scholarly journals Pertumbuhan dan Perkembangan Protocorn Like Bodies (PLB) Anggrek Papua Dendrobium discolor dengan Perlakuan Auksin dan Sitokinin

2021 ◽  
Vol 13 (1) ◽  
pp. 28-35
Author(s):  
Agustriani Warpur ◽  
Linus Y. Chrystomo ◽  
Suharno Suharno
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Combination Treatment ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Leaf Length ◽  
Significant Treatment ◽  
Culture Techniques ◽  
Growth Regulator Treatment

Dendrobium could meet the demands of domestic and foreign consumers from time to time because of their very charming flower blossoms. Orchid flowers are attractive because of the variety their color, size, shape, composition, number of buds the blossom of flowers, especially for the color it is vary greatly. One of the potential Dendrobium Papua orchids is Dendrobium discolor. To fulfill the needs of Dendrobium orchids in pots or in peaces it is depend on the success of Protocorn Like Bodies (PLB) cultivation. The purpose of this study was to determine the growth and development of PLB cultivated in vitro on Vacint & Went (V&W) base media with a combination of different plant growth regulators. The method used wasPLB cultivation with tissue culture techniques in vitro on V&W media with a factorial completely randomized design (CRD). The first factor was auxin treatment (2,4D) with 5 concentrations 1, 2, 3, and 4 ppm plus control (without plant growth regulator treatment), while the second factor was cytokinin treatment with 5 concentrations 1, 2, 3, and 4 ppm plus 1 control (without plant growth regulator treatment). Each treatment repeated 5 times, threfore, there are 125 treatment units in total 125 treatment units. The media used were Vacin and Went (VW). The variables measured were leaf length and the number of leaves per plantlet in each combination treatment of plant growth regulator (PGR) after one month of cultivation. Data were analyzed by using variance (ANOVA) to determine the significance. The results of this study indicated that the combination treatment of auxin and cytokinin PGR in V&W media had a significant effect on the length and number of plantlets leaves and the most significant treatment was the combination of PGR auxin: cytokinin = 0:3 ppm. So it can be concluded that the best medium for PLB culture is a combination of PGR auxin: cytokinin = 0:3 ppm. with an average value of 3.5 cm plantlet leaf length and number of 6 leaves per plantlet. Key words: Protocorn Like Bodies; tissue culture; V&W medium; auxins; cytokinins.

scholarly journals PROPAGASI IN VITRO ANGGREK (Dendrobium phalaenopsis Fitzg) TERHADAP PEMBERIAN HORMON IBA DAN KINETIN

2017 ◽  
Vol 7 (1) ◽  
pp. 15
Author(s):  
IMAM MAHADI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Ornamental Plants ◽  
Special Treatment ◽  
Culture Techniques ◽  
Tissue Culture Techniques ◽  
Number Of Shoots

Orchids are a very popular ornamental plants, but orchids have a very slow vegetative growth, thus requiring special treatment in the grown.To  spur grow than development can be used through tissue culture techniques of multiplication. But the success of the technique of tissue culture is inseparable with give a substance on the plant growth regulator. This study aims to determine the growth  an influence  D.phalaenopsis Fitzg orchids of giving a gaint swith IBA administration and Kinetin according to in vitro  and as well as to obtain a concentration combination of  IBA and Kinetin on the best orchid D.Phalaenops is Fitzg explants.  Base don’t he results of the study indicate that the combination of IBA and Kinetin concentration giving a influence percentage of live explants,   number of shoots and number of roots orchid D. phalaenopsis Fitzg explants.Combination treatment of IBA 1.0 ppm and 0.1ppm Kinetin is a combination of the best treatment to produce a number of shoots orchids.The giving of IBA 0.5ppm in the medium without the addition of kinetin concentration can increase the number of roots of the orchid D.phalaenopsis Fitzg.

