Development of a Colloidal Gold Immunochromatographic Strip Assay for Rapid Detection of Bovine Rotavirus

2019 ◽  
Vol 32 (9) ◽  
pp. 393-401
Author(s):  
Zhenxue Li ◽  
Feipeng Zhao ◽  
Tingting Tang ◽  
Mengmeng Wang ◽  
Xiaoli Yu ◽  
...  

2021 ◽  
Author(s):  
beijun ang ◽  
Xin-Xin Xu ◽  
Liqiang Liu ◽  
Liguang Xu ◽  
Hua Kuang ◽  
...  

Shigella is the most common foodborne pathogen and can cause bacillary dysentery. Therefore, it is necessary to detect Shigella rapidly, accurately and to process large sample numbers, but it is...



2020 ◽  
Vol 99 (10) ◽  
pp. 4741-4749
Author(s):  
Wenlong Han ◽  
Zongchao Chen ◽  
Pengfei Niu ◽  
Xiaomei Ren ◽  
Chan Ding ◽  
...  








2021 ◽  
pp. 104063872110275
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Linfang Cheng ◽  
Hangping Yao ◽  
...  

Avian influenza A(H5) viruses (avian IAVs) pose a major threat to the economy and public health. We developed an antigen-ELISA (ag-ELISA) and a colloidal gold–based immunochromatographic strip for the rapid detection of avian A(H5) viruses. Both detection methods displayed no cross-reactivity with other viruses (e.g., other avian IAVs, infectious bursal disease virus, Newcastle disease virus, infectious bronchitis virus, avian paramyxovirus). The ag-ELISA was sensitive down to 0.5 hemagglutinin (HA) units/100 µL of avian A(H5) viruses and 7.5 ng/mL of purified H5 HA proteins. The immunochromatographic strip was sensitive down to 1 HA unit/100 µL of avian A(H5) viruses. Both detection methods exhibited good reproducibility with CVs < 10%. For 200 random poultry samples, the sensitivity and specificity of the ag-ELISA were 92.6% and 98.8%, respectively, and for test strips were 88.9% and 98.3%, respectively. Both detection methods displayed high specificity, sensitivity, and stability, making them suitable for rapid detection and field investigation of avian A(H5) viruses.



2018 ◽  
Vol 9 ◽  
Author(s):  
Zhihao Sun ◽  
Baolan Shi ◽  
Feifei Meng ◽  
Ruonan Ma ◽  
Qingyun Hu ◽  
...  


PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e92034 ◽  
Author(s):  
Ying Wang ◽  
Lifang Wang ◽  
Jianwei Zhang ◽  
Guangxi Wang ◽  
Wenbi Chen ◽  
...  


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