Size Exclusion Chromatography with Dual Wavelength Detection as a Sensitive and Accurate Method for Determining the Empty and Full Capsids of Recombinant Adeno-Associated Viral Vectors

2021 ◽  
Author(s):  
He Meng ◽  
Michelle Sorrentino ◽  
Denise Woodcock ◽  
Catherine R O'Riordan ◽  
Vijender Dhawan ◽  
...  
Keyword(s):  
Size Exclusion Chromatography ◽  
Viral Vectors ◽  
Accurate Method ◽  
Dual Wavelength ◽  
Size Exclusion ◽  
Exclusion Chromatography ◽  
Wavelength Detection

Polystyrene in N-Methylpyrrolidone: Intrinsic Viscosity-Molar Weight Relations and Polystyrene Unperturbed Dimensions

1993 ◽  
Vol 58 (10) ◽  
pp. 2396-2402
Author(s):  
Martin Konáš ◽  
Allan R. Shultz
Keyword(s):  
Intrinsic Viscosity ◽  
Size Exclusion Chromatography ◽  
Accurate Method ◽  
Size Exclusion ◽  
Unperturbed Dimensions ◽  
Molar Weight ◽  
Exclusion Chromatography ◽  
Weight Data

Intrinsic viscosity-molar weight relations are reported for polystyrene in N-methylpyrrolidine (NMP) and NMP/LiBr solutions at 30, 45, 60, 75, 90 and 105 °C. Also, Mark-Houwink constants KΘ and the unperturbed dimensions of the polystyrene chains at these temperatures are calculated by applying Stockmayer-Fixman extrapolations of the viscosity/molar weight data. The use of in-line size exclusion chromatography and viscometry is shown to be a facile and accurate method for such studies.

Sign in / Sign up

Export Citation Format

Share Document