scholarly journals In vitro shoot multiplication of Morus nigra by combinations of plant growth regulators

2021 ◽  
Vol 921 (1) ◽  
pp. 012036
Author(s):  
S H Larekeng ◽  
M A Arsyad ◽  
A M Annisa ◽  
M Restu
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Shoot Multiplication ◽  
Callus Cultures ◽  
Leaf Length ◽  
Plant Parts ◽  
Morus Nigra ◽  
Conventional Technology ◽  
The Right

Abstract Mulberry (Morus nigra L) is a dicotyledonous plant in the family Moraceae which often used for silkworms’ breeding. Mulberry propagation still uses conventional technology, such as cuttings and graftings. The problem of mulberry propagation is the low productivity of mulberry gardens. However, tissue culture is a technique of isolating plant parts in the form of organs, cell tissues, and protoplasms that is effective and efficient in order to get uniform and superior plants in a short amount of time. For mulberry growth, the right combination of the Plant Growth Regulator (PGR) in the form of Kinetin, IAA, IBA is needed. Data were analyzed using R-statistic software. The results showed that M6 media (MS + Kinetin 1.5 + 1 IAA) was the best combination of media for the number of shoots, leaf length, and number of roots, with 80% of live explant percentage. Propagation via tissue culture such as clonal propagation, organogenesis, and somatic embryogenesis in callus cultures followed by generation of shoots/plantlets represents a potential effective propagation method in the future.

scholarly journals Pertumbuhan dan Perkembangan Protocorn Like Bodies (PLB) Anggrek Papua Dendrobium discolor dengan Perlakuan Auksin dan Sitokinin

2021 ◽  
Vol 13 (1) ◽  
pp. 28-35
Author(s):  
Agustriani Warpur ◽  
Linus Y. Chrystomo ◽  
Suharno Suharno
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Combination Treatment ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Leaf Length ◽  
Significant Treatment ◽  
Culture Techniques ◽  
Growth Regulator Treatment

Dendrobium could meet the demands of domestic and foreign consumers from time to time because of their very charming flower blossoms. Orchid flowers are attractive because of the variety their color, size, shape, composition, number of buds the blossom of flowers, especially for the color it is vary greatly. One of the potential Dendrobium Papua orchids is Dendrobium discolor. To fulfill the needs of Dendrobium orchids in pots or in peaces it is depend on the success of Protocorn Like Bodies (PLB) cultivation. The purpose of this study was to determine the growth and development of PLB cultivated in vitro on Vacint & Went (V&W) base media with a combination of different plant growth regulators. The method used wasPLB cultivation with tissue culture techniques in vitro on V&W media with a factorial completely randomized design (CRD). The first factor was auxin treatment (2,4D) with 5 concentrations 1, 2, 3, and 4 ppm plus control (without plant growth regulator treatment), while the second factor was cytokinin treatment with 5 concentrations 1, 2, 3, and 4 ppm plus 1 control (without plant growth regulator treatment). Each treatment repeated 5 times, threfore, there are 125 treatment units in total 125 treatment units. The media used were Vacin and Went (VW). The variables measured were leaf length and the number of leaves per plantlet in each combination treatment of plant growth regulator (PGR) after one month of cultivation. Data were analyzed by using variance (ANOVA) to determine the significance. The results of this study indicated that the combination treatment of auxin and cytokinin PGR in V&W media had a significant effect on the length and number of plantlets leaves and the most significant treatment was the combination of PGR auxin: cytokinin = 0:3 ppm. So it can be concluded that the best medium for PLB culture is a combination of PGR auxin: cytokinin = 0:3 ppm. with an average value of 3.5 cm plantlet leaf length and number of 6 leaves per plantlet. Key words: Protocorn Like Bodies; tissue culture; V&W medium; auxins; cytokinins.

scholarly journals Rapid Multiplication of Rauvolfia serpentina Benth. Ex. Kurz through Tissue Culture

1970 ◽  
Vol 6 (6) ◽  
pp. 58-62 ◽  
Author(s):  
Krishna K Pant ◽  
Sanu D Joshi
Keyword(s):  
Tissue Culture ◽  
Adventitious Shoots ◽  
Shoot Multiplication ◽  
Callus Cultures ◽  
Culture Technique ◽  
Conservation Strategy ◽  
Ex Situ ◽  
Ex Vitro ◽  
Rauvolfia Serpentina

