Biomonitoring of air quality in the Bodoquena microregion, Mato Grosso Do Sul: mutagenic and morphoanatomical alterations in Tradescantia pallida (rose) D.R. Hunt var. purpurea

The objective of this study was to assess air quality in relation to vehicular traffic flow in cities located at different elevations in the Bodoquena microregion, state of Mato Grosso do Sul, Brazil. To do so, a micronucleus test was carried out using the TRAD-MCN bioassay on young Tradescantia buds collected from February to November 2018 in seven cities of the microregion with different traffic flow intensities. Meteorological parameters were evaluated, and vehicular traffic was counted to determine traffic flow in each city. With data from the Shuttle Radar Topography Mission (SRTM) and processing in Esri ArcGIS® software version 10.5.1, the regions was mapped based on an Elevation Model. Morphoanatomical analyses were performed according to standard methodology. Measurements were taken of thickness, length and width of tissues and structures, including the upper and lower cuticle, upper and lower epidermis, hypodermis and mesophyll. The greatest traffic flow was found in the cities of Bodoquena, Guia Lopes da Laguna, Jardim, and Porto Murtinho, with the period from 5:00 to 6:00 p.m. showing the highest traffic flow. The greatest frequency of mutagenic alterations was found in the city of Guia Lopes da Laguna, although the results did not differ significantly from Bonito, Caracol, and Jardim. Throughout the biomonitoring, the summer and autumn seasons showed the greatest micronuclei frequencies in all evaluated cities. Variations in the tissue/structure thickness was observed across cities and seasons, but with a decrease in thickness during autumn. In general, the tissues/structures were smaller for the cities of Nioaque and Porto Murtinho, while the anatomical and morphological characteristics of leaf length and thickness showed no differences among cities. We found limited correlation between micronuclei frequency and traffic flow, supporting the hypothesis that although mutagenic alterations are observed in T. pallida, in this microregion the changes are numerically lower when compared to other regions of the state. In light of the genotoxic and morphoanatomical factors assessed herein, the Bodoquena microregion appears to be well preserved in terms of air quality, presenting low micronuclei frequency and a limited reduction in tissues and leaf structures, regardless of the season.

Correlation between Genetic Characteristics, Cell Structure and Material Properties of Moso Bamboo (Phyllostachys edulis (Carriere) J. Houzeau) in Different Areas of China

Moso bamboo (Phyllostachys edulis (Carriere) J. Houzeau), native to China, is one of the most economically and ecologically important bamboo species. Since the economic interests and the strong clonality, it has been widely cultivated in southern China, which inevitably reduces the natural stands and leads to gene loss in this species. In this study, three natural populations of Moso bamboo distributed in Anhui, Guangxi, and Zhejiang province, were used to analyze the correlation between phenotypic traits, cell structure, and material properties from the perspective of phenotypic, genetic, and environmental. Among those traits and properties, fiber width was correlated with wall thickness at breast height and average nodes length under branch positively. Leaf length was correlated positively with fiber lumen diameter and parenchyma lumen diameter. Furthermore, it showed a very close correlation between moisture content, bending strength, modulus of elasticity, and diameter at breast height, clear height, and leaf length. The lumen diameter of fiber cell wall thickness is positively correlated with bending strength and modulus of elasticity. Density is positively correlated with parenchyma cell wall thickness. The experimental design is relatively detailed and representative, and the workload is huge. This study reflects the research objectives with scientific and rational experiments and data. This study will analyze the differences of various indicators from the perspective of genetic to build a bridge between micro-structure and macro-structure for rational utilization of the whole area of Moso bamboo resources in China.

Pengaruh Pupuk Sampah Kota dan dan Pupuk Kandang Sapi terhadapTinggi Tanaman, Panjang Daun, dan Berat Panen Tanaman Pakcoy (Brasica rapa ) pada Andisol di Kabupaten Indramayu

The objective of this research was to find out the effect of municipal solid waste compost and cow manure on plant height, leaf length, and harvest weight of pakcoy (Brasica rapa) in Andisols in Indramayu Regency. This study used a randomized block design factorial with two factors. The first factor is municipal waste compost and the second factor is cow manure. Each of them consisted of 4 levels : 0%, 3.0%, 6.0%, and 9.0% with two replication. The results showed that there was an interaction between municipal solid waste compost and cow manure on the plant heights of pakcoy 14, 21, and 28 HST, but there was no interaction at 7 HST. The statistical results show that the combination of municipal solid waste compost with cow manure has an effect on the height of pakcoy 14, 21, and 28 HST, but the height of pakcoy 7 HST is influenced by the independent influence of cow manure. There was an interaction between municipal solid waste compost and cow manure on the leaf length of pakcoy 7, 14, 21, and 28 HST, and there was an interaction between municipal waste compost and cow manure on the harvest weight of pakcoy. The application of organic matter, both municipal solid waste compost and cow manure to the soil can increase plant height, leaf length, and harvest weight of pakcoy.

