scholarly journals Effects of Growth Medium on Extracellular Secretion of Human Epidermal Growth Factor in Escherichia coli by Co-expression with Bacillus cereus Phospholipase C

2020 ◽  
Vol 833 ◽  
pp. 012021
Author(s):  
F P U Latifah ◽  
A Indriyani ◽  
R D Pratiwi ◽  
Sriwidodo ◽  
IP Maksum
Keyword(s):  
Escherichia Coli ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Bacillus Cereus ◽  
Phospholipase C ◽  
Growth Medium ◽  
Human Epidermal Growth Factor ◽  
Extracellular Secretion ◽  
Epidermal Growth

scholarly journals Optimization extracellular secretion of Recombinant Human Epidermal Growth Factor (hEGF) in Escherichia coli BL21 (DE3) pD881-OmpA-hEGF by using Response Surface Method (RSM)

2019 ◽  
Vol 10 (3) ◽  
pp. 1824-1831 ◽  
Author(s):  
Annisa Indriyani ◽  
Nenden Indriyani Anggraeni ◽  
Sriwidodo ◽  
Iman Permana Maksum
Keyword(s):  
Escherichia Coli ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Recombinant Protein ◽  
Optimum Condition ◽  
Recombinant Dna ◽  
Sandwich Elisa ◽  
Human Epidermal Growth Factor ◽  
Extracellular Secretion ◽  
Epidermal Growth

Human Epidermal Growth Factor is techno-economically protein that has the function for stimulates a process of proliferation and cell differentiation. Because of that function, it can be a candidate for wound healing in ulcer diabetic. In the previous study, hEGF can extracellular secreted by OmpA signal peptide using recombinant DNA. The production method of recombinant hEGF will be extracellular secreted by Escherichia coli. However, the optimum secretion of OmpA has not been studied. Therefore the purpose of this study is to determine the optimum conditions using the RSM method. This research begins with a rejuvenation of culture, extracellular secretion, and optimization with the RSM method, followed by protein production at optimum condition. Secreted recombinant protein measured by Sandwich ELISA method and LOWRY. The result showed that the optimum condition was found in the medium concentration of 1.5x and induction time at 2 hours 10 minutes and the total protein yield of expression was 21.247 mg/mL with optimization percentage 2.96% and efficiency 50.56 % and recombinant protein hEGF concentration in fermentation media 416 μg/mL

scholarly journals Synthesis and secretion of human epidermal growth factor by Escherichia coli.

1985 ◽  
Vol 82 (21) ◽  
pp. 7212-7216 ◽  
Author(s):  
T. Oka ◽  
S. Sakamoto ◽  
K. Miyoshi ◽  
T. Fuwa ◽  
K. Yoda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Human Epidermal Growth Factor ◽  
Epidermal Growth

scholarly journals Efficacies of different secretion vectors for secretion of human epidermal growth factor by Escherichia coli.

1987 ◽  
Vol 51 (4) ◽  
pp. 1099-1104 ◽  
Author(s):  
Takanori OKA ◽  
Shin-ichiro SUMI ◽  
Toru FUWA ◽  
Koji YODA ◽  
Makari YAMASAKI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Human Epidermal Growth Factor ◽  
Epidermal Growth
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