scholarly journals LIPOIDS AS THE GROWTH-INHIBITING FACTOR IN SERUM

1925 ◽  
Vol 42 (2) ◽  
pp. 143-154 ◽  
Author(s):  
Lillian E. Baker ◽  
Alexis Carrel
Keyword(s):  
Chicken Liver ◽  
Embryonic Tissue ◽  
Tyrode Solution ◽  
Chicken Brain ◽  
Inhibiting Action ◽  
Inhibiting Factor ◽  
Growth Inhibiting

The growth-inhibiting action of serum has been shown to be due largely to the lipoids. Serum from which the lipoids have been removed is much less inhibiting to the growth of fibroblasts in vitro than is the original serum, and only slightly more inhibiting than Tyrode solution. The lipoids extracted from the serum are toxic and more inhibiting to the growth of fibroblasts than the original serum. Lipoids extracted from chicken brain, chicken liver, egg, and embryonic tissue have likewise an inhibiting action.

scholarly journals ACTION OF SERUM ON FIBROBLASTS IN VITRO

1923 ◽  
Vol 37 (6) ◽  
pp. 759-765 ◽  
Author(s):  
Alexis Carrel ◽  
Albert H. Ebeling
Keyword(s):  
Connective Tissue ◽  
Serum Proteins ◽  
Embryonic Tissue ◽  
Tyrode Solution ◽  
Food Material ◽  
Nitrogenous Compounds ◽  
Rate Of Growth

It may be concluded that, under the conditions of the experiments: 1. The duration of life of fibroblasts is not altered by the presence of 7 per cent serum in a medium composed of fibrin and Tyrode solution, but is slightly decreased when the concentration of the serum reaches 25 per cent. 2. Fibroblasts cultivated in serum or in Tyrode solution are only in a condition of survival; they do not build up new protoplasm from the serum proteins and their mass does not increase. 3. When embryonic tissue juice is added to the medium, the tissues increase in mass. But the rate of growth is the same in media containing 0 per cent and 10 per cent serum. In 25 per cent serum, however, the rate of growth slightly decreases. Even in the presence of embryonic tissue juice, serum does not increase the rate of growth of connective tissue. 4. The nitrogenous compounds contained in serum are not used as food material by fibroblasts growing in vitro.

scholarly journals HEAT AND GROWTH-INHIBITING ACTION OF SERUM

1922 ◽  
Vol 35 (5) ◽  
pp. 647-656 ◽  
Author(s):  
Alexis Carrel ◽  
Albert H. Ebeling
Keyword(s):  
Inhibiting Action ◽  
Homologous Serum ◽  
Growth Inhibiting

The inhibiting action of homologous serum on the proliferation of fibroblasts in vitro was increased after the serum had been heated at 56° and 70°C. This action decreased after the serum had been heated at 100°C.

scholarly journals THE CULTIVATION OF TISSUES FOR PROLONGED PERIODS IN SINGLE FLASKS

1936 ◽  
Vol 64 (1) ◽  
pp. 121-130 ◽  
Author(s):  
Raymond C. Parker
Keyword(s):  
Cell Death ◽  
Chick Embryo ◽  
Connective Tissue ◽  
Breast Muscle ◽  
Cell Multiplication ◽  
Tyrode Solution ◽  
Low Level ◽  
Further Development

1. Fragments of breast muscle from a 12 day old chick embryo have been kept alive in single flasks for an entire year without being transferred. The nutrient materials were supplied by frequent applications of adult fowl serum diluted with Tyrode solution. 2. When fragments of fixed tissues are cultivated in serum, cell multiplication and cell death are both reduced to an extremely low level. 3. The presence of a plasma coagulum is not essential to the continued survival and further development of tissues cultivated inserum. 4. The fibrinogen, prothrombin, and fibrin of coagulated plasma are not essential to the development of connective tissue fibers in vitro.

Radioreceptor and biological characterization of cholecystokinin and gastrin in the chicken

1984 ◽  
Vol 246 (3) ◽  
pp. G296-G304
Author(s):  
S. R. Vigna
Keyword(s):  
Acid Secretion ◽  
Gastric Acid Secretion ◽  
Gastric Acid ◽  
Amylase Secretion ◽  
Physiological Regulation ◽  
Chicken Brain ◽  
Specific Radioimmunoassay ◽  
Cck Receptor Antagonist

Radioimmunoassay, radioreceptor assays, and bioassays were used to demonstrate that chicken brain and antrum extracts contain cholecystokinin (CCK)-like and gastrinlike peptides, respectively. C-terminal-specific radioimmunoassay of partially purified chicken CCK and gastrin gave dilution curves parallel to those of the mammalian peptides. Mouse cerebral cortical and rat pancreatic membrane radioreceptor assays were used to differentiate CCK- from gastrinlike peptides on the basis of the different CCK versus gastrin specificities of the two receptors. Confirmation of the biological activity of chicken brain CCK was obtained by stimulation of amylase secretion from rat pancreatic lobules in vitro. The specificity of this response was demonstrated by the inhibition of chicken CCK-stimulated amylase secretion by the specific CCK receptor antagonist dibutyryl cGMP. Chicken antral gastrin stimulated gastric acid secretion from the rat stomach in vivo. In contrast to previous hypotheses, it is proposed that chickens have significant amounts of an antral gastrinlike peptide and that therefore it is possible that gastrin is involved in the physiological regulation of gastric acid secretion in chickens.

scholarly journals In vitro RNA synthesis and expression of vitellogenin gene in isolated chicken liver nuclei

1978 ◽  
Vol 5 (4) ◽  
pp. 1353-1370 ◽  
Author(s):  
Sakoi Panyim ◽  
Takeshi Ohno ◽  
Jean-Pierre Jost
Keyword(s):  
Rna Synthesis ◽  
Chicken Liver ◽  
Liver Nuclei ◽  
Vitellogenin Gene
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