Analisis Kimia Ikan Ekor Kuning Asap Industri Rumah Tangga di Kota Jayapura

Fish is highly susceptible to deterioration without any preservative or processing measures and requires proper handling and preservation to increase its shelf life. Smoked fish is traditionally processed by fish through an open process of hot smoked. Smoked fish process in Indonesia, especially in Papua, is still carried out traditionally with capital and small business scale so that the use of tools is still simple. The aim of this research was to study the proximate composition of smoked yellowfin fish, which were taken from different producers in Youtefa Market, Jayapura City, Papua. The analysis on smoked yellowfin fish applied of proximate analysis (moisture level, ash level, fat level, and protein level). The result of this research showed that the highest moisture level was EK.K sample with 67.17%. The highest value of ash level was EK.T­1 sample with 8.15%. The highest of fat and protein level were EK.K sample, with 18.63% and 6.42%, respectively. Key words: Smoked yellowfin, proximate analysis.

Pedoman Penulisan

Pedoman Penulisan Jurnal Biologi Papua

Pengelola Jurnal

Pengelola Jurnal Edisi Volume 13 Nomor 1 2021

Identifikasi Molekular Kekerabatan Genetik Kopi Wamena Berbasis Marka Random Amplified of Polymorphic DNA (RAPD)

Baliem Coffee or Wamena Arabica Coffee is classified as a specialty coffee that has a distinctive aroma and taste as one of the world's best coffees. Wamena coffee has a high commercial value, there are many advantages of Wamena coffee. Growing on a variety of soil types, elevation, coffee variety, and durable. The quality of Wamena coffee must be maintained by the local government. However, this elite coffee germplasm is threatened with extinction and is in a dangerous situation due to habitat destruction due to forest clearing, land use change and the potential for competition with various disease-resistant varieties. This study aims to identify molecularly the genetic relationship of Wamena Arabica coffee in Jayawijaya district with the Random Amplified Polymorphic DNA (RAPD) markers as molecular markers. The stages in this study were sample collection, morphological observations, genomic DNA extracted using the CTAB method, gene amplification process using PCR-RAPD molecular markers with ten primers, measurement of DNA concentration using a spectrophotometer, and data analysis using UPGMA NTSYS version 2.1. The electroferogram results showed that pRAPD1, pRAPD2 and pRAPD5 primers produced a band pattern with high polymorphism as well as indicating the genetic diversity of the Coffea arabica L. arabica coffee samples tested. Based on the dendogram construction analysis, the arabica coffee sample Coffea arabica L. which was tested on 5 coffee genotypes, obtained 2 coffee groups, namely Group 1 consisting of one variety, namely sample (V) Arabica Typica from the Assolokobal area. Group 2 with four varieties, namely samples (I) Arabica Catimor, (II) USDA, (III) Arabica PM 88, and (IV) Arabica Linies 795, from Wollo. Phylogenetic tree construction resulted in a similarity coefficient of 83%, indicating the high relationship between Arabica coffee populations. The results of this study have obtained a genetic fingerprint profile of wamena specialty arabica coffee, namely its genetic relationship. The superiority of the genetic composition of Wamena coffee in Jayawijaya district in the future has the potential to be derived through much more advanced molecular technology.

Pertumbuhan dan Perkembangan Protocorn Like Bodies (PLB) Anggrek Papua Dendrobium discolor dengan Perlakuan Auksin dan Sitokinin

Dendrobium could meet the demands of domestic and foreign consumers from time to time because of their very charming flower blossoms. Orchid flowers are attractive because of the variety their color, size, shape, composition, number of buds the blossom of flowers, especially for the color it is vary greatly. One of the potential Dendrobium Papua orchids is Dendrobium discolor. To fulfill the needs of Dendrobium orchids in pots or in peaces it is depend on the success of Protocorn Like Bodies (PLB) cultivation. The purpose of this study was to determine the growth and development of PLB cultivated in vitro on Vacint & Went (V&W) base media with a combination of different plant growth regulators. The method used wasPLB cultivation with tissue culture techniques in vitro on V&W media with a factorial completely randomized design (CRD). The first factor was auxin treatment (2,4D) with 5 concentrations 1, 2, 3, and 4 ppm plus control (without plant growth regulator treatment), while the second factor was cytokinin treatment with 5 concentrations 1, 2, 3, and 4 ppm plus 1 control (without plant growth regulator treatment). Each treatment repeated 5 times, threfore, there are 125 treatment units in total 125 treatment units. The media used were Vacin and Went (VW). The variables measured were leaf length and the number of leaves per plantlet in each combination treatment of plant growth regulator (PGR) after one month of cultivation. Data were analyzed by using variance (ANOVA) to determine the significance. The results of this study indicated that the combination treatment of auxin and cytokinin PGR in V&W media had a significant effect on the length and number of plantlets leaves and the most significant treatment was the combination of PGR auxin: cytokinin = 0:3 ppm. So it can be concluded that the best medium for PLB culture is a combination of PGR auxin: cytokinin = 0:3 ppm. with an average value of 3.5 cm plantlet leaf length and number of 6 leaves per plantlet. Key words: Protocorn Like Bodies; tissue culture; V&W medium; auxins; cytokinins.

