scholarly journals Suppressor cells in tolerance to contact sensitivity active against hapten-syngeneic and hapten-allogeneic determinants.

1977 ◽  
Vol 146 (1) ◽  
pp. 49-58 ◽  
Author(s):  
H N Claman ◽  
S D Miller ◽  
M S Sy
Keyword(s):  
T Cell ◽  
T Cell Receptors ◽  
Suppressor Cells ◽  
Mouse Spleen ◽  
Gene Products ◽  
Contact Sensitivity ◽  
Spleen Cells ◽  
Apparent Absence ◽  
Cell Receptors ◽  
Vh Gene

Genetic restrictions in generation and expression of hapten-specific suppressor cells for contact sensitivity were found. Dinitrophenol- (DNP) or trinitrophenol-modified mouse spleen cells (SC) induced suppressors in donors able to transfer suppression to normal recipients. When allogeneic DNP-SC were injected into BALB/c mice, cells were generated which were suppressive only in the allogeneic strain providing the DNP-SC. In contrast, when DNP-BALB/c-SC were injected into BALB/c mice, suppressors were generated which were active both in BALB/c and in allogeneic mice (e.g., CBA). This apparent absence of syngeneic major histocompability complex restriction may be explained by cross reactive T-cell receptors which are VH gene products.

Two Different VH Gene Products Make Up the T-Cell Receptors

1976 ◽  
Vol 5 (9) ◽  
pp. 993-1001 ◽  
Author(s):  
C. A. Janeway ◽  
H. Wigzell ◽  
H. Binz
Keyword(s):  
T Cell ◽  
T Cell Receptors ◽  
Gene Products ◽  
Cell Receptors ◽  
Vh Gene

scholarly journals Mechanisms of regulation of cell-mediated immunity. III. The characterization of azobenzenearsonate-specific suppressor T-cell-derived-suppressor factors.

1979 ◽  
Vol 149 (5) ◽  
pp. 1069-1083 ◽  
Author(s):  
M I Greene ◽  
B A Bach ◽  
B Benacerraf
Keyword(s):  
T Cell ◽  
Suppressor Cells ◽  
Binding Specificity ◽  
Delayed Type Hypersensitivity ◽  
Antigen Binding ◽  
Gene Products ◽  
Suppressive Activity ◽  
Spleen Cells ◽  
Suppressor Factor ◽  
Suppressor T Cell

Delayed type hypersensitivity to the hapten azobenzenearsonate (ABA) can be induced and suppressed by the administration of hapten-coupled syngeneic spleen cells by the appropriate route. Suppressor T cells stimulated by the intravenous administration of ABA-coupled spleen cells have been shown to produce a discrete subcellular factor(s) which is capable of suppressing delayed type hypersensitivity to azobenzenearsonate in the mouse. Such suppressor factors may be produced by the mechanical disruption of suppressor cells or by placing such suppressor cells in culture for 24 h. The suppressor factor(s) (SF) derived from ABA-specific suppressor cells exhibit biological specificity for the suppression of ABA delayed type hypersensitivity (DTH), but not trinitro-phenyl DTH, as well as the capacity to bind to ABA immunoadsorbents. Passage of suppressor factor(s) over reverse immunoadsorbents utilizing a rabbit anti-mouse F(ab')2 antiserum demonstrated that the antigen-specific T-cell derived SF does not bear conventional immunoglobulin markers. The suppressor factor(s) are not immunoglobulin molecules was further demonstrated by the inability of anti-ABA antibodies to suppress ABA DTH. Gel filtration of ABA suppressor factor(s) showed that the majority of the suppressive activity was present in a fraction with molecular weight ranging between 6.8 x 10(4) and 3.3 x 10(4) daltons. We also analyzed for the presence of determinants encoded by the H-2 major histocompatibility complex (MHC) and found that immunoadsorbents prepared utilizing antisera capable of interacting with gene products of the whole or selected gene regions of H-2 MHC, i.e., B10.D2 anti-B10.A and B10 anti-B10.A immunoadsorbents, retained the suppressive activity of ABA-SF. Elution of such columns with glycine HCl buffers (pH 2.8) permitted recovery of specific suppressive activity. Taken collectively such data supports the notion that suppressor T-cell-derived ABA suppressor factors have antigen-binding specificity as well as determinants controlled by the K end of the H-2 MHC. The distribution of strains capable of making SF has also been analyzed. The relationship of the antigen-binding specificity to VH gene products is discussed in this and the companion paper.

