Effect of thyroid hormone deficiency on developmental expression of Goα gene in the brain of neonatal rats by competitive RT-PCR and in situ hybridization histochemistry

2000 ◽  
Vol 864 (2) ◽  
pp. 195-204 ◽  
Author(s):  
Dong-Sheng Cai ◽  
Qing Su ◽  
Yuan Chen ◽  
Min Luo
Keyword(s):  
In Situ Hybridization ◽  
Thyroid Hormone ◽  
Developmental Expression ◽  
Hormone Deficiency ◽  
Neonatal Rats ◽  
Rt Pcr ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry ◽  
The Brain

scholarly journals Gastric Parietal Cells: Potent Endocrine Role in Secreting Estrogen as a Possible Regulator of Gastro-Hepatic Axis

Endocrinology ◽  
2002 ◽  
Vol 143 (8) ◽  
pp. 3162-3170 ◽  
Author(s):  
Takashi Ueyama ◽  
Nobuyuki Shirasawa ◽  
Mitsuteru Numazawa ◽  
Keiko Yamada ◽  
Momoko Shelangouski ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
In Situ Hybridization ◽  
Gastric Mucosa ◽  
Estrogen Receptor Α ◽  
Parietal Cells ◽  
Rt Pcr ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry ◽  
Gastric Parietal Cells

Abstract Estrogen, if it is produced in the gastrointestinal tract, may overflow into the systemic circulation in the case of increased portal-systemic shunting. This idea is in accord with a significant step-up in serum estradiol (E2) concentration in the portal vein of rats, compared with that in the artery. Gene expression of aromatase, estrogen synthetase, was demonstrated by RT-PCR in the gastric mucosa of male and female adult rats, equivalent to that in the ovary. Aromatase activity and production of E2 in the gastric mucosa were demonstrated by 3H2O assay and gas chromatography-mass spectrometry, and they were inhibited by aromatase inhibitor, 4-hydroxyandrostenedione. Conversion of 14C-androstenedione to 14C-E2 through 14C-testosterone in cultured gastric mucosa was also demonstrated. Parietal cells exhibited strong signals for aromatase mRNA and immunoreactive protein by in situ hybridization histochemistry and immunohistochemistry. Estrogen receptor α mRNA and immunoreactive protein were demonstrated in hepatocytes by RT-PCR, in situ hybridization histochemistry, and immunohistochemistry. Total gastrectomy reduced portal venous E2 concentration, without changing systemic E2 concentration, together with down-regulation of estrogen receptor α mRNA level in the liver. These findings indicate that gastric parietal cells play a potent endocrine role in secreting estrogen that may function as a regulator of the gastro-hepatic axis.

scholarly journals In situ hybridization histochemistry localization of interleukin-3 mRNA in mouse brain

Blood ◽  
1989 ◽  
Vol 73 (1) ◽  
pp. 137-140
Author(s):  
WL Farrar ◽  
M Vinocour ◽  
JM Hill
Keyword(s):  
In Situ Hybridization ◽  
Mouse Brain ◽  
Neuronal Cell Body ◽  
Northern Analysis ◽  
Molecular Evidence ◽  
Hybridization Histochemistry ◽  
Interleukin 3 ◽  
In Situ Hybridization Histochemistry ◽  
The Brain

The hematopoietic growth factor interleukin-3 (IL-3) promotes the proliferation and maturation of pluripotent myeloid progenitor cells. In the immune system, IL-3 is synthesized by mitogen or antigen- stimulated T lymphocytes. We demonstrate the expression of IL-3 mRNA in mouse brain by in situ hybridization histochemistry and Northern blot analysis. The IL-3 mRNA is localized in discrete areas of the brain and can be found in neuronal cell body and astrocytes. Northern analysis of cerebellar RNA, compared with mRNA extracted from WEHI-3 cells, showed a single hybridization band, approximately 1.2 kb, suggesting similar processing between brain and myeloid cells. The molecular evidence and previous observations of IL-3-like biologic activity found in the brain suggest a potential role for IL-3 in the neurobiology of the CNS.

scholarly journals In situ hybridization histochemistry localization of interleukin-3 mRNA in mouse brain

Blood ◽  
1989 ◽  
Vol 73 (1) ◽  
pp. 137-140 ◽  
Author(s):  
WL Farrar ◽  
M Vinocour ◽  
JM Hill
Keyword(s):  
In Situ Hybridization ◽  
Mouse Brain ◽  
Neuronal Cell Body ◽  
Northern Analysis ◽  
Molecular Evidence ◽  
Hybridization Histochemistry ◽  
Interleukin 3 ◽  
In Situ Hybridization Histochemistry ◽  
The Brain

Abstract The hematopoietic growth factor interleukin-3 (IL-3) promotes the proliferation and maturation of pluripotent myeloid progenitor cells. In the immune system, IL-3 is synthesized by mitogen or antigen- stimulated T lymphocytes. We demonstrate the expression of IL-3 mRNA in mouse brain by in situ hybridization histochemistry and Northern blot analysis. The IL-3 mRNA is localized in discrete areas of the brain and can be found in neuronal cell body and astrocytes. Northern analysis of cerebellar RNA, compared with mRNA extracted from WEHI-3 cells, showed a single hybridization band, approximately 1.2 kb, suggesting similar processing between brain and myeloid cells. The molecular evidence and previous observations of IL-3-like biologic activity found in the brain suggest a potential role for IL-3 in the neurobiology of the CNS.

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