scholarly journals Modulation of HLA class II antigen expression by transfection of sense and antisense DR alpha cDNA.

1989 ◽  
Vol 169 (1) ◽  
pp. 351-356 ◽  
Author(s):  
V Lotteau ◽  
J Sands ◽  
L Teyton ◽  
P Turmel ◽  
D Charron ◽  
...  
Keyword(s):  
Mhc Class Ii ◽  
Antigen Expression ◽  
Class Ii ◽  
High Ratio ◽  
Hla Class Ii ◽  
Gene Products ◽  
Beta Chain ◽  
Hla Dr ◽  
Alpha Beta ◽  
Class Ii Antigen

In the human there are three isotypic forms of MHC class II gene products (HLA-DR, -DQ, and -DP). The isotype-matched alpha-beta dimers are predominant but isotype-mismatched dimers can also be expressed (DR alpha-DQ beta). Here it is shown that the expression of the DR alpha-DQ beta dimer can be correlated to a high ratio of DR alpha/DR beta mRNA. The DR alpha chain expression was modulated by transfection of a sense and antisense DR alpha cDNA. Overexpression of DR alpha promoted the appearance of the DR alpha-DQ beta dimer. On the other hand, pre-existing DR alpha-DQ beta dimer expression was suppressed after antisense DR alpha cDNA transfection. Therefore, imbalanced expression of the alpha and beta chain from a given isotype could lead to the modification of HLA class II phenotype.

scholarly journals Supernatants of human leukocytes contain mediator, different from interferon gamma, which induces expression of MHC class II antigens.

1986 ◽  
Vol 164 (1) ◽  
pp. 131-143 ◽  
Author(s):  
G Groenewegen ◽  
M de Ley ◽  
G M Jeunhomme ◽  
W A Buurman
Keyword(s):  
Mhc Class Ii ◽  
Antigen Expression ◽  
Class Ii ◽  
Ifn Gamma ◽  
Human Leukocytes ◽  
Hla Dr ◽  
Hla Dq ◽  
Mhc Class Ii Antigens ◽  
Class Ii Antigen ◽  
Mhc Class Ii Antigen

In this report, data are presented on the regulation of MHC class II antigen expression by a mediator present in supernatants of human mixed leukocyte cultures (MLC-SN), and which is different from IFN-gamma. The capacity of supernatants to induce antigen expression did not correspond to titers of IFN-gamma. Removal of IFN-gamma using either dialysis against pH 2 or neutralizing mAb against human IFN-gamma did not abrogate the MHC class II antigen expression-inducing capacity of MLC-SN when tested on adenocarcinoma cell lines, kidney epithelial cells, and fibroblasts in vitro in an indirect immunofluorescence assay. Therefore, supernatants of human leukocytes contain a mediator, different from IFN-gamma, which induces expression of MHC class II antigens. Dose-response studies revealed that the mediator is produced after allogeneic and lectin stimulation of human leukocytes, and by unstimulated leukocytes. Activation of leukocytes resulted in increased titers of the mediator. The mediator markedly enhances expression of both HLA-DR and HLA-DQ antigens, whereas IFN-gamma had a similar effect on HLA-DR antigens, and only a minor effect on HLA-DQ antigens. Interaction of the mediator and IFN-gamma resulted in a potentiating effect of these two factors on MHC class II antigen expression. Biochemical analysis revealed a mediator, distinguishable by FPLC from IL-1, IL-2, and human IFN-gamma, and which has a molecular mass of 32 kD.