Evaluation of radish genotypes with special study on sowing geometry at agricultural research institute, Tarnab, Peshawar

The research was carried out on the “Evaluation of Radish genotypes with special study on sowing geometry at Agricultural Research Institute, Tarnab Peshawar”. The experiment was laid out in randomized complete block design having three replications. The experiment comprised with two factors, one was sowing dates(19th Oct., 4th., Nov., and 19th Nov) and the other was different genotypes (Mino, Local Red and Local White). The data were recorded on Days to germination, Germination (%), Days to edible maturity, Number of leaves plant-1, Leaf length plant-1 (cm), Root length plant-1 (cm), Root diameter plant-1 (cm), root fresh weight (g), Plot weight (kg) and yield (t ha-1). Sowing dates significantly affected all yield parameters. Sowing of radish on 19th October has more number of Leaves (17.44 leaves) plant-1, Leaf length plant(22.611 cm), root length plant-1(24.058 cm), root diameter plant-1(3.12 cm) fresh root weight (503.95g), the maximum plot weight (50.90 kg plot-1) and higher yield (62.36t ha-1) was observed whereas the crop sown on 19th November showed the minimum days to germination (8.88 days), germination percentage (96.88%) and minimum days to maturity (53 days). Among genotypes Mino take minimum days to germinate(8.22 days), maximum germination percentage (99.22), minimum days to maturity (52.778), maximum number of leaves (19.44 leaves), leaf length (23.422 cm), greater root length (24.588 cm), maximum root diameter (3.53 cm), higher root fresh weight (536.62g), higher weight per plot (52.22 kg) and maximum yield (63.54t ha-1). The results emphasized that the suitable time of sowing for Mino genotype is 19th October for Peshawar.

Variation of phenotypic and physiological traits of Robinia pseudoacacia L. from 20 provenances

To select elite Robinia pseudoacacia L. germplasm resources for production, 13 phenotypes and three physiological indicators of 214 seedlings from 20 provenances were systematically evaluated and analyzed. The leaf phenotypic and physiological coefficients of variation among the genotypes ranged from 3.741% to 19.599% and from 8.260% to 42.363%, respectively. The Kentucky provenance had the largest coefficient of variation (18.541%). The average differentiation coefficients between and within provenances were 34.161% and 38.756%, respectively. These close percentages showed that R. pseudoacacia presented high genetic variation among and within provenances, which can be useful for assisted migration and breeding programs. Furthermore, based on the results of correlations, principal component analysis and cluster analysis, breeding improvements targeting R. pseudoacacia’s ornamental value, food value, and stress resistance of were performed. Forty and 30 excellent individuals, accounting for 18.692% and 14.019%, respectively, of the total resources. They were ultimately screened, after comprehensively taking into considering leaf phenotypic traits including compound leaf length, leaflet number and leaflet area and physiological characteristics including proline and soluble protein contents. These selected individuals could provide a base material for improved variety conservation and selection.

Exploration and morphologic variation of Iris wild species with ornamental potential

Nowadays, the flower industry is growing so fast that its development requires introducing new varieties to the flower market. Iran is the habitat of numerous species of wild plants that are among the valuable genetics in the breeding process. The identification of native Iris species, investigation of morphological diversity, and distribution method have a significant effect on the introduction of this flower as new and superior cultivars cut flower and bedding plant. The documentation of the most important habitats of Iris in Arak province, identification of morphological characteristics and correlation determination of morphological traits are the most important aims of this research. The results showed that there is a correlation between some of the traits. The highest positive correlation was related to the height of flower and length of the leaf, length of the flowering stem and the length of the leaf and the height of the flower and the length of the flowering stem. The ecotypes of Iris sp. were divided into six groups. Based on the results of the cluster diagram, the bulbous species were separated from rhizome species. Generally, due to the color diversity of Iris sp. and unique beauty of the flowers and the resistance of the native species, they can be used as native foundations. Due to the prominent traits such as high durability of Iris meda, odor of I. hymernospatha Subsp. leptoneura and color diversity of I. persica, I. songarica and height of I. spuria, they can be used to introduce new ornamental species. Iris spuria is the most suitable genotype because of the high height, large flower, high durability, and beautiful flower.