Rauvofia serpentina Benth. ex Kurz (Apocynaceae) is a highly important medicinal plant growing in the terai belts of Nepal. This plant is facing a high threat from various kinds of poachers in the wild due to improper ways of collection as well as almost no conservation strategy. In the present study, we have identified the way for rapid in vitro multiplication of this species using tissue culture technique as an effective tool for ex situ conservation. In this study, MS medium along with BAP and NAA alone or in combinations at different concentrations have given successful results in producing callus, multiple adventitious shoots both from callus and nodes and multiple roots from both callus as well as nodes. The in vitro as well as ex vitro rooting of the micro shoots have been achieved. The rooted seedlings have been successfully acclimatized. The best media for shoot multiplication from the node and callus cultures have been identified as MS + NAA 0.5 + BAP 0.5 ppm and MS + BAP 2.0 ppm, respectively. For rooting in and ex vitro, NAA has been found to be the best among auxins. For callus induction all the auxins specially NAA 1.0 ppm and 2,4-D 2.0 ppm have been found to be the best.  Keywords: Micropropagation; Rauvolfia serpentina; In vitro rooting; Pulse treatment and acclimatization.   DOI: 10.3126/sw.v6i6.2635 Scientific World, Vol. 6, No. 6, July 2008 58-62

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

scholarly journals Effects of Sucrose and Other Additives on In Vitro Growth and Development of Purple Coneflower (Echinacea purpurea L.)

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Dahanayake Nilanthi ◽  
Yue-Sheng Yang
Keyword(s):  
Plant Growth ◽  
Culture Medium ◽  
Respiratory Infections ◽  
Shoot Multiplication ◽  
Echinacea Purpurea ◽  
Naphthaleneacetic Acid ◽  
Acid Plant ◽  
Purple Coneflower ◽  
Upper Respiratory

Echinacea purpurea (purple coneflower) is being used for the preparation of more than 240 extracts, salves, and tinctures to help cure diseases like rabies, cold, and upper respiratory infections. Hence, efforts were made to develop a culture medium for successful in vitro culturing of cornflower and to regenerate buds and induce roots to enable mass propagation of selected clones. Of the three levels of sucrose tested as a supplement to MS media (Murashige and Skoog’s medium, 1962) 3% showed better rooting of buds and appeared morphologically normal and identical as compared to those grown at higher and lower concentrations (2 and 4%). The additives hydrolyzed lactabumin (0.0, 100, 300, and 900 mgL−1), peptone (0.0, 100, 300, and 900 mgL−1), and yeast (0.0, 100, 300, and 900 mgL−1) to media containing 0.3 mgL−1 BA (6-benzyladenine) and 0.01 mgL−1 NAA (naphthaleneacetic acid-plant growth regulators) has negatively influenced proliferation of shoots. The higher concentrations of the above have delayed the development of plantlets. Shoot multiplication was enhanced by coconut water with 2% being the best among 4 and 8% tested. Shoot organogenesis was not influenced by copper sulphate (0, 1.5, 3, 6, and 12 mgL−1) and silver nitrate (0.0, 0.5, 2.5, and 12.5 mgL−1) supplements and at higher concentrations of the above inhibited plant growth.

Effects of Different Plant Growth Regulator on in vitro Shoot Multiplication of Chrysanthemum morifolium

2003 ◽  
Vol 3 (6) ◽  
pp. 553-560 ◽  
Author(s):  
M.Z. Karim . ◽  
M.N. Amin . ◽  
M.A.K. Azad . ◽  
F. Begum . ◽  
M.M. Rahman . ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Shoot Multiplication ◽  
Chrysanthemum Morifolium

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

scholarly journals In vitro Multiplication of the Pitcher Plant Sarracenia Purpurea

2018 ◽  
Vol 75 (2) ◽  
pp. 134
Author(s):  
Ileana MICLEA ◽  
Rita BERNAT
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Multiplication ◽  
Time Frame ◽  
Naphthaleneacetic Acid ◽  
Sarracenia Purpurea ◽  
Pitcher Plant ◽  
Frame Number

