIGEN, AND PHYTOHEMAGGLUTININ

1969 ◽  
Vol 129 (2) ◽  
pp. 295-313 ◽  
Author(s):  
John Foerster ◽  
Jean-Pierre Lamelin ◽  
Ira Green ◽  
Baruj Benacerraf
Keyword(s):  
Lymph Node ◽  
Guinea Pig ◽  
Gamma Globulin ◽  
Narrow Range ◽  
Specific Antigen ◽  
Lymph Node Cell ◽  
Wide Range ◽  
Lymph Node Cells ◽  
Stimulation Of

Rabbit anti-guinea pig lymphocyte serum is an efficient stimulus of the synthesis of DNA by guinea pig lymph node cells in vitro. The ability of ALS to stimulate lymphocytes is characterized by its lack of dependence on prior sensitization, the magnitude of the response it elicits, and the stimulation of all sensitive lymph node cells simultaneously within a very narrow range of ALS concentrations. In contrast to this homogeneous response to ALS, the stimulation of lymph node cells by antigen proceeds in graded fashion over a wide range of concentrations, thus reflecting the heterogeneity of the response of sensitized cells to antigen. PHA gives a response which is intermediate between that of ALS and antigen. ALS appears to have specificity for membrane determinants shared by lymphocytes but not found on other tissues. This specificity does not involve cell-bound gamma globulin. The serum activity mediating lymphocyte stimulation as well as cytotoxicity is readily removed by absorption with lymph node cells.

scholarly journals Congenitally athymic nude (nu/nu) mice have Thy-1-bearing immunocompetent helper T cells in their peritoneal cavity.

1980 ◽  
Vol 151 (4) ◽  
pp. 965-968 ◽  
Author(s):  
H Ishikawa ◽  
K Saito
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
Guinea Pig ◽  
Antibody Response ◽  
Sheep Erythrocyte ◽  
Helper T Cells ◽  
Spleen Cells ◽  
Lymph Node Cells ◽  
Pig Serum

Heavily irradiated peritoneal cells (PC) from congenitally athymic nude (nu/nu) mice markedly restored the impaired in vitro antibody response of nu/nu spleen cells to sheep erythrocyte antigens (T-dependent antigen), whereas irradiated spleen or lymph node cells from nu/nu mice had no effect on the response. This activity of the irradiated PC of nu/nu mice was completely abolished by treatment with anti-Thy-1.2 antiserum plus normal guinea pig serum (C') and is, therefore, attributable to a function of matured T cells.

In Vitro Formation of Eosinophil-Like Colonies by Lymph Node Cells from a Human Lymphoma

1980 ◽  
Vol 66 (2) ◽  
pp. 153-158
Author(s):  
Luigi P. Ruco ◽  
Stefania Uccini ◽  
Patrizia Rigato ◽  
Carlo D. Baroni
Keyword(s):  
Tissue Culture ◽  
Lymph Node ◽  
Giant Cells ◽  
Multinucleated Giant Cells ◽  
Ring Form ◽  
Non Hodgkin Lymphoma ◽  
Human Lymphoma ◽  
Lymph Node Cells ◽  
Eosinophil Colonies

In vitro culturing of lymph node cells from a human non-Hodgkin lymphoma gave rise to several colonies of eosinophil-like cells. Eosinophil colonies originated from cells that during the first week of culture had a fibroblast appearance and were adherent to plastic. The tissue culture was sacrificed after 14 days. At that time each colony was formed by 20–50 cells with intracytoplasmic peroxidase-positive and eosinophilic granules. Cells comprising the colonies exhibited different degrees of differentiation. Some of the cells (26.6 %) were mature eosinophils, the majority (66.8 %) resembled eosinophil myelocytes, and some others (4.6 %) had a fibroblast appearance. One or two multinucleated giant cells were often present in the center of most of the colonies. These cells contained up to 10 nuclei, which were arranged in a «ring form» or centrally located; giant cells with a single, central, large, multilobed nucleus were also observed. Cells belonging to other myelopoietic lines could not be identified in the tissue culture. Histological examination of the lymph node revealed extensive presence of eosinophils at various degrees of maturation but absence of other myelopoietic lines.

scholarly journals Transfer of experimental allergic encephalomyelitis in Lewis rats using supernates of incubated sensitized lymph node cells.

1977 ◽  
Vol 145 (5) ◽  
pp. 1405-1410 ◽  
Author(s):  
C C Whitacre ◽  
P Y Paterson
Keyword(s):  
Spinal Cord ◽  
Lymph Node ◽  
Guinea Pig ◽  
Experimental Allergic Encephalomyelitis ◽  
Nervous Tissue ◽  
Allergic Encephalomyelitis ◽  
Lewis Rats ◽  
Transfer Activity ◽  
Lymph Node Cells ◽  
Experimental Allergic

Supernates derived from incubated lymph node cells of Lewis rats sensitized to guinea pig spinal cord-Freund's adjuvant transfer experimental allergic encephalomyelitis (EAE) to syngeneic recipients. EAE supernatant transfer activity (EAE-STA) is not demonstrable in supernates derived from LNC of control donors not sensitized to nervous tissue. After addition of brain antigen to active supernates, EAE-STA is not longer demonstrable.

Understanding the Physiology of the Blood-Brain Barrier: In Vitro Models

Physiology ◽  
1998 ◽  
Vol 13 (6) ◽  
pp. 287-293 ◽  
Author(s):  
Gerald A. Grant ◽  
N. Joan Abbott ◽  
Damir Janigro
Keyword(s):  
Central Nervous System ◽  
Tissue Culture ◽  
Nervous System ◽  
Blood Brain Barrier ◽  
In Vitro Models ◽  
Brain Barrier ◽  
Blood Brain ◽  
Brain Tissue Culture ◽  
The Brain

Endothelial cells exposed to inductive central nervous system factors differentiate into a blood-brain barrier phenotype. The blood-brain barrier frequently obstructs the passage of chemotherapeutics into the brain. Tissue culture systems have been developed to reproduce key properties of the intact blood-brain barrier and to allow for testing of mechanisms of transendothelial drug permeation.

Slow spontaneous signals from brain tissue culture

1970 ◽  
Vol 26 (1) ◽  
pp. 13-16 ◽  
Author(s):  
A. W. B. Cunningham ◽  
A. E. Hamilton ◽  
M. F. King ◽  
R. R. Rojas-Corona ◽  
G. F. Songster
Keyword(s):  
Tissue Culture ◽  
Brain Tissue ◽  
Brain Tissue Culture
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