Unusual Features of the SARS-CoV-2 Genome Suggesting Sophisticated Laboratory Modification as a Biological Robot

The COVID-19 pandemic caused by the novel coronavirus SARS-CoV-2 has led to deaths worldwide and decimation of the global economy. Despite its tremendous impact, the origin of SARS-CoV-2 has remained mysterious and controversial. The natural origin theory, although widely accepted, lacks substantial support. The alternative theory that the virus may have come from a research laboratory is, however, censored on peer-reviewed scientific journals. Nonetheless, SARS-CoV-2 shows biological characteristics that are inconsistent with a naturally occurring, zoonotic virus. In this report, the authors describe the genomic, structural, medical, and literature evidence, which, when considered together, strongly contradicts the natural origin theory. The evidence shows that SARS-CoV2 should be a laboratory product created by using bat coronaviruses ZC45 and/or ZXC21 as a template and/or backbone. Building upon the evidence, the authors further postulate a synthetic route for SARS-CoV-2, demonstrating that the lab-creation of this coronavirus is convenient and can be accomplished in approximately six months.

Survey Analysis of Enhanced Oil Recovery (EOR) Laboratory towards Featured Research Laboratory

The EOR Research Laboratory is a laboratory that was independently pioneered by the Department of Petroleum Engineering UPN "Veteran" Yogyakarta. The EOR Research Laboratory needs to be improved especially for the existing spatial layout to support the ongoing and future research. This Institutional Research will cover the planning process of spatial layout design, spatial layout realization, internal and external EOR Research Laboratory socialization, and at the end of the study, effective and efficient governance will be applied to adapt to the current pandemic conditions. The method used in this research is quantitative in the form of socialization, questionnaires, and survey analysis to find out how EOR Laboratory is well known among students. This research is expected to be able to introduce the EOR Research Laboratory in a wider range and carrying out sustainable research in the future so that it will support the planning of the laboratory to be the Leading EOR Research Laboratory at the Department of Petroleum Engineering, UPN "Veteran" Yogyakarta.

Physiological and biochemical aspects of adaptation-compensatory processes of hydrobionts under the effect of technological factors

Purpose. To study the functional status in the body of the common carp Cyprinus carpio (Linnaeus, 1758) after the addition of biologically active substances in feed composition as an adaptogene and corrector of metabolic processes including the antioxidant system, catalase activity in carp body. Methodology. The experimental part of the study was performed at the laboratory of the Scientific and Research Laboratory “Aquaculture Perspectives”, Scientific and Research Laboratory of “S. Pentelyuk Physiological and Biochemical Studies”, Scientific and Research Laboratory of Ecological and Chemical Analysis and Water Monitoring of the State Education Institution “Kherson State Agrarian and Economical University” and at the laboratory of the “Kherson Production and Experimental Hatchery of Ordinary Fish Breeding”. The duration of the experimental part was 30 days. During this period, visual observation of the fish was carried out, biological material was collected for the study in accordance with the current methods in fish farming. Cytological studies of blood were carried out on smears using Micromed MDC-500-5MP eyepiece camera. ThermoMix equipment was used to transport biological material to the laboratory. The hydrochemical parameters in tanks of the recirculating aquaculture system (RAS) were investigated by express methods (tests) with the corresponding recording of results in the work journal. The reaction and ethology of the common carp Cyprinus carpio to the light was carried out using digital equipment and a LDT test. Findings. The obtained results demonstrated the positive effect of the feed factor as an adaptogene and growth stimulator on the redistribution of metabolic energy in the carp body, which in turn had a stimulating effect on the development rate and improved the general functional status of the fish body. The paper presents the results of morpho-functional, biochemical parameters of carp blood in ontogenesis when grown in recirculating aquaculture systems against the background of the application of elements to improve technological aspects while improving the adaptive capabilities of the fish body. It was found that the feed factor, when introduced into the general economic ration, promoted an increase in the average carp body weight in the experimental group, improved the parameters of homeostatic equilibrium in comparison with fish in the control group. Positive results of a scientific and experimental study on the use of the additive as an adaptogene were obtained; it was studied with the aim of activating protective mechanisms, metabolic processes of aquatic organisms under constant or systematic exposure to stress factors of a technological nature. The study showed that the body weight, yield, total number of erythrocytes, hemoglobin content, total protein, and the level of catalase activity in the experimental groups of fish exceeded these parameters in the control group. The results obtained allowed noting the activation of the antioxidant system, the restructuring of physiological and biochemical processes, mobilization systems in the carp body in the experimental group. Originality. We found a correcting effect of the feed factor on metabolic processes, the adaptive capacity of the fish organism, as well as an increase in the resistance of the body as a whole. After implementing the technological scheme of cultivation of a feed factor of natural origin as an adaptogene against the background of the influence of technological stress factors, we found a correlation between the morpho-functional, biochemical parameters of carp blood and stress markers. Addition of biologically active substances of natural origin with a pronounced adaptive and correcting physiological and biochemical parameters into the general diet of carp improved the adaptive capacity. The antioxidant system was activated against the background of the action of technological factors. Practical value. The results of scientific and experimental studies of the functional status of carp body under the influence of technological factors were presented. Parameters of the development of carp in ontogenesis when growing in recirculating aquaculture systems against the background of the introduction of multilevel elements to improve technological aspects were studied. The results demonstrated the positive effect of biologically active substances invlufrf in the feed mixture, in particular, activation of the growth rate, increase in body weight, and yield. Key words: functional state of the carp body, adaptive and compensatory parameters, development rate, recirculation systems, technological factors.

