scholarly journals Genetic diversity of multiple human immunodeficiency virus-1 (HIV-1) clades in West Papua

2021 ◽  
Vol 948 (1) ◽  
pp. 012077
Author(s):  
M Widiyanti ◽  
S Adiningsih ◽  
T N Kridaningsih
Keyword(s):  
Human Immunodeficiency Virus ◽  
Genetic Variability ◽  
Clinical Stage ◽  
Recombinant Form ◽  
Outlier Group ◽  
Immunodeficiency Virus ◽  
West Papua ◽  
Male Patients ◽  
Hiv Envelope ◽  
Hiv 1

Abstract Human Immunodeficiency Virus (HIV) genetic variability is classified into types, groups, and subtypes. HIV is divided into 2 types, HIV-1 and HIV-2. HIV-1 was divided into groups M (Main), N (New), and O (Outlier). Group M was divided into 9 subtypes and 48 Circulating Recombinant Form (CRF). Understanding HIV-1 subtype distribution and epidemiology can assist preventive measures and clinical decisions. Sequence variation may affect drug resistance, disease progression, and transmission route. This study aimed to determine human immunodeficiency virus type-1 (HIV-1) subtypes which circulate in West Papua based on gene fragments encoding the glycoprotein-41 (gp41) of the HIV envelope. The descriptive-analytic method was attended at the VCT clinic in West Papua. Samples of blood plasma from patients with HIV were amplified using RT-PCR and nested PCR. Genetic variability was analyzed using DNA sequencing. The HIV-1 subtype was determined for 35 samples which 15 were from female patients and 20 were from male patients. 3 subtypes were detected: Circulating Recombinant Form AE (CRF01_AE, n=21), B (n=12), and 52_01B (n=2). Subtypes AE predominated in HIV-1 acquired heterosexually 100 % and clinical stage level III. Subtypes AE strains of group M viruses predominated in West Papua in HIV transmitted by heterosexual contact.

scholarly journals Active and Selective Transcytosis of Cell-Free Human Immunodeficiency Virus through a Tight Polarized Monolayer of Human Endometrial Cells

2001 ◽  
Vol 75 (11) ◽  
pp. 5370-5374 ◽  
Author(s):  
Hakim Hocini ◽  
Pierre Becquart ◽  
Hicham Bouhlal ◽  
Nicolas Chomont ◽  
Petronela Ancuta ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Apical Membrane ◽  
Heterosexual Transmission ◽  
Free Form ◽  
Endometrial Cells ◽  
Immunodeficiency Virus ◽  
A Cell ◽  
Hiv Envelope ◽  
Hiv 1

ABSTRACT We report that both primary and laboratory-adapted infectious human immunodeficiency virus type 1 (HIV-1) isolates in a cell-free form are capable of transcytosis through a tight and polarized monolayer of human endometrial cells. Trancytosis of cell-free HIV occurs in a strain-selective fashion and appears to be dependent on interactions between HIV envelope glycoproteins and lectins on the apical membrane of the epithelial cells. These findings provide new insights into the initial events occurring during heterosexual transmission of the virus.

The subtypes of human immunodeficiency virus in Australia and Asia

Sexual Health ◽  
2004 ◽  
Vol 1 (1) ◽  
pp. 1 ◽  
Author(s):  
Robert Oelrichs
Keyword(s):  
Human Immunodeficiency Virus ◽  
Genetic Variability ◽  
Distribution Patterns ◽  
Human Populations ◽  
Regional Diversity ◽  
Subtype B ◽  
Immunodeficiency Virus ◽  
Australasian Region ◽  
Hiv 1

Worldwide, the human immunodeficiency virus exhibits a great genetic variability, with multiple circulating subtypes of the virus. This variability allows study of the movement of HIV strains within and between human populations but also has implications for diagnosis, treatment and monitoring. The type of HIV causing the epidemic in Australia is changing from being homogeneous subtype B, reflecting a greater regional diversity. In this paper the classification of HIV-1 subtypes and their distribution within the Australasian region are reviewed and the implications of these distribution patterns discussed.

scholarly journals A Conformational Switch in Human Immunodeficiency Virus gp41 Revealed by the Structures of Overlapping Epitopes Recognized by Neutralizing Antibodies

2009 ◽  
Vol 83 (17) ◽  
pp. 8451-8462 ◽  
Author(s):  
Robert Pejchal ◽  
Johannes S. Gach ◽  
Florence M. Brunel ◽  
Rosa M. Cardoso ◽  
Robyn L. Stanfield ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Neutralizing Antibodies ◽  
Helical Structure ◽  
Viral Fusion ◽  
Fab Fragment ◽  
Conformational Switch ◽  
Immunodeficiency Virus ◽  
Hiv Envelope ◽  
Hiv 1

