Intralobular distribution of preneoplastic foci in rat liver after a single dose of N-methyl-N-nitrosourea (MNU) following partial hepatectomy

This article is a review of the rat liver foci bioassay which is an initiation/promotion assay that utilizes preneoplastic foci of hepatocytes possessing altered enzymatic activity and altered cellular constituents as indicators of carcinogenesis. Rat liver tumor promoters that have been employed in the assay include: (1) a low level of 2-acetylaminofluorene in the diet, (2) phenobarbital in the diet and drinking water and (3) a choline deficient diet. Camma-glutamyl transpeptidase-positive, and adenosine triphosphatase, and glucose-6-phosphatase-defi-cient foci are used to indicate the initiation of cancer and are reviewed with respect to their relationship to cancer. Partial hepatectomy at the time of initiation increases the sensitivity of the assay to dialkylnitrosamines and chemicals which are otherwise negative in rat liver. The protocols being developed by the Health Effects Research Laboratory in Cincinnati include: (1) partial hepatectomy either 18 hours prior to or 14 days after administering the chemical under test, (2) phenobarbital promotion and (3) using gamma-glutamyl transpeptidase-positive foci as the indicator of carcinogenesis. The article also reviews the rat liver foci bioassay as a component of the Carcinogenesis Testing Matrix for tier II where it will be employed to confirm the carcinogenic hazard associated with environmental samples containing complex mixtures.

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Faculty Opinions recommendation of Large induction of type III deiodinase expression after partial hepatectomy in the regenerating mouse and rat liver.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1127038.584168 ◽

2008 ◽

Author(s):

Antonio Bianco

Keyword(s):

Partial Hepatectomy ◽

Rat Liver ◽

Type Iii

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AUTOSYNCHRONIZATION OF RAT LIVER CELLS WITH ENDOGENOUS CORTICOSTERONE AFTER PARTIAL HEPATECTOMY

Cell Proliferation ◽

10.1111/j.1365-2184.1975.tb01201.x ◽

1975 ◽

Vol 8 (1) ◽

pp. 1-9

Author(s):

L. Desser-Wiest

Keyword(s):

Partial Hepatectomy ◽

Rat Liver ◽

Liver Cells ◽

Rat Liver Cells

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DEOXYCYTIDYLATE DEAMINASE LEVELS IN REGENERATING RAT LIVER

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o61-105 ◽

1961 ◽

Vol 39 (6) ◽

pp. 1043-1054 ◽

Cited By ~ 24

Author(s):

D. K. Myers ◽

C. Anne Hemphill ◽

Constance M. Townsend

Keyword(s):

Dna Synthesis ◽

Liver Regeneration ◽

Partial Hepatectomy ◽

Rat Liver ◽

Deoxyribonucleic Acid ◽

Regenerating Liver ◽

Regenerating Rat Liver ◽

High Level ◽

Early Regeneration

Deoxycytidylate deaminase activity and net synthesis of deoxyribonucleic acid (DNA) in vivo were found to increase at approximately the same time during the early stages of liver regeneration. However, deaminase activity in the regenerating liver remained at a high level for 1 day after DNA synthesis had slowed down again during the later stages of regeneration. The increase in deaminase activity was restricted as a result of exposure to 600 r X radiation during early regeneration, but this effect only became evident 11–16 hours after the irradiation. Irradiation on the second day after partial hepatectomy, when deaminase levels in control regenerating livers were relatively constant, failed to affect the deaminase activity immediately but did produce a 40–50% decrease in activity 11–16 hours later. Other antimitotic agents, e.g., colchicine, had little effect on deaminase activity.

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