Trends in Chemical Wood Surface Improvements and Modifications: A Review of the Last Five Years

Increasing the use of wood in buildings is regarded by many as a key solution to tackle climate change. For this reason, a lot of research is carried out to develop new and innovative wood surface improvements and make wood more appealing through features such as increased durability, fire-retardancy, superhydrophobicity, and self-healing. However, in order to have a positive impact on the society, these surface improvements must be applied in real buildings. In this review, the last five years of research in the domain of wood surface improvements and modifications is first presented by sorting the latest innovations into different trends. Afterward, these trends are correlated to specifications representing different normative, ecologic and economic factors which must be considered when expecting to introduce a wood treatment to the market. With this review, the authors hope to help researchers to take into consideration the different factors influencing whether new innovations can leave the research laboratory or not, and thereby facilitate the introduction of new wood surface treatments in the society.

Culturomics revealed the bacterial constituents of the microbiota of a 10-year-old laboratory culture of planarian species S. mediterranea

The planarian species Schmidtea mediterranea is a flatworm living in freshwater that is used in the research laboratory as a model to study developmental and regeneration mechanisms, as well as antibacterial mechanisms. However, the cultivable microbial repertoire of the microbes comprising its microbiota remains unknown. Here, we characterized the bacterial constituents of a 10-year-old laboratory culture of planarian species S. mediterranea via culturomics analysis. We isolated 40 cultivable bacterial species, including 1 unidentifiable species. The predominant phylum is Proteobacteria, and the most common genus is Pseudomonas. We discovered that parts of the bacterial flora of the planarian S. mediterranea can be classified as fish pathogens and opportunistic human pathogens.

Artificial intelligence in aircraft design and the role of professor V.G. Maslov’s scientific school in the process of its development

The article was prepared for the 95th anniversary of the birth of Valentin Grigorievich Maslov, Doctor of Science (Engineering), Professor, founder of the school for optimal design of aircraft engine parameters, head of the engine CAD system department (Department 6) of the applied research laboratory (ONIL-2) of Kuibyshev Aviation Institute named after academician S.P. Korolyov. The stages of creating the theoretical foundation for optimal design of aircraft are discussed. The role of Professor V.G. Maslov in the formalization of knowledge in aircraft design in a context of uncertainty and the importance of multi-criteria assessment of design solutions are described. The work of Department 6 ONIL-2 (KuAI-SSAU), led by Valentin Grigorievich, provided Samara scientific school with a leading position in the field of optimal design of engines for aircraft of varying function. These works contributed to the formation of a modern understanding of the intellectualization of systems and the successful start of work in the field of their creation. The development of Professor V.G. Maslovs ideas in the works of his students is shown.

Power System Simulation Laboratory as a Modern Educational Tool

To ensure the success of the personnel of power systems are required not only deep theoretical knowledge’s but also practical skills, the acquisition of which in modern universities is associated with considerable difficulties. The paper provides an overview of opportunities to overcome part of these difficulties by creating automation and protective relaying testing educational and research laboratory. To generate testing signals power system emergency processes records and simulation software are used. For education purposes microprocessorbased, suitable for digital input testing signals terminal is created