A High-Resolution Polymerase Chain Reaction-Sequence-Specific Primer HLA-B*27 Typing Set and Its Application in Routine HLA-B27 Testing

2006 ◽  
Vol 10 (2) ◽  
pp. 98-103 ◽  
Author(s):  
J. Downing ◽  
E. Coates ◽  
J. Street ◽  
L. Hammond ◽  
T.J. Rees ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
High Resolution ◽  
Hla B27 ◽  
Specific Primer ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals HLA-B27 Determination by Polymerase Chain Reaction

1996 ◽  
Vol 12 (4) ◽  
pp. 247-251 ◽  
Author(s):  
D. C. Kilpatrick
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Samples ◽  
Hla B27 ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Laboratory Staff ◽  
Perfect Correlation ◽  
Serological Typing ◽  
Hla Type ◽  
Polymerase Chain

A method for determining the presence or absence of HLA-827 by selective amplification of a region in the third exon of the HLA-B27 gene common to B*270 I to B*2705 inclusive, was evaluated. This polymerase chain reaction/sequence specific primer (PCR-SSP) method gave perfect correlation with serological typing on 40 individuals of previously determined HLA type and on 50 further clinical samples elaluated blind. It was concluded that HLA-B27 determination by PCR-SSP is simple, reliable. cost-effectile and convient for laboratory staff.

Identification of a novel HLA-DRB1*04:94N allele by polymerase chain reaction sequence-based typing

2011 ◽  
Vol 78 (3) ◽  
pp. 226-227 ◽  
Author(s):  
F.-M. Zhu ◽  
W. Wang ◽  
W. Zhang ◽  
H.-J. Lv ◽  
L.-X. Yan
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Extração de DNA a partir de sangue humano coagulado para aplicação nas técnicas de genotipagem de antígenos leucocitários humanos e de receptores semelhantes à imunoglobulina

2009 ◽  
Vol 42 (6) ◽  
pp. 651-656 ◽  
Author(s):  
Daniela Maira Cardozo ◽  
Gláucia Andréia Guelsin ◽  
Samaia Laface Clementino ◽  
Fabiano Cavalcante de Melo ◽  
Marco Antônio Braga ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Killer Cell ◽  
Human Leukocyte ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Specific Sequence ◽  
Salting Out ◽  
Leukocyte Antigens ◽  
Polymerase Chain ◽  
Made In

O objetivo deste estudo foi padronizar uma metodologia de extração de DNA de alta qualidade a partir de amostras de sangue coagulado. Quarenta e oito amostras de sangue humano coagulado foram utilizadas para a extração de DNA pelo kit comercial EZ-DNA® (Biological Industries, Beit Haemek, Israel), pelo kit de coluna Neoscience® (One Lambda Inc., San Diego, CA) e pelo método modificado de salting out. Apenas o método de salting out foi capaz de extrair altas concentrações de DNA (média, 180ng/µL), as quais foram medidas pelo detector de fluorescência Qubit® (Invitrogen, USA). Este método permitiu a amplificação dos genes HLA (human leukocyte antigens) pela tecnologia PCR-SSO (polymerase chain reaction - specific sequence of oligonucleotides) Luminex, a qual exige DNA de boa qualidade, e de genes KIR (killer cell immunoglobulin-like receptors) pela técnica made in house PCR-SSP (polymerase chain reaction-sequence specific of primers), a qual demanda uma concentração específica de DNA (10ng/µL). Concluímos que a técnica de salting out modificada foi muito eficiente, simples e rápida para a extração de DNA de amostras de sangue humano coagulado, com o objetivo de realizar a genotipagem de genes HLA e KIR.

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