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Author(s):  
Shiny Joy ◽  
P. Eswara Prasad ◽  
K. Padmaja ◽  
K. Adilaxamamma ◽  
V. Chengalva Rayulu ◽  
...  

Background: A study was conducted to evaluate the antioxidant and immunological profile of methanolic extract of Adhatoda vasica leaves (AVE) in naturally infected sheep with gastrointestinal nematodes and compared with commonly used anthelmintic albendazole in sheep. Methods: Fifty four sheep found positive for gastrointestinal nematodes were divided into 3 equal groups, GI (infected-untreated: control), GII (infected- AVE treated) and GIII (infected- albendazole treated). Blood samples were drawn on 5th and 14th day after treatment for analysis of oxidative stress markers such as TBARS, TAC, SOD and catalase and immunological parameters such as total immunoglobulin, IgG and IL-10. Result: Helminthiasis in sheep cause a considerable oxidative stress. The anthelmintic activity of Adhatoda vasica surpassed the conventional synthetic drug albendazole in sheep in terms of oxidative and immunological changes in blood.


2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Jalal Aliabadi ◽  
Ehsan Rakhshandehroo ◽  
Azadeh Yektaseresht

Abstract Background The gastrointestinal helminth, Teladorsagia circumcincta, is one of the major health risks and production-limiting diseases in small ruminant populations, particularly in temperate regions. With the increasing importance of disease management and recruited anthelmintic resistant types, accurate approaches are needed for the diagnosis of the infection in the host. Due to uncertain results using faecal examinations, the ELISA method was indicated for the detection of nematode antigenic materials. Despite some promising results, problems were described in terms of test specificity and cross-reactions. Therefore, this study aimed to evaluate the IgG response to worm somatic and excretory/secretory (ES) products using western blot analysis and an indirect ELISA for the detection of T. circumcincta infection in sheep. Results Based on the immuno-reactivity analysis, immunogenic fractions with molecular weights (MWs) of approximately 60, 75 and 100 kDa were detected in somatic content and two antigens of about 63 and 75 kDa in ES material. Accordingly, a specific product at 75 kDa had the strongest reaction and appeared as the most common antigenic protein. In ELISA, all the sera from the infected sheep revealed the OD rates above the calculated cut-off value with about two-fold greater average. Negative control samples were also specifically recognized with the mean OD rate of about 1/3 of the estimated cut-off value. The cross-reaction test, using rabbit anti-T. circumcincta IgG, did not show reactivity with the ES antigens of other prevalent nematodes including Haemonchus contortus, Protostrongylus rufescens and Marshallagia marshalli. In contrast, a strong positive reaction was observed with the somatic antigens of M. marshalli. Conclusions The results of this study indicated that the indirect ELISA method using the ES content enables distinguishing the T. circumcincta infected sheep with high specificity. Those antigenic ES peptides with 63 and particularly 75 kDa MWs should be further investigated due to the potential for serological diagnostic methods and immunoprotective targets in the host.


2021 ◽  
Vol 12 ◽  
Author(s):  
Kavita Rawat ◽  
Aruna Pal ◽  
Samiddha Banerjee ◽  
Abantika Pal ◽  
Subhas Chandra Mandal ◽  
...  

CD14 (also known as the monocyte differentiation antigen) is an important immune response gene known to be primarily responsible for innate immunity against bacterial pathogens, and as a pattern recognition receptor (PRR), binds with LPS (endotoxin), lipoproteins, and lipotechoic acid of bacteria. So far very limited work has been conducted in parasitic immunology. In the current study, we reported the role of CD14 in parasitic immunology in livestock species (sheep) for the first time. Ovine CD14 is characterized as a horse-shoe shaped bent solenoid with a hydrophobic amino-terminal pocket for CD14 along with domains. High mutation frequency was observed, out of total 41 mutations identified, 23 mutations were observed to be thermodynamically unstable and 11 mutations were deleterious in nature, causing major functional alteration of important domains of CD14, an indication of variations in individual susceptibility for sheep against Haemonchus contortus infestations. In silico studies with molecular docking reveal a role of immune response against Haemonchus contortus in sheep, which is later confirmed with experimental evidence through differential mRNA expression analysis for sheep, which revealed better expression of CD14 in Haemonchus contortus infected sheep compared to that of non-infected sheep. We confirmed the above findings with supportive evidence through haematological and biochemical analyses. Phylogenetic analysis was conducted to assess the evolutionary relationship with respect to humans and it was observed that sheep may well be used as model organisms due to better genetic closeness compared to that of mice.