The aim of the current research was to find the best plant growth regulators for the multiplication of Sarracenia purpurea. Murashige and Skoog medium (MS) was prepared with macronutrients and micronutrients at 1/3 strength, full strength vitamins, supplemented with 30 g/l sucrose and 5 g/l phytagel and autoclaved. After cooling 0.5 mg\l α-naphthaleneacetic acid (NAA), 5 mg\l 6-benzyladenine (BA) or 0.5 mg\l NAA + 3 mg\l BA were added. Young S. purpurea plants were selected and transferred to media with or without plant growth regulators and cultured for 12 weeks. At the end of this time frame number of roots, root length (cm) and number of shoots were evaluated and differences were analysed by the analysis of variance and interpreted using the Tuckey test. The largest number of roots grew in medium supplemented with 0.5 mg\l NAA but the the absence of plant growth regulators increased their length. The best conditions for shoot multiplication were provided by supplementing 1/3MS with 5 mg\l BA.

scholarly journals An efficient in vitro propagation protocol for snowdrop anemone (Anemone sylvestris L.) 

2017 ◽  
Vol 44 (No. 4) ◽  
pp. 186-194 ◽  
Author(s):  
Jana Šedivá ◽  
Pavla Zahumenická ◽  
Eloy Fernández Cusimamani
Keyword(s):  
Plant Growth ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Growth Characteristics ◽  
Root Induction ◽  
Shoot Induction ◽  
Free Medium ◽  
In Vitro Production ◽  
Vitro Production

This study investigated in vitro production of diploid (AS2) and tetraploid (AS4) cytotypes of snowdrop anemone. The effect of plant growth regulators (PGRs) on in vitro shoot multiplication and rooting was investigated. The effect of activated charcoal (AC) on root induction was also studied. Ploidy level affected growth characteristics during multiplication and rooting. Shoot induction in AS4 was higher on medium supplemented with cytokinin (3.2–3.6), while the AS2 clone formed the most shoots on PGR-free medium (3.6). The highest rooting percentage was achieved on PGR-free medium in both genotypes (AS2 clone, 100% and AS4 clone, 93.3%). The addition of AC to the PGR media largely increased root induction and root length. Rooted plantlets were successfully acclimatised in the greenhouse with 100% survival. Thus, the described micropropagation protocol represents a rapid and effective in vitro propagation method for utilisation in horticulture and conservation programmes of snowdrop anemone.

scholarly journals Organogenesis in okra (Abelmoschus esculentus L. Moench.): A plant recalcitrant to tissue culture

2016 ◽  
Vol 41 (3) ◽  
pp. 521-528
Author(s):  
MR Kabir ◽  
S Ahmed ◽  
MAY Akhond
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Growth ◽  
Root Induction ◽  
Ms Medium ◽  
Natural Conditions ◽  
Hypocotyl Explants ◽  
Cotyledonary Nodes ◽  
Dichlorophenoxy Acetic Acid

Seedling-derived cotyledonary nodes and hypocotyl explants of BARI Dherosh- 1 were cultured in vitro on MS medium supplemented with varying concentrations of 2, 4-Dichlorophenoxy acetic acid (2, 4-D), 6- Benzylaminopurine (BAP), Thidiazuron (TDZ), BAP with 1-Nepthaleneacetic acid (NAA), BAP with Indole 3-butyric acid (IAA) and Zeatin with IAA along with a control. Shooting response (100%) with callus was only observed from cotyledonary nodes on thidiazuron (TDZ) where hypocotyls produced only callus or callus with roots on different concentrations of plant growth regulators. Considering the shooting response, the cotyledonary nodes of BARI Dherosh-1 were cultured on various concentrations of TDZ for regeneration. The highest percentage (64.0) with maximum number (6.8) of shoots per explant were observed in 0.044 ?M TDZ in 8.4 days. The regenerated shoots were rooted on ½ strength MS, MS supplemented with 2.46 ?M IBA and 0.53 ?M NAA. The highest percentage (83.3) and minimum days (9.7) required for root induction were recorded in 2.46 ?M IBA. The rooted plantlets were transferred to soil and hardened in the plastic pots under green house conditions. The rooted shoots grew normally under natural conditions following acclimatization.Bangladesh J. Agril. Res. 41(3): 521-528, September 2016

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