Dominance of the BV17 element in nickel-specific human T cell receptors relates to severity of contact sensitivity

1997 ◽  
Vol 27 (8) ◽  
pp. 1865-1874 ◽  
Author(s):  
Jörg Vollmer ◽  
Michaela Fritz ◽  
Anne Dormoy ◽  
Hans Ulrich Weltzien ◽  
Corinne Moulon
Keyword(s):  
T Cell ◽  
T Cell Receptors ◽  
Contact Sensitivity ◽  
Cell Receptors ◽  
Human T Cell

Dominance of the AV1 and BV17 elements in nickel-specific human T cell receptors relates to severity of contact sensitivity

1997 ◽  
Vol 56 ◽  
pp. 347
Author(s):  
C. Moulon ◽  
J. Vollmer ◽  
M. Fritz ◽  
A. Dormoy ◽  
H.U. Weltzien
Keyword(s):  
T Cell ◽  
T Cell Receptors ◽  
Contact Sensitivity ◽  
Cell Receptors ◽  
Human T Cell

T-cell receptors of human suppressor cells

Nature ◽  
1987 ◽  
Vol 329 (6139) ◽  
pp. 541-545 ◽  
Author(s):  
Robert L. Modlin ◽  
Michael B. Brenner ◽  
Michael S. Krangel ◽  
Allan D. Duby ◽  
Barry R. Bloom
Keyword(s):  
T Cell ◽  
T Cell Receptors ◽  
Suppressor Cells ◽  
Cell Receptors

scholarly journals H-2-restricted helper factor secreted by clone hybridoma cells.

1981 ◽  
Vol 154 (3) ◽  
pp. 942-951 ◽  
Author(s):  
P Lonai ◽  
J Puri ◽  
S Bitton ◽  
Y Ben-Neriah ◽  
D Givol ◽  
...  
Keyword(s):  
T Cell ◽  
B Cell ◽  
T Cell Receptors ◽  
Hybridoma Cells ◽  
Spleen Cells ◽  
Specific Antibodies ◽  
Cell Receptors ◽  
Helper Cells ◽  
Cell Sources ◽  
Helper Factor

Biological and serological characteristics of a helper factor secreted by cloned hybridoma cells was described. The factor is carrier specific and contains determinants shared with immunoglobulin VH bu does not react with V kappa- or V lambda-specific antibodies. Presence of four H-2I-controlled antigenic specificities, Ia.ml, Ia.m2, Ia.17, and Ia.m7, was detected. Hence, it is possible that both A beta and E alpha loci may be involved in its control. Helper effect could be obtained only toward B cell sources that shared the H-2K and I-A antigens with the hybridoma cells. Similarly, the factor was absorbed only by spleen cells syngeneic in I-A. Previous studies have demonstrated that this clone binds antigen in an H-2-restricted manner. It follows that H-2-restricted helper cells produce H-2-restricted helper factors. Hence, they support the view that specific T cell factors may represent secreted T cell receptors.

scholarly journals SPONTANEOUS RELEASE OF T-CELL RECEPTORS FOR ALLOANTIGENS

1974 ◽  
Vol 140 (3) ◽  
pp. 603-618 ◽  
Author(s):  
Hansruedy Ramseier
Keyword(s):  
T Cell ◽  
T Cell Receptors ◽  
Bone Marrow Cells ◽  
Culture Conditions ◽  
In Vitro Cultivation ◽  
Spontaneous Release ◽  
Spleen Cells ◽  
Cell Receptors ◽  
Murine Spleen

In vitro cultivation of murine spleen cells resulted in a spontaneous release of receptors for alloantigens. This was revealed by the capacity of cell-free culture supernates to recognize alloantigens as measured in the PAR assay. Qualitatively, recognition responses obtained with these supernates reproduced faithfully those found with the corresponding cells. Large amounts of receptors were released by untreated spleen cells and by spleen cells treated with a rabbit antimouse immunoglobulin serum and complement, smaller amounts were released by bone marrow cells, and native thymus cells released none. Spleen cells from nude mice and spleen cells from normal mice treated with anti-θ serum and complement showed no release of receptors. It was concluded that receptors active in the PAR test were of T-cell origin. Release of T-cell receptors was found to be discontinuous and proceeded in waves. The amount of released receptors depended on the number of cells cultivated. Release occurred at 37°C but not at 4°C. Interaction with antigen, however, was temperature-independent. In contrast to T-cell receptors, a release of H-2 antigens could not be detected with the culture conditions employed.

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