2021 ◽  
pp. 109524
Author(s):  
Tania Rojas-Campos ◽  
Froylán Ibarra-Velarde ◽  
Yolanda Vera-Montenegro ◽  
Miguel Flores-Ramos ◽  
Irene Cruz-Mendoza ◽  
...  

2021 ◽  
Author(s):  
Seyede Sogand Sajadi ◽  
Ali Haniloo ◽  
Samad Nadri ◽  
Negin Torabi

Abstract Echinococcus granulosus-developed metacestodes in the cultured medium are used for the assessment of its susceptibility to different compounds; however, this procedure is time-consuming and risky. In the present study, aspirated protoscoleces from the infected sheep were used to evaluate the effects of glucose, as an energy source, as well as ascorbic acid, as an antioxidant vitamin, on larval development. Protoscoleces were maintained in RPMI1640 culture media containing 10% fetal calf serum, as well as different concentrations of glucose (6 and 8 mg/ml) and ascorbic acid (25, 50, and 100 µg/ml). A culture medium containing 4 mg/ml of glucose was served as the control. Larger cysts were achieved in a shorter time from the medium enriched with 6 mg/ml of glucose (740 ± 20 µm) compared to the control group (420 ± 40 µm). However, in the groups treated with ascorbic acid, the number of cysts was higher in 100 µg/ml (32.5 ± 0.7) compared to the control group (12.5 ± 0.7). Additionally, the mature cysts were achieved on the 7th day of cultivation with 100 µg/ml of ascorbic acid compared to 18 days in the control group.


2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Emily Joanne Nixon ◽  
Ellen Brooks-Pollock ◽  
Richard Wall

Abstract Background Ovine psoroptic mange (sheep scab) is a highly pathogenic contagious infection caused by the mite Psoroptes ovis. Following 21 years in which scab was eradicated in the UK, it was inadvertently reintroduced in 1972 and, despite the implementation of a range of control methods, its prevalence increased steadily thereafter. Recent reports of resistance to macrocyclic lactone treatments may further exacerbate control problems. A better understanding of the factors that facilitate its transmission are required to allow improved management of this disease. Transmission of infection occurs within and between contiguous sheep farms via infected sheep-to-sheep or sheep–environment contact and through long-distance movements of infected sheep, such as through markets. Methods A stochastic metapopulation model was used to investigate the impact of different transmission routes on the spatial pattern of outbreaks. A range of model scenarios were considered following the initial infection of a cluster of highly connected contiguous farms. Results Scab spreads between clusters of neighbouring contiguous farms after introduction but when long-distance movements are excluded, infection then self-limits spatially at boundaries where farm connectivity is low. Inclusion of long-distance movements is required to generate the national patterns of disease spread observed. Conclusions Preventing the movement of scab infested sheep through sales and markets is essential for any national management programme. If effective movement control can be implemented, regional control in geographic areas where farm densities are high would allow more focussed cost-effective scab management. Graphical Abstract


2021 ◽  
Author(s):  
Jalal Aliabadi ◽  
Ehsan Rakhshandehroo ◽  
Azadeh Yektaseresht

Abstract Background The gastrointestinal helminth, Teladorsagia circumcincta, is one of the major health risk and production-limiting disease in small ruminant populations, particularly in temperate regions. With the increasing importance of the disease management and recruited anthelminic resistant types, accurate approaches are needed for diagnosis of the infection in the host. Because of uncertain results using faecal examinations, the ELISA method was indicated for detection of the nematode antigenic materials. Despite some promising results, problems were described in terms of the test specificity and the cross-reactions. The aim of this study was therefore to evaluate worm somatic and excretory/secretory (ES) products using western blot analysis and an indirect ELISA for detecting T. circumcincta infection in sheep. Results Based on the immuno-reactivity analysis, immunogenic fractions with molecular weights of approximately 60, 75 and 100 kDa were detected in somatic content and two antigens of about 63 and 75 kDa in ES material. Accordingly, a specific product at 75 kDa had the strongest reaction and appeared as the most common antigenic protein. In ELISA, all sera from the infected sheep revealed the OD rates above the calculated cut-off value with about two-fold greater in average. Negative control samples were also specifically recognized with the mean OD rate of about 1/3 of the estimated cut-off value. The cross-reaction test, using rabbit hyperimmune sera, did not show reactivity with ES antigens of other prevalent nematodes include Haemonchus contortus, Protostrongylus rufescens and Marshallagia marshalli. In contrast, a strong positive reaction was found with the somatic antigens of M. marshalli. Conclusions The results obtained here indicates the indirect ELISA method using the ES content enables distinguishing the T. circumcincta infected sheep with high specificity. Those antigenic ES peptides with 63 and particularly 75 kDa molecular weights should be more investigated due to the potential for serological diagnostic methods and immunoprotective targets in the host.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Diky Ramdani ◽  
Dwi Cipto Budinuryanto ◽  
Juju Julaeha

