scholarly journals A STUDY OF ALLOGRAFT KIDNEY REJECTION OCCURRING SIMULTANEOUSLY IN WHOLE ORGAN AND EAR CHAMBER GRAFTS IN THE RABBIT

1973 ◽  
Vol 137 (3) ◽  
pp. 776-798 ◽  
Author(s):  
J. B. Hobbs ◽  
W. J. Cliff
Keyword(s):  
Thrombus Formation ◽  
Renal Tissue ◽  
High Power Field ◽  
Allograft Kidney ◽  
Rabbit Ear ◽  
Rabbit Ear Chamber ◽  
Kidney Rejection ◽  
Adherent Leukocytes ◽  
Parenchymal Damage

When portions of adult renal tissue are allografted into the rabbit ear chamber, they usually survive for periods of up to several months (6). When a kidney from the same donor is grafted as a whole organ, the ear chamber grafts then reject with the whole organ in 7 days. During that time serial needle biopsies of the whole organ are compared with the in vivo appearance of the ear chamber grafts. This establishes that the changes occurring in the ear chamber grafts are monitoring the rejection process proceeding in the whole organ grafts. Dramatic vascular changes herald the earliest stages of unmodified rejection. A highly characteristic form of individual discrete platelet adhesion to both endothelium and adherent leukocytes is observed which is associated with the release reaction. At times as many as 20 such discrete platelets are clearly visible in profile in one high-power field. This demonstrates in vivo a mechanism whereby vascular and parenchymal damage may be produced by platelet contents, without previous aggregation or thrombus formation being necessary.

An Electron Microscopic Study of Platelet Thrombus Formation in the Rabbit with Particular Regard to 5-Hydroxytryptamine Release

1967 ◽  
Vol 18 (03/04) ◽  
pp. 592-604 ◽  
Author(s):  
H. R Baumgartner ◽  
J. P Tranzer ◽  
A Studer
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Vessel Wall ◽  
Thrombus Formation ◽  
Endothelial Damage ◽  
Electron Microscopic ◽  
Rabbit Ear ◽  
Basement Lamina ◽  
Platelet Thrombus

SummaryElectron microscopic and histologic examination of rabbit ear vein segments 4 and 30 min after slight endothelial damage have yielded the following findings :1. Platelets do not adhere to damaged endothelial cells.2. If the vessel wall is denuded of the whole endothelial cell, platelets adhere to the intimai basement lamina as do endothelial cells.3. The distance between adherent platelets as well as endothelial cells and intimai basement lamina measures 10 to 20 mµ, whereas the distance between aggregated platelets is 30 to 60 mµ.4. 5-hydroxytryptamine (5-HT) is released from platelets during viscous metamorphosis at least in part as 5-HT organelles.It should be noted that the presence of collagen fibers is not necessary for platelet thrombus formation in vivo.

scholarly journals THE ACUTE INFLAMMATORY REACTION IN THE RABBIT EAR CHAMBER WITH PARTICULAR REFERENCE TO THE PHENOMENON OF LEUKOCYTIC MIGRATION

1966 ◽  
Vol 124 (4) ◽  
pp. 543-556 ◽  
Author(s):  
W. J. Cliff
Keyword(s):  
Inflammatory Responses ◽  
Specific Activity ◽  
Time Lapse ◽  
Amoeboid Movement ◽  
Inflammatory Reactions ◽  
Rabbit Ear ◽  
Rabbit Ear Chamber ◽  
Venular Endothelium

Responses to injections of various materials into rabbit ear chambers were studied by in vivo microscopy. The acute inflammatory responses provoked by injections of antibody-antigen complexes were both quantitatively and qualitatively different from the responses obtained after injections of either homologous sera or the antigens alone. The sticking of leukocytes to endothelium during these responses occurred only in the venules draining the injection sites and was frequently present only on the sides of the venules towards the injection sites. An explanation of this finding was proposed in terms of absorption by the minute vessels related to the injection sites of postulated mediator(s) with specific activity on venular endothelium. Analysis of the rates and direction of movement of leukocytes during the reactions produced by the antibody-antigen complexes was performed with the aid of time-lapse cinemicroscopy. The leukocytes that were sticking to the venular endothelium frequently exhibited amoeboid locomotion within the vessels. Twice as many of these cells moved against the direction of blood flow as with it. This finding was discussed and an explanation proposed. A method for detecting a drift in the overall population of emigrated leukocytes within the inflamed tissue was described and revealed that four times as many amoeboid cells moved away from the injection sites as towards them. This result was discussed in the light of the in vitro chemotactic properties of antibody-antigen complexes demonstrated for rabbit leukocytes. An alternative explanation was proposed in terms of variation in the population density of these cells and their random movements and collisions. The rates of amoeboid movement of leukocytes during the acute inflammatory reactions produced by antibody-antigen complexes were similar to the rates found during turpentine inflammation and were compared to other published values.

The Effect of Serotonin Inhibition on Initial Microvessel Hemostasis and Platelet Aggregation In Vivo

1983 ◽  
Vol 49 (03) ◽  
pp. 173-175 ◽  
Author(s):  
D Bergqvist ◽  
S Arvidsson ◽  
C O Esquivel ◽  
B Lindblad ◽  
U Haglund
Keyword(s):  
Adrenergic Receptor ◽  
Formation Time ◽  
Platelet Activity ◽  
Receptor Affinity ◽  
Rabbit Ear ◽  
Rabbit Ear Chamber ◽  
Plug Formation ◽  
Mesenteric Microcirculation

SummaryThe role of serotonin (5-HT) in initial microvascular hemostasis is not fully understood. This study was made to evaluate the effect on hemostatic plug formation and laser-induced arteriolar microembolism of different substances which counteract the effect of 5-HT. Hemostatic plug formation time and stability was measured in the rabbit mesenteric microcirculation and laserinduced embolism in the rabbit ear chamber. Ketanserine, a selective 5-HT2-receptor blocker shortened arteriolar hemostatic plug formation time. Dihydroergotamine, an unselective blocker (with 5-HT- and α-adrenergic receptor affinity) increased venular hemostatic plug formation time and also decreased the hemostatic plug stability. Laser-induced platelet embolism was unaltered after both ketanserine and dihydroergotamine administration. The magnitude of these changes seems to exclude an important effect of 5-HT in initial microvessel hemostasis or on platelet activity.

Radiation Damage and Subendothelial Repair to Rabbit Ear Chamber Microvasculature: An in Vivo and Histologic Study

1977 ◽  
Vol 69 (2) ◽  
pp. 276 ◽  
Author(s):  
G. S. Dimitrievich ◽  
S. L. Hausladen ◽  
F. T. Kuchnir ◽  
M. L. Griem
Keyword(s):  
Radiation Damage ◽  
Histologic Study ◽  
Rabbit Ear ◽  
Rabbit Ear Chamber
Sign in / Sign up

Export Citation Format

Share Document