Developmental Expression of Estrogen Receptor mRNA in the Rat Cerebral Cortex: A Nonisotopic in situ Hybridization Histochemistry Study

1992 ◽  
Vol 2 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Rajesh C. Miranda ◽  
C. Dominique Toran-Allerand
Keyword(s):  
Estrogen Receptor ◽  
Cerebral Cortex ◽  
In Situ Hybridization ◽  
Rat Cerebral Cortex ◽  
Developmental Expression ◽  
Hybridization Histochemistry ◽  
Receptor Mrna ◽  
In Situ Hybridization Histochemistry ◽  
Estrogen Receptor Mrna

scholarly journals Gastric Parietal Cells: Potent Endocrine Role in Secreting Estrogen as a Possible Regulator of Gastro-Hepatic Axis

Endocrinology ◽  
2002 ◽  
Vol 143 (8) ◽  
pp. 3162-3170 ◽  
Author(s):  
Takashi Ueyama ◽  
Nobuyuki Shirasawa ◽  
Mitsuteru Numazawa ◽  
Keiko Yamada ◽  
Momoko Shelangouski ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
In Situ Hybridization ◽  
Gastric Mucosa ◽  
Estrogen Receptor Α ◽  
Parietal Cells ◽  
Rt Pcr ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry ◽  
Gastric Parietal Cells

Abstract Estrogen, if it is produced in the gastrointestinal tract, may overflow into the systemic circulation in the case of increased portal-systemic shunting. This idea is in accord with a significant step-up in serum estradiol (E2) concentration in the portal vein of rats, compared with that in the artery. Gene expression of aromatase, estrogen synthetase, was demonstrated by RT-PCR in the gastric mucosa of male and female adult rats, equivalent to that in the ovary. Aromatase activity and production of E2 in the gastric mucosa were demonstrated by 3H2O assay and gas chromatography-mass spectrometry, and they were inhibited by aromatase inhibitor, 4-hydroxyandrostenedione. Conversion of 14C-androstenedione to 14C-E2 through 14C-testosterone in cultured gastric mucosa was also demonstrated. Parietal cells exhibited strong signals for aromatase mRNA and immunoreactive protein by in situ hybridization histochemistry and immunohistochemistry. Estrogen receptor α mRNA and immunoreactive protein were demonstrated in hepatocytes by RT-PCR, in situ hybridization histochemistry, and immunohistochemistry. Total gastrectomy reduced portal venous E2 concentration, without changing systemic E2 concentration, together with down-regulation of estrogen receptor α mRNA level in the liver. These findings indicate that gastric parietal cells play a potent endocrine role in secreting estrogen that may function as a regulator of the gastro-hepatic axis.

Localization of dopamine D2 receptor mRNA with non-radioactive in situ hybridization histochemistry

1992 ◽  
Vol 142 (2) ◽  
pp. 223-227 ◽  
Author(s):  
Nieske Brouwer ◽  
Henk Van Dijken ◽  
Marcel H.J. Ruiters ◽  
Jan-Douwe Van Willigen ◽  
Gert J. Ter Horst
Keyword(s):  
In Situ Hybridization ◽  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Hybridization Histochemistry ◽  
Receptor Mrna ◽  
In Situ Hybridization Histochemistry ◽  
Dopamine D2
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