ABSTRACT. Turmeric extract (Curcuma longa L.) has the potential to be a natural anthelmintic and alternative to commercial deworming drugs for naturally-infected local sheep. A completely randomized design was used to compare the effect of 5 different oral treatments of turmeric extract tablets at 0 (TET-0), 200 mg (TET-200), 400 mg (TET-400), 800 mg (TET-800), and commercial Oxfendazole 225mg (Oxfen-225) on reducing endoparasites (fecal egg counts, FEC) of naturally-infected local yearling ewes at day-0 (before treatments), day-7, day-14, and day-21 (after treatments) using 4 replicates (n = 4). At day-0, all experimental ewes were naturally infected by FEC Strongyles nematode ranging from 85.0 ± 32.8 to 638 ± 230 eggs/g. Meanwhile, FEC Fasciola spp. and Paramphistomum spp. (Trematode), Monieza spp. (Cestode), dan Eimeria spp. (coccidia) were found a little in a small number of ewes. Therefore, further analysis focused on FEC Strongyles. On day-7, only Oxfen-225 and TET-800 treatments reduced (P0.05) FEC Strongyles by 100% and 64%, respectively. There was no difference (P0.05) in the increased or reduced percentages of FEC Strongyles infections among group treatments during day-14 and day-21 although only Oxfen-225 treatment showed a constant decrease. It seems that orally administering turmeric extract tablet at 800 mg have the potential to reduce FEC Strongyles in sheep by 64% although its anthelmintic potential is still weaker than commercial oxfendazole 225 mg.  (Pengaruh Ekstrak Kunyit (Curcuma longa L.) sebagai Antelmintik terhadap Penurunan Endoparasit pada Domba yang Terinfeksi secara Alami) ABSTRAK. Ekstrak kunyit (Curcuma longa L.) mempunyai potensi sebagai antelmintik alami pengganti obat cacing komersial pada domba lokal yang terinfeksi cacing. Rancangan acak lengkap digunakan untuk membandingkan pengaruh 5 perbedaan perlakuan ekstrak kunyit dalam bentuk tablet dengan dosis: 0 (TET-0), 200 mg (TET-200), 400 mg (TET-400), 800 mg (TET-800), dan Oxfendazole komersil 225mg (Oxfen-225) terhadap penurunan jumlah telur cacing pada feses (Fecal egg counts, FEC) domba betina muda yang terinfeksi cacing secara alami pada hari ke-0 (sebelum perlakuan), hari ke-7, hari ke-14, dan hari ke-21 setelah perlakuan menggunakan 4 ulangan (n = 4). Pada hari ke-0, semua domba eksperimen terinfeksi secara alami oleh nematoda Strongyles sebanyak 85.0 ± 32.8 sampai 638 ± 230 telur/gram feses. Sedangkan FEC Fasciola spp. dan Paramphistomum spp. (Trematoda), Monieza spp. (Cestoda), dan Eimeria spp. (Koksidia) hanya ada di beberapa domba saja dalam jumlah yang sedikit. Sehingga Analisa selanjutnya difokuskan kepada FEC Strongyles. Pada hari ke-7, hanya perlakuan Oxfen-225 dan Cur-800 yang dapat mengurangi (P0.05) persentase FEC Strongyles sebanyak 100% dan 64%, secara berurutan. Tidak ada perbedaan (P0.05) pada peningkatan atau penurunan persentase FEC Strongyles pada semua perlakuan selama hari ke-14 dan hari ke-21 walaupun perlakuan Oxfen-225 memperlihatkan pengurangan persentase FEC Strongyles secara konstan. Ekstrak kunyit dalam bentuk tablet pada dosis 800 mg mempunyai potensi menurunkan infeksi FEC Strongyles sekitar 64% pada minggu pertama, tetapi kemampuan antiparasitiknya masih di bawah oxfendazole 225 mg.


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