Nutritional content, performance and ratooning ability of four sorghum genotypes

Sorghum is a grain plant that is a source of food, biofuel and feed, which can be produced using the techniques of ratoon cultivation. The objective of this study is to assess the nutrition content, the performance of the four sorghum genotypes and the vegetative characteristics of the sorghum ratoon. The research was carried out from December 2020 to July 2021 at the Ciawi Research Garden, the Livestock Research Institute. The study employed four treatments (genotype) and three replications of random block design. The range of protein content, crude fat crude fiber and ash in the four genotypes of sorghum was 7.91-9.30, 1.91-2.69, 33.41-37.57 and 8.25-9.11%, respectively. Genotype significantly affected P<0.05 on plant height, stem height, leaf width, internode length, flag leaf width and fresh weight, but not significantly different P>0.05 on leaf length, stem diameter and flag leaf length on the main plant. Genotype had no significant effect (P>0.05) on plant height, number of tillers, leaf length, leaf width and fresh weight of ratoons. It was concluded that the ability of ratoon in sorghum was not affected by the genotype of sorghum.

Performance of Putative Mutants and Genetic Parameters of Plectranthus amboinicus (L.) through Mutation Induction With Colchicine

Genetic enhancement in vegetatively propagated crops can be done through mutation induction. Colchicine-induced mutation is one of the methods that can be employed to increase plant genetic diversity. This study aimed to determine the effect of colchicine on the performance and genetic parameters of MV3 generation of Plectranthus amboinicus (L.). This study was conducted at the Laboratory of Agricultural Seed Management, Plantation Research and Development Center, from June 2018 to June 2019. Nodes and shoots were used as explants. Mutation induction was performed using colchicine at concentrations of 0%, 0.02%, 0.04%, and 0.06%. Explant regeneration and subculture were done on MS0 medium. The number of plantlets yielded were 59 (0% concentration of colchicine), 60 (0.02%), 81 (0.04%), and 80 plantlets (0.06%), respectively. Results indicated that colchicine-induced mutation in an in vitro culture was able to generate high genetic diversity in both quantitative and qualitative characters of the plantlets. At the concentration of 0.04%, colchicine produced the highest frequency of putative mutants (28.4%). Genetic parameters in MV3 generation of P. amboinicus plantlets showed that five quantitative characters, i.e. plantlet height, number of leaves, number of shoots, leaf length, and number of roots had high heritability values at a concentration around the LC50 value (0.0275%).

Genetic Variability in Growth Characteristics among Different Clones of Eucalyptus tereticornis Sm.

The present investigation was carried out at the College of Forestry, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India during August, 2018 to June, 2019 to study the genetic variability in growth characteristics among different clones of Eucalyptus tereticornis. Different clones of Eucalyptus tereticornis Sm. were planted in RCBD, with 4 replications revealed significant variations among all eight treatments (clones) with respect to 9 different characters. Based on the mean performance, treatment-1 (clone-526) showed maximum value for characters like biomass (1124.17), plant height (247.9 cm), collar diameter (23.25 mm), and a number of leaves plant-1 (463.25 number). Similarly, the maximum value was observed in treatment-8 (clone-136) for traits like leaf area (42.70 cm2), leaf length (15 cm), and leaf width (5.75 cm). The highest leaf length to leaf width ratio (3.57) and lowest number of branches plant-1 (18.75 number) were found in treatment-2 (clone-288). All characters had exhibited higher genotypic variance than an environmental variance. Similarly, the genetic coefficient of variation in the case of all variables was also found greater than an environmental coefficient of variation. Heritability was found maximum in plant height (87.35%) and all other characters also showed high heritability. Genetic advance as % of mean was found maximum in biomass (71.15%). Based on the overall mean performance of growth characters, Treatment-1 (Clone-526) was found as a superior clone with respect to the most important character biomass for the test locality. High GCV, heritability, and GAM value for biomass indicated that the character would respond to selection for the improvement program.

The effect of combination of AB-mix nutrition with liquid organic fertilizer of tofu liquid waste on hydroponical growth and production of lettage (Lactuca sativa L.)

This study aims to determine the effect of the combination of AB mix nutrients with liquid organic fertilizer (LOF) of tofu liquid waste on the growth and yield of lettuce plants and to obtain the best combination for the growth and yield of lettuce hydroponically. This study used a non-factorial Randomized Block Design (RBD), consisting of a comparison of nutritional treatments, namely: N1 = 100% AB mix + 0% LOF of liquid waste tofu, N2= 75% AB mix + 25% LOF of tofu liquid waste, N3= 50% AB mix + 50% LOF of tofu liquid waste, N4= 25% AB mix + 75 % LOF of tofu liquid waste, N5= 100% LOF of waste liquid tofu which consists of 4 replications. Parameters observed were plant height, number of leaves, leaf length, leaf width, root length, fresh weight, root volume and weight fit for consumption. The results showed that the administration of 50% AB mix + 50% LOF of tofu liquid waste and 25% AB mix + 75% LOF of tofu liquid waste showed results that tended to be the same but the results of the combination of 75% AB mix + 25% LOF of tofu liquid waste were higher. On the parameters of the number of leaves is 11.12 strands, fresh weight is 66.40 g and suitable weight for consumption is 58.31 g.