Effect of Calcium Ion on the Kolmer Complement-Fixation Test

1954 ◽  
Vol 24 (8) ◽  
pp. 934-945 ◽  
Author(s):  
Alcor S. Browne ◽  
Martha M. Michelbacher ◽  
Edith M. Coffey
1952 ◽  
Vol 21 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Elwood Buchman ◽  
Harold J. Kullman ◽  
George F. Margonis

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S113-S133 ◽  
Author(s):  
Sam Brody

ABSTRACT This report is a summary of 10 years of experience with the complement fixation test as adopted for the immunoassay of HCG in serum. It is based on published as well as unpublished material. The discussion centers mainly around methodological problems, criteria of reliability, and clinical observations. It is our impression that the complement fixation test is a reasonably rapid and simple technical procedure. It is standard practice in every bacteriological and virological laboratory. The precision of the HCG assay is high. Its accuracy is good. The complement fixation assay, as reported here, fulfils the criteria of specificity. It has been evaluated by means of serological techniques and through comparison between biopotency and immunopotency of HCG in serum with reference to a common standard. Its application for routine as well as research work is illustrated.


2014 ◽  
Vol 17 (2) ◽  
pp. 367-369 ◽  
Author(s):  
K. Rypula ◽  
A. Kumala ◽  
P. Lis ◽  
K. Niemczuk ◽  
K. Płoneczka-Janeczko ◽  
...  

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis


1984 ◽  
Vol 61 (7) ◽  
pp. 216-218
Author(s):  
L. C. LLOYD ◽  
R. T. BADMAN ◽  
J. R. ETHERIDGE ◽  
K. McKECHNIE ◽  
H. IYER

2001 ◽  
Vol 8 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini

ABSTRACT The efficacy of Brucella abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detectB. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovisalso reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.


1954 ◽  
Vol 52 (1) ◽  
pp. 87-99 ◽  
Author(s):  
J. B. Brooksby ◽  
Ella Wardle

A technique is presented for the titration of the virus of foot-and-mouth disease in culture in surviving epithelial tissue from the tongues of cattle. The cultures are incubated in cups on Perspex plates, and the detection of virus multiplication is by a complement-fixation test made on the culture in each cup.On the basis of comparative titrations in culture and in cattle, the method has been found to be as sensitive for the detection of virus as the titration by intradermal inoculation of the tongue of cattle. The method can also be applied in the detection of antibody in neutralization tests.We wish to record our thanks to Messrs E. Scoates and P. Mitchell for their technical assistance, and to Messrs H. M. Smith, R. H. Compton and R. L. Jackson for their part in the design and fabrication of various bottle rotators, plate shakers, Perspex lids and the inoculating box.


1968 ◽  
Vol 3 (1) ◽  
pp. 25-34 ◽  
Author(s):  
I. G. S. Furminger ◽  
A. J. Beale

Sign in / Sign up

Export Citation Format

Share Document