scholarly journals SPECIFIC PRECIPITATION AND MOUSE PROTECTION IN TYPE I ANTIPNEUMOCOCCUS SERA

1930 ◽  
Vol 52 (4) ◽  
pp. 477-483 ◽  
Author(s):  
Michael Heidelberger ◽  
Richard H. P. Sia ◽  
Forrest E. Kendall

1. A rapid and simple method is given for the approximate determination of the specifically precipitable protein in Type I antipneumococcus sera. 2. It is shown that a close parallel exists between the specifically precipitable protein and the number of mouse protection units in a wide variety of Type I antipneumococcus sera. 3. Owing to the consistent results obtained and the rapidity, simplicity, and economy of the method, its use is proposed instead of the mouse protection test as a basis for the titration of standard sera and the comparison of others with a standard. 4. A method is given for conveniently preparing highly purified specific polysaccharide of Type I pneumococcus.

The Analyst ◽  
1906 ◽  
Vol 31 (362) ◽  
pp. 147 ◽  
Author(s):  
Cecil H. Cribb ◽  
F. W. F. Arnaud

2010 ◽  
Vol 43 (6) ◽  
pp. 1426-1430 ◽  
Author(s):  
Yudong Zhang ◽  
Zongbin Li ◽  
Claude Esling ◽  
Jacques Muller ◽  
Xiang Zhao ◽  
...  

The fundamental theory of crystal twinning has been long established, leading to a significant advance in understanding the nature of this physical phenomenon. However, there remains a substantial gap between the elaborate theory and the practical determination of twinning elements. This paper proposes a direct and simple method – valid for any crystal structure and based on the minimum shear criterion – to calculate various twinning elements from the experimentally determined twinning plane for Type I twins or the twinning direction for Type II twins. Without additional efforts, it is generally applicable to identify and predict possible twinning modes occurring in a variety of crystalline solids. Therefore, the present method is a promising tool to characterize twinning elements, especially for those materials with complex crystal structure.


1937 ◽  
Vol 66 (4) ◽  
pp. 413-424 ◽  
Author(s):  
Kenneth Goodner ◽  
Frank L. Horsfall

The ability to carry out with some measure of precision mouse protection tests for the estimation of potency of antipneumococcus sera has made possible the correlation of the protective potency with the amount of specifically precipitable protein. With antipneumococcus rabbit sera these protective ratios are relatively constant and higher than those with immune horse serum. Type I antipneumococcus horse sera, on the other hand, show no such constancy but fall into two groups; and there is as yet no simple method for determining to which group a serum belongs.


1936 ◽  
Vol 63 (5) ◽  
pp. 737-744 ◽  
Author(s):  
Michael Heidelberger ◽  
Elvin A. Kabat

1. The absolute, quantitative agglutinin method has been used for the determination of the presence or absence of small amounts of specific polysaccharide in pneumococcus variants. 2. A technique is described for the removal of group specific antibody from antipneumococcus horse serum. 3. The type specific anticarbohydrate agglutinin and precipitin are not only present in identical amounts in Type I antipneumococcus horse serum, but a reduction in one is also accompanied by a quantitatively identical reduction in the other, providing evidence for their actual identity. In purified antibody solutions somewhat more agglutinin than precipitin is found, possibly owing to alteration of a portion of the antibody in the process of purification.


2010 ◽  
Vol 34 (8) ◽  
pp. S75-S75
Author(s):  
Weifeng Zhu ◽  
Zhuoqi Liu ◽  
Daya Luo ◽  
Xinyao Wu ◽  
Fusheng Wan

1977 ◽  
Vol 12 (1) ◽  
pp. 27-50 ◽  
Author(s):  
L.A. Behie ◽  
J.E. Zajic ◽  
D. Berk ◽  
R.J.P. Brouzes ◽  
V.A. Naish

Abstract Although Daphnia magna have been widely used in the determination of the toxicity of various substances, there are no reports in the literature that describe a rigorous bioassay method using this organism as a test species. The test described herein involves the standariza-tion of various important aspects of the method such as the age of the test organisms, and the dilution water used for the preparation of the various toxicant concentrations. Also described is a simple method for the statistical analysis of the results. The sensitivity of the proposed bioassay is demonstrated by determining the toxicity of various pulp and paper effluents. Finally, extensive bioassays were carried out simultaneously with rainbow trout and Daphnia magna indicating that Daphnia are as good a biological indicator of acute toxicity as fish.


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