Effects of Different Plant Growth Regulator on in vitro Shoot Multiplication of Chrysanthemum morifolium

2003 ◽  
Vol 3 (6) ◽  
pp. 553-560 ◽  
Author(s):  
M.Z. Karim . ◽  
M.N. Amin . ◽  
M.A.K. Azad . ◽  
F. Begum . ◽  
M.M. Rahman . ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Shoot Multiplication ◽  
Chrysanthemum Morifolium

Influence of plant growth regulator upon in vitro propagation of Paulownia sp

2015 ◽  
Vol 208 ◽  
pp. S113
Author(s):  
Marcel Danci ◽  
Ioana Cretu ◽  
Giancarla Velicevici ◽  
Roxana Luca ◽  
Alina Simina ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
In Vitro Propagation

Shiraz Wines Made from Grape Berries (Vitis vinifera) Delayed in Ripening by Plant Growth Regulator Treatment Have Elevated Rotundone Concentrations and “Pepper” Flavor and Aroma

2015 ◽  
Vol 63 (8) ◽  
pp. 2137-2144 ◽  
Author(s):  
Christopher Davies ◽  
Emily L. Nicholson ◽  
Christine Böttcher ◽  
Crista A. Burbidge ◽  
Susan E. P. Bastian ◽  
...  
Keyword(s):  
Plant Growth ◽  
Vitis Vinifera ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Grape Berries ◽  
Growth Regulator Treatment

scholarly journals In vitro plantlet production of the endangered Pinguicula vulgaris

2012 ◽  
Vol 7 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Tomás Grevenstuk ◽  
Anabela Romano
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Normal Development ◽  
Shoot Proliferation ◽  
Carnivorous Plant ◽  
Habitat Requirements ◽  
Ex Vitro ◽  
Plantlet Production

AbstractThis study describes the development of a micropropagation protocol for Pinguicula vulgaris using cultures initiated from in vitro produced seedlings. P. vulgaris is a carnivorous plant with a northern, disjunctly circumpolar distribution and specific habitat requirements, and is hence becoming increasingly rare. Shoot proliferation was significantly influenced by Murashige and Skoog (MS) macronutrient concentration, showing higher proliferation rates in 1/4MS, but was not affected by the addition of 0.1 mg/L 6-benzyladenine (BA) or zeatin (Zea). The best medium for propagating P. vulgaris was plant growth regulator (PGR) free ¼MS. An average of 7.62 new shoots per initial explant could be obtained after 8 weeks of culture, of which over 79% produced roots during proliferation. Moreover, rooting percentages of 100% were obtained for the initial explants in all the tested media, including media without PGRs. The plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development.

scholarly journals USE OF FORCING SOLUTIONS TO STUDY PLANT GROWTH REGULATOR EFFECTS ON VANHOUTTE'S SPIRAEA CULTURED IN VITRO

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1124e-1124
Author(s):  
Guochen Yang ◽  
P. E. Read
Keyword(s):  
Plant Physiology ◽  
Plant Growth ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Woody Plant ◽  
Shoot Proliferation ◽  
Plant Tissues ◽  
Forcing Solution

Vanhoutte's spiraea has been propagated in vitro using explants from softwood growth of dormant stems forced in a solution containing 200 mg/l 8-hydroxyquinoline citrate (8-HQC) and 2% sucrose (Yang and Read, 1989). Objectives to further utilize this system were to determine the feasibility of applying plant growth regulators (PGR) via the forcing solution to softwood growth from forced dormant stems and to study the resulting influence on in vitro culture. BA and GA3 were placed in the forcing solution at various concentrations, including a zero PGR control. Explants were cultured on Linsmaier and Skoog (LS) medium containing zero PGR or different amounts of BA or thidiazuron (TDZ) or combinations of BA and IAA. Control explants placed on LS medium supplemented with 5uM BA with or without 1 or 5uM IAA, or with 0.5 or 0.75 uM TDZ alone produced the best shoot proliferation. BA in the forcing solution stimulated micropropagation, while GA3 caused less proliferation than explants from control solutions. Forcing solutions containing PGR are useful for manipulating responses of plant tissues cultured in vitro and for studying PGR influence on woody plant physiology.

scholarly journals Effects of explant type, plant growth regulator and cultivation system on multiplication of Polygonum multiflora Thunb. cultured in vitro

2020 ◽  
Vol 17 (4) ◽  
pp. 689-698
Author(s):  
Vu Quoc Luan ◽  
Do Thi Luyen ◽  
Ho Hoang Anh Kha ◽  
Hoang Thanh Tung ◽  
Vu Thi Hien ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Explant Type ◽  
Cultivation System
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