scholarly journals Nucleocytoplasmic shuttling of Gle1 impacts DDX1 at transcription termination sites

2020 ◽  
Vol 31 (21) ◽  
pp. 2398-2408
Author(s):  
Manisha Sharma ◽  
Susan R. Wente
Keyword(s):  
Transcription Termination ◽  
Nucleocytoplasmic Shuttling ◽  
Mrna Cleavage ◽  
Mrna Transcripts ◽  
Nascent Mrna

We uncovered a nuclear role for human Gle1 in coordinating transcription termination. When nucleocytoplasmic shuttling of Gle1 is disrupted, nascent mRNA transcripts are elongated. Gle1 colocalizes with DDX1, and loss of Gle1 shuttling impairs recruitment of DDX1 to CstF-64 and transcription termination foci, leading to improper pre-mRNA cleavage.

scholarly journals Functional interaction between the RNA exosome and the sirtuin deacetylase Hst3 maintains transcriptional homeostasis

2021 ◽  
Author(s):  
Alysia R. Bryll ◽  
Craig L. Peterson
Keyword(s):  
Rna Synthesis ◽  
Optimal Level ◽  
Rna Degradation ◽  
Eukaryotic Cells ◽  
Functional Interaction ◽  
Mrna Transcripts ◽  
Rna Exosome ◽  
Nascent Mrna ◽  
Synthesis And Degradation ◽  
Global Reduction

Eukaryotic cells maintain an optimal level of mRNAs through unknown mechanisms that balance RNA synthesis and degradation. We found that inactivation of the RNA exosome leads to global reduction of nascent mRNA transcripts, and that this defect is accentuated by loss of deposition of histone variant H2A.Z. We identify the mRNA for the sirtuin deacetylase Hst3 as a key target for the RNA exosome that mediates communication between RNA degradation and transcription machineries. These findings reveal how the RNA exosome and H2A.Z function together to control a deacetylase, ensuring proper levels of transcription in response to changes in RNA degradation.

scholarly journals The Essential N Terminus of the Pta1 Scaffold Protein Is Required for snoRNA Transcription Termination and Ssu72 Function but Is Dispensable for Pre-mRNA 3′-End Processing

2009 ◽  
Vol 29 (8) ◽  
pp. 2296-2307 ◽  
Author(s):  
Mohamed A. Ghazy ◽  
Xiaoyuan He ◽  
Badri Nath Singh ◽  
Michael Hampsey ◽  
Claire Moore
Keyword(s):  
Amino Acids ◽  
Rna Polymerase Ii ◽  
Transcription Termination ◽  
Inhibitory Effect ◽  
Mrna Cleavage ◽  
Cleavage And Polyadenylation ◽  
Essential Region ◽  
Polyadenylation Factor

ABSTRACT Saccharomyces cerevisiae Pta1 is a component of the cleavage/polyadenylation factor (CPF) 3′-end processing complex and functions in pre-mRNA cleavage, poly(A) addition, and transcription termination. In this study, we investigated the role of the N-terminal region of Pta1 in transcription and processing. We report that a deletion of the first 75 amino acids (pta1-Δ75) causes thermosensitive growth, while the deletion of an additional 25 amino acids is lethal. The pta1-Δ75 mutant is defective for snoRNA termination, RNA polymerase II C-terminal domain Ser5-P dephosphorylation, and gene looping but is fully functional for mRNA 3′-end processing. Furthermore, different regions of Pta1 interact with the CPF subunits Ssu72, Pti1, and Ysh1, supporting the idea that Pta1 acts as a scaffold to organize CPF. The first 300 amino acids of Pta1 are sufficient for interactions with Ssu72, which is needed for pre-mRNA cleavage. By the degron-mediated depletion of Pta1, we show that the removal of this essential region leads to a loss of Ssu72, yet surprisingly, in vitro cleavage and polyadenylation remain efficient. In addition, a fragment containing amino acids 1 to 300 suppresses 3′-end processing in wild-type extracts. These findings suggest that the amino terminus of Pta1 has an inhibitory effect and that this effect can be neutralized through the interaction with Ssu72.

scholarly journals The INO80 remodeler couples premature termination of mRNA synthesis with transcription elongation

2020 ◽  
Author(s):  
Sara Luzzi ◽  
Ugo Szachnowski ◽  
Sarah Greener ◽  
Camille Gautier ◽  
Kang Hoo Han ◽  
...  
Keyword(s):  
Quality Control ◽  
Rna Polymerase Ii ◽  
Premature Termination ◽  
Transcription Termination ◽  
Transcriptional Elongation ◽  
Rna Quality ◽  
Mrna Transcripts ◽  
Rna Quality Control ◽  
Transcriptional Pausing ◽  
Elongation Process

SUMMARYRNA quality control and timely termination of aberrant transcription are critical for functional gene expression. Here, we report that in Saccharomyces cerevisiae premature transcription termination of mRNAs is coordinated with the transcriptional elongation process and regulated by the evolutionarily conserved ATP-dependent chromatin remodeling complex INO80. Loss of INO80 sensitizes cells to the transcriptional elongation stress drug 6-azauracil and leads to enhanced pausing of elongating RNA Polymerase II across the genome. Transcriptional pausing positively correlates with premature termination of mRNA transcription and is pronounced proximally to promoters at sites of enhanced histone H3 binding to DNA. Cells with deficient INO80 complex accumulate short, unproductive mRNA transcripts on chromatin and are defective in transcription termination mediated by the Nrd1-Nab3-Sen1 (NNS) complex. We find that loss of INO80 compromises the interaction of the RNA surveillance factor Nab2 with short promoter-proximal mRNA transcripts. INO80 promotes co-transcriptional recruitment of Nab2 to chromatin by enabling its interaction with the histone variant H2A.Z. Finally, inactivation of the histone deacetylase complex Rpd3S/Rco1 reduces promoter-proximal pausing and enhances productive transcription through an NNS-dependent termination site when INO80 is compromised. Our work suggests that, by regulation of H2A.Z-containing nucleosomes, INO80 orchestrates a mechanism for premature transcription termination, linking RNA quality control to the transcriptional process.

Sign in / Sign up

Export Citation Format

Share Document