High-throughput X-ray fluorescence imaging using a massively parallel detector array, integrated scanning and real-time spectral deconvolution

Telomere length is a promising biomarker for age-associated diseases and cancer, but there are still substantial challenges to routine telomere analysis in clinics because of the lack of a simple and rapid yet scalable method for measurement. We developed the single telomere absolute-length rapid (STAR) assay, a novel high-throughput digital real-time PCR approach for rapidly measuring the absolute lengths and quantities of individual telomere molecules. We show that this technique provides the accuracy and sensitivity to uncover associations between telomere length distribution and telomere maintenance mechanisms in cancer cell lines and primary tumors. The results indicate that the STAR assay is a powerful tool to enable the use of telomere length distribution as a biomarker in disease and population-wide studies.

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The Maia detector array and x-ray fluorescence imaging system: locating rare precious metal phases in complex samples

10.1117/12.2027195 ◽

2013 ◽

Cited By ~ 12

Author(s):

C. G. Ryan ◽

D. P. Siddons ◽

R. Kirkham ◽

Z. Y. Li ◽

M. D. de Jonge ◽

...

Keyword(s):

Fluorescence Imaging ◽

Precious Metal ◽

Imaging System ◽

Detector Array ◽

Complex Samples ◽

X Ray ◽

Fluorescence Imaging System ◽

Metal Phases

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A unified approach for high throughput analysis of real-time biomolecular interactions in surface plasmon resonance and fluorescence imaging

10.1117/12.877597 ◽

2011 ◽

Author(s):

Catalin Fetita ◽

Nicolas François ◽

Françoise Prêteux ◽

Hervé Delacroix

Keyword(s):

Surface Plasmon Resonance ◽

Real Time ◽

Fluorescence Imaging ◽

Surface Plasmon ◽

High Throughput ◽

Plasmon Resonance ◽

Biomolecular Interactions ◽

Unified Approach ◽

High Throughput Analysis ◽

Throughput Analysis

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Nanoscale “Curtain Rods”: High-Throughput Tools for Studying DNA-Protein Interactions

MRS Proceedings ◽

10.1557/proc-1138-ff08-03 ◽

2008 ◽

Vol 1138 ◽

Author(s):

Teresa Fazio ◽

Mari-Liis Visnapuu ◽

Shalom J. Wind ◽

Eric Greene

Keyword(s):

Data Acquisition ◽

Real Time ◽

Lipid Bilayer ◽

High Throughput ◽

Single Molecule ◽

Protein Interactions ◽

Massively Parallel ◽

Dna Interactions ◽

Parallel Data ◽

Protein Dna Interactions

AbstractIn this work, we combine nanoscale engineering with single-molecule biology to probe the biochemical interactions between individual proteins and DNA. This approach, a vast improvement over previous methods, constructs a platform to observe thousands of protein-DNA interactions in real time with unprecedented detail. A key challenge in these experiments involves collecting enough statistically relevant data in order to analyze reactions which are designed to be probed individually. “DNA curtains” are formed by flowing the DNA tethered to a lipid bilayer across nanopatterned barriers, facilitating massively parallel data acquisition.

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A Large Silicon Detector Array and Real-Time Elemental Image Projection of X-ray Microprobe Fluorescence Data Using Pipelined, Parallel Processing

Microscopy and Microanalysis ◽

10.1017/s1431927605510298 ◽

2005 ◽

Vol 11 (S02) ◽

Author(s):

D P Siddons ◽

C G Ryan ◽

P A Dunn ◽

A Broadbent

Keyword(s):

Parallel Processing ◽

Real Time ◽

Silicon Detector ◽

Detector Array ◽

X Ray ◽

Image Projection ◽

Elemental Image

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High-throughput data acquisition and processing for real-time x-ray imaging

10.1117/12.2237611 ◽

2016 ◽

Cited By ~ 1

Author(s):

Matthias Vogelgesang ◽

Lorenzo Rota ◽

Luis E. Ardila Perez ◽

Michele Caselle ◽

Suren Chilingaryan ◽

...

Keyword(s):

Data Acquisition ◽

Real Time ◽

High Throughput ◽

X Ray ◽

High Throughput Data ◽

X Ray Imaging ◽

Data Acquisition And Processing

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Large detector array and real-time processing and elemental image projection of X-ray and proton microprobe fluorescence data

Nuclear Instruments and Methods in Physics Research Section B Beam Interactions with Materials and Atoms ◽

10.1016/j.nimb.2007.01.271 ◽

2007 ◽

Vol 260 (1) ◽

pp. 1-7 ◽

Cited By ~ 29

Author(s):

C.G. Ryan ◽

D.P. Siddons ◽

G. Moorhead ◽

R. Kirkham ◽

P.A. Dunn ◽

...

Keyword(s):

Real Time ◽

Detector Array ◽

Real Time Processing ◽

Time Processing ◽

X Ray ◽

Proton Microprobe ◽

Image Projection ◽

Elemental Image

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A polynuclear Cu(ii) complex for real time monitoring of mitochondrial cytochrome C release during cellular apoptosis

Chemical Communications ◽

10.1039/d0cc01606c ◽

2020 ◽

Vol 56 (48) ◽

pp. 6563-6566

Author(s):

Somnath Khanra ◽

Sabyasachi Ta ◽

Ankush Paladhi ◽

Milan Ghosh ◽

Subhasis Ghosh ◽

...

Keyword(s):

Cytochrome C ◽

Single Crystal ◽

Real Time ◽

Fluorescence Imaging ◽

Live Cells ◽

Mitochondrial Cytochrome ◽

Cytochrome C Release ◽

X Ray ◽

Cellular Apoptosis ◽

Intracellular Translocation

Single crystal X-ray structurally characterized poly-nuclear Cu(ii) complex (M1) selectively and spatially interacts with Cytochrome C (Cyt C) to allow fluorescence imaging of intracellular translocation events in live cells during apoptotic process.

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