Creation of an ultra-long depth of focus super-resolution longitudinally polarized beam with a ternary optical element

This paper presents an experimental study designed to test the performance of the light sword lens (LSL) with different pupil sizes. To do so, Snellen optotype images obtained by a monofocal lens either with or without an LSL, were compared. Images were obtained for three different pupil sizes at several target vergences. The correlation coefficient and through-focus curves were obtained and compared. The experimental results show differences in the contrast and the depth of focus with different pupil sizes using the monofocal lens without an LSL. In contrast, when using the monofocal lens in combination with the LSL, the quality of the images is similar for all pupils and target vergences used, with slight differences only in halos and contrast. Full Text: PDF ReferencesG. Mikula, Z. Jaroszewicz, A. Kolodziejczyk, K. Petelczyc, M. Sypek, G. P. Agrawal, "Imaging with extended focal depth by means of lenses with radial and angular modulation", Opt Express 15, 9184, (2007). CrossRef A. Kolodziejczyk, S. Bará, Z. Jaroszewicz, M. Sypek, "The Light Sword Optical Element—a New Diffraction Structure with Extended Depth of Focus", J. Mod Opt. 37, 1283, (1990). CrossRef K. Petelczyc et al, "Presbyopia compensation with a light sword optical element of a variable diameter", Photonics Lett. Pol. 1, 55 (2009). DirectLink A. Mira-Agudelo et al, "Compensation of Presbyopia With the Light Sword Lens", Invest Ophthalmol Vis Sci 57, 6871, (2016). CrossRef R.A. Fisher, Statistical Methods for Research Workers (New York, Hafner, 13th Ed., 1958) CrossRef

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Sharper fluorescent super-resolution spot generated by azimuthally polarized beam in STED microscopy

Optics Express ◽

10.1364/oe.20.017653 ◽

2012 ◽

Vol 20 (16) ◽

pp. 17653 ◽

Cited By ~ 52

Author(s):

Yi Xue ◽

Cuifang Kuang ◽

Shuai Li ◽

Zhaotai Gu ◽

Xu Liu

Keyword(s):

Super Resolution ◽

Sted Microscopy ◽

Polarized Beam ◽

Azimuthally Polarized Beam

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High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism

Nanophotonics ◽

10.1515/nanoph-2019-0346 ◽

2019 ◽

Vol 9 (1) ◽

pp. 143-148

Author(s):

Adrien Descloux ◽

Marcel Müller ◽

Vytautas Navikas ◽

Andreas Markwirth ◽

Robin van den Eynde ◽

...

Keyword(s):

High Speed ◽

Three Dimensional ◽

Super Resolution ◽

Optical Element ◽

Spatial Light Modulators ◽

Structured Illumination ◽

Structured Illumination Microscopy ◽

Image Stack ◽

Light Modulators ◽

3D Information

AbstractSuper-resolution structured illumination microscopy (SR-SIM) can be conducted at video-rate acquisition speeds when combined with high-speed spatial light modulators and sCMOS cameras, rendering it particularly suitable for live-cell imaging. If, however, three-dimensional (3D) information is desired, the sequential acquisition of vertical image stacks employed by current setups significantly slows down the acquisition process. In this work, we present a multiplane approach to SR-SIM that overcomes this slowdown via the simultaneous acquisition of multiple object planes, employing a recently introduced multiplane image splitting prism combined with high-speed SIM illumination. This strategy requires only the introduction of a single optical element and the addition of a second camera to acquire a laterally highly resolved 3D image stack. We demonstrate the performance of multiplane SIM by applying this instrument to imaging the dynamics of mitochondria in living COS-7 cells.

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