ABSTRACT The membrane-proximal external region (MPER) of the human immunodeficiency virus (HIV) envelope glycoprotein (gp41) is critical for viral fusion and infectivity and is the target of three of the five known broadly neutralizing HIV type 1 (HIV-1) antibodies, 2F5, Z13, and 4E10. Here, we report the crystal structure of the Fab fragment of Z13e1, an affinity-enhanced variant of monoclonal antibody Z13, in complex with a 12-residue peptide corresponding to the core epitope (W670NWFDITN677) at 1.8-Å resolution. The bound peptide adopts an S-shaped conformation composed of two tandem, perpendicular helical turns. This conformation differs strikingly from the α-helical structure adopted by an overlapping MPER peptide bound to 4E10. Z13e1 binds to an elbow in the MPER at the membrane interface, making relatively few interactions with conserved aromatics (Trp672 and Phe673) that are critical for 4E10 recognition. The comparison of the Z13e1 and 4E10 epitope structures reveals a conformational switch such that neutralization can occur by the recognition of the different conformations and faces of the largely amphipathic MPER. The Z13e1 structure provides significant new insights into the dynamic nature of the MPER, which likely is critical for membrane fusion, and it has significant implications for mechanisms of HIV-1 neutralization by MPER antibodies and for the design of HIV-1 immunogens.

scholarly journals A Recent Outbreak of Human Immunodeficiency Virus Type 1 Infection in Southern China Was Initiated by Two Highly Homogeneous, Geographically Separated Strains, Circulating Recombinant Form AE and a Novel BC Recombinant

2000 ◽  
Vol 74 (23) ◽  
pp. 11286-11295 ◽  
Author(s):  
Sucheep Piyasirisilp ◽  
Francine E. McCutchan ◽  
Jean K. Carr ◽  
Eric Sanders-Buell ◽  
Wei Liu ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Southern China ◽  
Recombinant Form ◽  
Subtype C ◽  
Subtype B ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT New outbreaks of human immunodeficiency virus type 1 (HIV-1) among injecting drug users (IDUs) are spreading in China along heroin trafficking routes. Recently, two separate HIV-1 epidemics among IDUs were reported in Guangxi, Southern China, where partial sequencing of the env gene showed subtype C and circulating recombinant form (CRF) AE. We evaluated five virtually full-length HIV-1 genome sequences from IDUs in Guangxi to determine the genetic diversity and the presence of intersubtype recombinants. Sequence analysis showed two geographically separated, highly homogeneous HIV-1 strains. B/C intersubtype recombinants were found in three IDUs from Baise City, in a mountainous region near the Yunnan-Guangxi border. These were mostly subtype C, with portions of the capsid and reverse transcriptase (RT) genes from subtype B. The subtype B portion of the capsid was located in the N-terminal domain, which has been shown to influence virus core maturation, virus infectivity, and binding to cyclophilin A, whereas the subtype B portion of RT was located in the palm subdomain, which is the active site of the enzyme. These BC recombinants differed from a BC recombinant found in Xinjiang Province in northwestern China. CRF AE strains were found in IDUs from Nanning, the capital of Guangxi, and in IDUs from Pingxiang City near the China-Vietnam border. The AE and BC recombinants were both remarkable for their low interpatient diversity, less than 1% for the full genome. Rapid spread of HIV-1 among IDUs may foster the emergence of highly homogeneous strains, including novel recombinants in regions with multiple subtypes.

scholarly journals Changes in the Immunogenic Properties of Soluble gp140 Human Immunodeficiency Virus Envelope Constructs upon Partial Deletion of the Second Hypervariable Region

2003 ◽  
Vol 77 (4) ◽  
pp. 2310-2320 ◽  
Author(s):  
Indresh K. Srivastava ◽  
Keating VanDorsten ◽  
Lucia Vojtech ◽  
Susan W. Barnett ◽  
Leonidas Stamatatos
Keyword(s):  
Human Immunodeficiency Virus ◽  
Binding Site ◽  
Hypervariable Region ◽  
V3 Loop ◽  
Virus Envelope ◽  
Immunodeficiency Virus ◽  
Cd4 Binding Site ◽  
Hiv Envelope ◽  
Immunogenic Properties ◽  
Hiv 1

ABSTRACT Immunization of macaques with the soluble oligomeric gp140 form of the SF162 envelope (SF162gp140) or with an SF162gp140-derived construct lacking the central region of the V2 loop (ΔV2gp140) results in the generation of high titers of antibodies capable of neutralizing the homologous human immunodeficiency virus type 1 (HIV-1), SF162 virus (Barnett et al. J. Virol. 75 :5526-5540, 2001). However, the ΔV2gp140 immunogen is more effective than the SF162gp140 immunogen in eliciting the generation of antibodies capable of neutralizing heterologous HIV-1 isolates. This indicates that deletion of the V2 loop alters the immunogenicity of the SF162gp140 protein. The present studies were aimed at identifying the envelope regions whose immunogenicity is altered following V2 loop deletion. We report that the antibodies elicited by the SF162gp140 immunogen recognize elements of the V1, V2, and V3 loops, the CD4-binding site, and the C1 and C2 regions on the homologous SF162 gp120. With the exception of the V1 and V2 loops, the same regions are recognized on heterologous gp120 proteins. Surprisingly, although a minority of the SF162gp140-elicited antibodies target the V3 loop on the homologous gp120, the majority of the antibodies elicited by this immunogen that are capable of binding to the heterologous gp120s tested recognize their V3 loops. Deletion of the V2 loop has two effects. First, it alters the immunogenicity of the V3 and V1 loops, and second, it renders the C5 region immunogenic. Although deletion of the V2 loop does not result in an increase in the immunogenicity of the CD4-binding site per se, the relative ratio of anti-CD4-binding site to anti-V3 loop antibodies that bind to the heterologous gp120s tested is higher in sera collected from the ΔV2gp140-immunized animals than in the SF162gp140-immunized animals. Overall, our studies indicate that it is possible to alter the immunogenic structure of the HIV envelope by introducing specific modifications.