IN VITRO INDUCTION OF ENDOTHELIAL HLA CLASS II ANTIGEN EXPRESSION BY CYTOMEGALOVIRUS-ACTIVATED CD4+ T CELLS1

1993 ◽  
Vol 56 (6) ◽  
pp. 1504-1512 ◽  
Author(s):  
W. James Waldman ◽  
DEBORAH A. KNIGHT ◽  
PATRICK W. ADAMS ◽  
CHARLES G. OROSZ ◽  
DANIEL D. SEDMAK
Keyword(s):  
Antigen Expression ◽  
Class Ii ◽  
Hla Class Ii ◽  
Class Ii Antigen ◽  
In Vitro Induction

Effect of ecogramostim on HLA class II antigen expression on thyrocytes

The Lancet ◽  
1991 ◽  
Vol 338 (8778) ◽  
pp. 1344 ◽  
Author(s):  
Csaba Balázs ◽  
Agota Bokk ◽  
Edith Bodolay ◽  
NadirR. Farid
Keyword(s):  
Antigen Expression ◽  
Class Ii ◽  
Hla Class Ii ◽  
Class Ii Antigen

Cordyceps sinensisIncreases the Expression of Major Histocompatibility Complex Class II Antigens on Human Hepatoma Cell Line HA22T/VGH Cells

1998 ◽  
Vol 26 (02) ◽  
pp. 159-170 ◽  
Author(s):  
Jen-Hwey Chiu ◽  
Chien-Hue Ju ◽  
Li-Hwa Wu ◽  
Wing-Yiu Lui ◽  
Chew-Wun Wu ◽  
...  
Keyword(s):  
Cell Line ◽  
Mhc Class Ii ◽  
Fluorescence Intensity ◽  
Hepatoma Cell ◽  
Antigen Expression ◽  
Class Ii ◽  
Cordyceps Sinensis ◽  
Hepatoma Cell Line ◽  
Class Ii Antigen ◽  
Mhc Class Ii Antigen

Previous studies suggest that down-regulation of the major histocompatibility complex (MHC) antigens on the cell surface of certain tumors results in an escape of immune surveillance. Cordyceps sinensis is well known for its modulatory effect on host immune system. To investigate the modulatory effect of Cordyceps sinensis on MHC class II antigen expression on hepatoma cells, immunostaining with monoclonal antibody (MAb) L243, against the HLA DR region of MHC class II antigens on human hepatoma cell line HA22T/VGH was analyzed by using flow cytofluorimetry. The degree of fluorescence intensity on L243 (+) cells was expressed as relative mean fluorescence intensity (RMFI). The extract of Cordyceps sinensis (VGH-CS-ME-82, 40 μg/ml) was found to increase the MHC class II antigen expression on HA22T/VGH cells with the percentage of L243 (+) cells 40.2 ± 2.5 and RMFI 6.6 ± 0.4; whereas cells without treatment disclosed the percentage of L243 (+) cells 17.2 ± 1.4 and RMFI 5.4 ± 0.3, respectively (p < 0.05). There was a dose-related increase in the degree of fluorescence intensity in terms of RMFI on VGH-CS-ME-82 induced cells. The RMFI in cells treated with IFN-γ0, 0.2 and 5 ng/ml were 5.4 ± 03, 8.2 ± 0.4, and 24.9 ± 1.5, respectively; whereas the RMFI in cells co-incubated with VGH-CS-ME-82 (40 μg/ml) and IFN-γ0, 0.2 ng/:ml and 5 ng/ml were 6.7 ± 0.2 (p <0.05), 9.2 ± 0.9 (p < 0.1) and 29.5 ± 1.2 (p < 0.005), respectively. We conclude that VGH-CS-ME-82, either alone or with IFN-γ induction, increases the MHC class II antigen expression on hepatoma cell line HA22T/VGH, which will shed light into the present immunotherapy, and make the host immune surveillance more effective against tumor cells with down-regulated MHC class II antigen expression.

The Influence of Triiodothyronine, Thyroxine, Thyrotropin, and Methimazole on Thyroid Cell MHC Class II Antigen Expression

1995 ◽  
Vol 76 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Mohamed A. Atwa ◽  
Yvonne D. Lukes ◽  
Kalman Salata ◽  
Ekbal M. Abo-Hashem ◽  
Mohamed H. El-Kannishy ◽  
...  
Keyword(s):  
Mhc Class Ii ◽  
Antigen Expression ◽  
Class Ii ◽  
Thyroid Cell ◽  
Class Ii Antigen ◽  
Mhc Class Ii Antigen
Sign in / Sign up

Export Citation Format

Share Document