NMR-based metabolomic analysis for the effects of creatine supplementation on mouse myoblast cell line C2C12

Creatine kinase (CK) plays an important role in buffering ATP and ADP levels in tissues which have intermittently high and fluctuating energy demands, such as skeletal muscle. This buffering function has a spatial, as well as a temporal aspect, which is dependent on the localization of different enzyme isoforms within the cell. We show here, by in situ hybridization, that the mRNAs for the cytoplasmic isoforms of CK are differentially localized in a mouse myoblast cell line (C2C12). The mRNA for the M form is localized at the cell periphery, while that for the B form is localized in the perinuclear region. Deletion of segments of the 3′ untranslated regions of these mRNAs or swapping of these segments between the mRNAs for the two isoforms demonstrated that localization signals lie within these regions. Localization appears to be tissue-specific, since both the M and B mRNAs were distributed uniformly over the cytoplasm in a non-muscle cell line. These results, in conjunction with other studies which have shown that mRNA localization can lead to co-localization of the encoded protein, suggest that the localization of the mRNAs for the cytoplasmic isoforms of CK may be involved in the localization of the enzymes themselves.

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Control of intracellular creatine concentration in a mouse myoblast cell line

Biochemical Society Transactions ◽

10.1042/bst021441s ◽

1993 ◽

Vol 21 (4) ◽

pp. 441S-441S ◽

Cited By ~ 8

Author(s):

J. E. ODOOM ◽

G. J. KEMP ◽

G. K. RADDA

Keyword(s):

Cell Line ◽

Myoblast Cell Line ◽

Myoblast Cell ◽

Mouse Myoblast

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Zebrafish Melanophores Suggest Novel Functions of Cell Chirality in Tissue Formation

Symmetry ◽

10.3390/sym13010130 ◽

2021 ◽

Vol 13 (1) ◽

pp. 130

Author(s):

Hiroaki Yamanaka ◽

Shigeru Kondo

Keyword(s):

Cell Line ◽

C2c12 Cells ◽

Tissue Formation ◽

Spiral Pattern ◽

Leftward Bias ◽

Asymmetric Behavior ◽

Myoblast Cell Line ◽

Culture Environment ◽

Myoblast Cell ◽

Mouse Myoblast

Several types of cells show left–right asymmetric behavior, unidirectional rotation, or spiral movements. For example, neutrophil-like differentiated HL60 (dHL60) cells show leftward bias in response to chemoattractant. Neurons extend neurites, creating a clockwise spiral. Platelet cells shows unidirectional spiral arrangements of actin fibers. In the microfabricated culture environment, groups of C2C12 cells (mouse myoblast cell line) were autonomously aligned in a counter-clockwise spiral pattern, and isolated C2C12 cells showed unidirectional spiral pattern of the actin skeleton. This biased directionality suggested that these cells have inherent cell chirality. In addition to these cells, we recently found that melanophores of zebrafish also have an intrinsic cellular chirality that was shown by their counter-clockwise self-rotation. Although this cell chirality is obvious, the function of the cell chirality is still unclear. In this review, we compare the cell chirality of melanophores of zebrafish with other cell chirality and consider the function of cell chirality in morphogenesis.

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