scholarly journals Replicating Rather than Nonreplicating Adenovirus-Human Immunodeficiency Virus Recombinant Vaccines Are Better at Eliciting Potent Cellular Immunity and Priming High-Titer Antibodies

2005 ◽  
Vol 79 (16) ◽  
pp. 10200-10209 ◽  
Author(s):  
Bo Peng ◽  
Liqun Rejean Wang ◽  
Victor Raúl Gómez-Román ◽  
Alberta Davis-Warren ◽  
David C. Montefiori ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Neutralizing Antibodies ◽  
High Titer ◽  
Neutralizing Antibody ◽  
Preclinical Trial ◽  
Immunodeficiency Virus ◽  
Hiv Envelope ◽  
Hiv 1

ABSTRACT A major challenge in combating the human immunodeficiency virus (HIV) epidemic is the development of vaccines capable of inducing potent, persistent cellular immunity and broadly reactive neutralizing antibody responses to HIV type 1 (HIV-1). We report here the results of a preclinical trial using the chimpanzee model to investigate a combination vaccine strategy involving sequential priming immunizations with different serotypes of adenovirus (Ad)/HIV-1MN env/rev recombinants and boosting with an HIV envelope subunit protein, oligomeric HIVSF162 gp140ΔV2. The immunogenicities of replicating and nonreplicating Ad/HIV-1MN env/rev recombinants were compared. Replicating Ad/HIV recombinants were better at eliciting HIV-specific cellular immune responses and better at priming humoral immunity against HIV than nonreplicating Ad-HIV recombinants carrying the same gene insert. Enhanced cellular immunity was manifested by a greater frequency of HIV envelope-specific gamma interferon-secreting peripheral blood lymphocytes and better priming of T-cell proliferative responses. Enhanced humoral immunity was seen in higher anti-envelope binding and neutralizing antibody titers and better induction of antibody-dependent cellular cytotoxicity. More animals primed with replicating Ad recombinants mounted neutralizing antibodies against heterologous R5 viruses after one or two booster immunizations with the mismatched oligomeric HIV-1SF162 gp140ΔV2 protein. These results support continued development of the replicating Ad-HIV recombinant vaccine approach and suggest that the use of replicating vectors for other vaccines may prove fruitful.

scholarly journals Dissociation of the CD4 and CXCR4 Binding Properties of Human Immunodeficiency Virus Type 1 gp120 by Deletion of the First Putative Alpha-Helical Conserved Structure

1998 ◽  
Vol 72 (9) ◽  
pp. 7280-7288 ◽  
Author(s):  
Dorothée Missé ◽  
Martine Cerutti ◽  
Isabelle Schmidt ◽  
Aline Jansen ◽  
Gérard Devauchelle ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Alpha Helix ◽  
Helical Structure ◽  
Natural Ligand ◽  
Immunodeficiency Virus ◽  
Hiv Envelope ◽  
Hiv 1

ABSTRACT To evaluate conserved structures of the surface gp120 subunit (SU) of the human immunodeficiency virus type 1 (HIV-1) envelope in gp120-cell interactions, we designed and produced an HIV-1 IIIB (HXB2R) gp120 carrying a deletion of amino acids E61 to S85. This sequence corresponds to a highly conserved predicted amphipathic alpha-helical structure located in the gp120 C1 region. The resultant soluble mutant with a deleted alpha helix 1 (gp120 ΔαHX1) exhibited a strong interaction with CXCR4, although CD4 binding was undetectable. The former interaction was specific since it inhibited the binding of the anti-CXCR4 monoclonal antibody (12G5), as well as SDF1α, the natural ligand of CXCR4. Additionally, the mutant gp120 was able to bind to CXCR4+/CD4− cells but not to CXCR4−/CD4− cells. Although efficiently expressed on cell surface, HIV envelope harboring the deleted gp120 ΔαHX1 associated with wild-type transmembrane gp41 was unable to induce cell-to-cell fusion with HeLa CD4+ cells. Nevertheless, the soluble gp120 ΔαHX1 efficiently inhibited a single round of HIV-1 LAI infection in HeLa P4 cells, with a 50% inhibitory concentration of 100 nM. Our data demonstrate that interaction with the CXCR4 coreceptor was maintained in a SUgp120 HIV envelope lacking αHX1. Moreover, in the absence of CD4 binding, the interaction of gp120 ΔαHX1 with CXCR4 was sufficient to inhibit HIV-1 infection.

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