scholarly journals Antimicrobial Electrospun Polycaprolactone-Based Wound Dressings: An In Vitro Study About the Importance of the Direct Contact to Elicit Bactericidal Activity

2019 ◽  
Vol 8 (9) ◽  
pp. 438-451 ◽  
Author(s):  
Enrique Gámez ◽  
Gracia Mendoza ◽  
Sofía Salido ◽  
Manuel Arruebo ◽  
Silvia Irusta
Keyword(s):  
Bactericidal Activity ◽  
Direct Contact ◽  
In Vitro Study ◽  
Vitro Study ◽  
Wound Dressings

scholarly journals BactericidalActivity of Crevicular Polymorphonuclear Neutrophils in Chronic Periodontitis Patients and Healthy Subjects under the Influence of Areca Nut Extract: An In Vitro Study

2020 ◽  
Vol 10 (14) ◽  
pp. 5008
Author(s):  
Deepti Shrivastava ◽  
Kumar Chandan Srivastava ◽  
Jithendra Kajoor Dayakara ◽  
Mohammed G. Sghaireen ◽  
Ravi Kumar Gudipaneni ◽  
...  
Keyword(s):  
Bactericidal Activity ◽  
Chronic Periodontitis ◽  
Healthy Subjects ◽  
In Vitro Study ◽  
Study Groups ◽  
Vitro Study ◽  
Polymorphonuclear Neutrophils ◽  
H2o2 Production ◽  
Areca Nut

Arecanutchewing is an established risk factor for oral submucous fibrosis (OSMF), but its role in periodontal disease has not yet been defined. Thisstudy aimed to assess the effect of areca nut extracts (ANE) on the bactericidal activity of crevicular polymorphonuclear neutrophils (cPMNs) in healthy subjects and chronic periodontitis (CP) patients. An in vitro study was designed with an equal number of (n = 30) gingival crevicular fluid (GCF) samples collected from CP patients and healthy subjects. Bactericidal activity and hydrogen peroxide (H2O2) assays were performed with the GCF samples pre-treated with extracts of two varieties of areca nut: ripe and tender. Simultaneously, controls were also carried out with Hank’s balanced salt solution (HBSS) and catechin. Independent t-test and one-way analysis of variance (ANOVA), along with post-hoc analysis, were employed for statistical analysis. In both study groups, a significant reduction (p < 0.01)in the bactericidal activity was noted when the samples treated with the ripe areca nut (rANE) were compared with the tender variant (tANE). Similarly, H2O2 levels were significantly reduced (p < 0.001) in the rANE in contrast to tANE for both study groups. The above results were significant within the group but were found to be non-significant between the study groups, except when it was treated with HBSS (p < 0.001). In the present study, it was found that there was a reduction in the bactericidal activity and H2O2 production of cPMNs in both healthy subjects and CP patients in the presence of areca nut extract. Moreover, the effect of rANE on cPMNs was more detrimental than tANE.

Physiochemical and bactericidal activity evaluation: Silver‐augmented 3D‐ printed scaffolds—An in vitro study

2021 ◽  
Author(s):  
Vasudev Vivekanand Nayak ◽  
Nick Tovar ◽  
Jacques Henri Hacquebord ◽  
Simone Duarte ◽  
Beatriz H. D. Panariello ◽  
...  
Keyword(s):  
Bactericidal Activity ◽  
In Vitro Study ◽  
Vitro Study ◽  
Activity Evaluation ◽  
3D Printed

scholarly journals Evaluation of Antimicrobial Efficacy of Pudina (Menthaarvenis), Tulsi (Ocimumtenuiflorum) and Curry Leaves (Murrayakoenigii) on Streptococcus mutans by Modified Direct Contact Test- An In vitro Study

2020 ◽  
Author(s):  
Hima Patel ◽  
Adish Anand Saraf ◽  
TM Mangala ◽  
Rushikesh Mahaparale ◽  
Sneha Mali ◽  
...  
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Direct Contact ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antimicrobial Efficacy ◽  
Contact Test ◽  
Curry Leaves ◽  
Direct Contact Test

Introduction: Dental caries is a pandemic disease caused by Streptococcus mutans. Numerous preventive strategies have been developed to prevent dental caries with modern medicines. Recently, there has been a shift from use of modern medicines to herbal ayurvedic preparations which are easily available and cause least possible side effects but have not been tested against S. mutans. Aim: To evaluate antimicrobial value of Pudina, Tulsi and Curry leaves against S. mutans by modified direct contact test. Materials and Methods: This in vitro study used finely powdered form of Pudina, Tulsi and Curry leaves extracts (Hakim Chi Chi Pharmacy, Surat, India) without any added preservatives. The test microorganism S. mutans was isolated from human saliva using mitis salivarius agar. The Minimum Inhibition Concentration (MIC) was determined using agar well diffusion for Group 1 (Tulsi), Group 2 (Pudina), Group 3 (Curry leaves) and Group 4 (Chlorhexidine). Using this MIC, modified Direct Contact Test was performed for specific evaluation of the antimicrobial efficacy of each product. Chlorhexidine (0.2%) was used as a positive control for the comparison of each product. The results of modified direct contact test were subjected to ANOVA Test and Tukey’s Test using SPSS software version 23.0 which showed that when compared against chlorhexidine, the antimicrobial efficacy against S. mutans in decreasing order was of curry leaves, tulsi and pudina and the result of the study was significant. Results: Curry leaves showed higher antimicrobial efficacy than tulsi and pudina when compared against chlorhexidine (control) and the results were statistically significant (p<0.05). Conclusion: Curry leaf extract shows promising antimicrobial property against S. mutans and can be recommended for caries control with further investigations.

Chitosan-based hydrogel wound dressings with electrochemically incorporated silver nanoparticles – In vitro study

2019 ◽  
Vol 121 ◽  
pp. 109257 ◽  
Author(s):  
Katarina Nešović ◽  
Ana Janković ◽  
Tamara Radetić ◽  
Maja Vukašinović-Sekulić ◽  
Vesna Kojić ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
In Vitro Study ◽  
Vitro Study ◽  
Wound Dressings
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