scholarly journals Development of electronic modules for their integration with impedimetric transducers applied to sarcosine detection

2021 ◽  
Vol 2008 (1) ◽  
pp. 012006
Author(s):  
A Perez-Nava ◽  
V Vallejo-Becerra ◽  
S Fernández-Puig ◽  
G Oza ◽  
J Herrera-Celis

Abstract The development of fast, simple, sensitive, and minimally invasive biosensors for detecting diseases, conventionally need specialized, expensive, and highly invasive instrumentation. Furthermore, such biosensors pertinently also, need the development of optoelectronic modules that are capable of implementing specific detection techniques while interacting with the user through a friendly interface. This work highlights the development of a system whose hardware and software contributes to the detection of analytes by impedimetric sensors, especially emphasizing on the detection of sarcosine, a natural amino acid associated with prostate cancer (PCa). Dummy circuits coupled with impedimetric transducers were used to perform precise measurements using a sinusoidal signal of 20 mV in the range from 0.1 Hz to 1 MHz.

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 421
Author(s):  
Areetha R. D’Souza ◽  
Matthew R. Necelis ◽  
Alona Kulesha ◽  
Gregory A. Caputo ◽  
Olga V. Makhlynets

Antimicrobial peptides (AMPs) present a promising scaffold for the development of potent antimicrobial agents. Substitution of tryptophan by non-natural amino acid Azulenyl-Alanine (AzAla) would allow studying the mechanism of action of AMPs by using unique properties of this amino acid, such as ability to be excited separately from tryptophan in a multi-Trp AMPs and environmental insensitivity. In this work, we investigate the effect of Trp→AzAla substitution in antimicrobial peptide buCATHL4B (contains three Trp side chains). We found that antimicrobial and bactericidal activity of the original peptide was preserved, while cytocompatibility with human cells and proteolytic stability was improved. We envision that AzAla will find applications as a tool for studies of the mechanism of action of AMPs. In addition, incorporation of this non-natural amino acid into AMP sequences could enhance their application properties.


2008 ◽  
Vol 16 (24) ◽  
pp. 10257-10269 ◽  
Author(s):  
Georgia Antonopoulou ◽  
Efrosini Barbayianni ◽  
Victoria Magrioti ◽  
Naomi Cotton ◽  
Daren Stephens ◽  
...  

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