Analisis Kromosom Varietas Kacang Gude (Cajanus cajan (L.) Millspaugh) Asal Pulau Kisar, Maluku Barat Daya (MBD)

Pigeon pea (Cajanus cajan) is one of the biodiversity of Kisar Island, Maluku Barat Daya (MBD). This study aimed to determine the number and size of chromosomes from pigeon pea (C. cajan) from Kisar Island, MBD. The method used for chromosome observation is the squashing method, while for the analysis of the shape and size of chromosomes using the picsart and image raster applications. Data analysis using descriptive analysis with the help of figures and tables. The results showed that the chromosome number of three varieties of pigeon pea (C. cajan) was 2n = 14. The longest chromosome is owned by orange seed varieties that are 9.17 µm, batik color seed varieties are 9.09 μm, the shortest chromosomes are owned by black seed varieties of 8.32 µm. The chromosome length of the three varieties of pigeon pea (C. cajan) varies showing different genome numbers. Key words: Chromosome; Cajanus cajan; Kisar Island; Maluku Barat Daya.

Pengembangan Produk Minuman Tonikum Kulit Kayu Akway (Drymis piperita): Prospek Penambah Stamina Tubuh Secara In Vivo

Akway bark (Drymis piperita) from Papua is widely used by local people in Arfak Mountains of West Papua to increase stamina. The purpose of this study was to determine the physical quality and tonic effects of Akway bark (bark tonic drink D. piperita) on mice (Mus musculus). The swimming resistance used to determine the tonic effect of mice (24 individuals) which divided into 6 groups. Group I was given water, group II tonic drink base, group III as positive control of 50 mg caffeine energy drinks, groups IV, V, and VI were given bark tonic drink 10mg /kg body weight, 30 mg/kg body weight, and 50mg /kg body weight respectively. The total period swimming until fatigue was measured and used as an index of swimming capacity. The results of physical quality of the Akway bark tonic drink has a sweet and sour taste, with clear dark brown color, had distinctive aroma with pH of 4, and its volume was 100 ml with solubility deposits on day 21. The results of pre-clinical testing shown the highest dose was 50 mg/ weight swimming time 9.28 minutes and having better stamina. The next was with dose of 30 mg/kgBW, swimming for for 6.01 minutes and the last was dose of 10 mg /kgBW swimming for 3.23 minutes. The results of the one-way ANOVA test showed that it was significantly different between the 10 mg/kgBW, 30 mg/kgBW and 50 mg/kgBW groups. Key words: drink; D. piperita tonic; effect; mice; Papua.

Persepsi Publik Terhadap Pengelolaan Lingkungan Hidup di Wilayah Jayapura–Papua

Papua has a huge diversity of natural resources which can be categorized as well managed compare to other areas in Indonesia. The city of Jayapura and its surrounding are arranged to become an urban area nowadays. As an impact, many sector has changing including its land use. This research aims to understanding the knowledge, perception, and assesment of people regarding the environment particularly in Jayapura and its environs. The research was conducted in Jayapura City, Jayapura Regency and Keerom Regency with sampling method using survey, questionnaire and documentation. This research revealed that 53.0–90.0% of respondent knowing about environmental management and most of them (90.0%) gained those information from educational institution such as schools. Regarding government participation in environmental management, respondent answered that it is around 69.0–76.0% while for infrastructure and monitoring it is around 52.4-74.0% and 41.0-70.6% respectively. Concerning the environmental condition and its management the rate was 6.20 which can be categoized as good.Key words: people perception; environment; government; Jayapura.

Struktur dan Komposisi Hutan Mangrove di Kampung Sakartemin Distrik Fakfak Tengah, Kabupaten Fakfak, Papua Barat

Sakartemin is one of the village Fakfak Regency which has naturally growing mangrove forest. However, due to the economic development, the government should build up some infrastructures near by those mangrove forest. The disturbance nearby mangrove ecosystem cause changes in structure and composition of mangrove vegetation. This study aims to identify the types of mangrove vegetation and to find out the structure and composition of mangrove forest in Sakartemin Village, Central Fakfak District, Fakfak Regency. The method that used in this study is vegetation survey method combining path and checkered line method. The result shown that mangrove species in the sakartemin village consist of 5 families, namely: Rhizophoraceae, Meliaceae, Soneratiaceae, Pandanaceae, and Myrsinaceae which consists of 7 species namely: Rhizophora stylosa, R. apiculata, Xylocarpus granatum, Soneratia alba, Bruguiera gymnhoriza, Pandanus sp., and Aegiceras corniculatum. At the seedling level found 1002 individuals, sapling level found 404 individuals, and tree level found 319 individuals. The highest importance value index (IVI) at seedling level was R. stylosa 55.41% and the lowest was A. corniculatum 9.49%. The highest IVI at sapling level was R. apiculata 57.72% and the lowest was Pandanus 5.49%. The highest IVI at the tree level was R. apiculata 88.62% and the lowest was B. gymnhoriza 42.45%.Key words: Sakartemin village; mangrove; structure; composition; Fakfak.

Kajian Tentang Hematologi Penderita Plasmodium vivax di Laboratorium Inti Farma Jayapura-Papua

Malaria due to Plasmodium vivax infection is a species that attacks humans more than other species. The purpose of this study was to determine the hematological description of Plasmodium vivax sufferers in the Inti Farma Jayapura Laboratory for the period January - June 2020. The descriptive study design used malaria examination using the peripheral blood smear method which was examined using a microscope at the Inti Farma Jayapura laboratory during the period January to June of the year. 2020. The results of the hematological examination were analyzed with descriptive statistics and crosstabulation using the SPSS version 25 program. The results revealed that the mean hemoglobin level of patients with vivax malaria was 12.12, the number of erythrocytes was 5.2750 million / miuL, the leukocyte count was 5,500 miuL, and the trombosiy was 303 thousand. / miuL. These findings provide important information for malaria management in general in Kota Jayapura.Key words: Laboratory, Plasmodium vivax, hematology, peripheral blood smear