A Collaborative Study: High-Pressure Liquid Chromatographic Determination of Carbadox and Pyrantel Tartrate in Animal Feeds

Abstract Four duplicate samples of cocoa-containing materials, a practice sample, and standards were submitted to the collaborators for theobromine and caffeine analysis by HPLC. In the method the samples are defatted with petroleum ether, and dried. The fat-free residue is then extracted with water and an aliquot is injected into the chromatograph. Compounds are quantitated by comparison with internal or external standards, either by peak height or peak area. Results for all the analyses showed that few of the values were more than 2 standard deviations from the mean. The method has been adopted as official first action.

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High Pressure Liquid Chromatographic Determination of Oxazepam Dosage Forms: Collaborative Study

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/66.4.864 ◽

1983 ◽

Vol 66 (4) ◽

pp. 864-866

Author(s):

Eileen S Bargo ◽

◽

E Aranda ◽

C Bonnin ◽

S Hauser ◽

...

Keyword(s):

High Pressure ◽

Collaborative Study ◽

Chromatographic Determination ◽

Reverse Phase ◽

High Pressure Liquid Chromatographic ◽

Coefficients Of Variation ◽

Liquid Chromatographic Method ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

Abstract A reverse phase high pressure liquid chromatographic method for the determination of oxazepam in tablets and capsules was collaboratively studied by 9 laboratories. Collaborators were supplied with 6 samples that included synthetic and commercial formulations. Tablet and capsule composites are diluted with methanol and filtered. Oxazepam is determined at 254 nm by using a C18 column. Mean recoveries of oxazepam from synthetic tablet and capsule formulations were 97.2 and 99.0%, respectively. Mean coefficients of variation for tablets and capsules ranged from 1.85 to 2.86%. The method has been adopted official first action.

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High Pressure Liquid Chromatographic Determination of Physostigmine Salicylate and Physostigmine Sulfate in Liquids and Ointments: Collaborative Study

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/65.1.132 ◽

1982 ◽

Vol 65 (1) ◽

pp. 132-137

Author(s):

Norlin W Tymes ◽

◽

G Briguglio ◽

C Corcoran ◽

R Everett ◽

...

Keyword(s):

High Pressure ◽

Collaborative Study ◽

Internal Standard ◽

Pharmaceutical Formulations ◽

Chromatographic Determination ◽

Reverse Phase ◽

High Pressure Liquid Chromatographic ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

Abstract Results of 11 laboratories are presented for the collaborative study of a proposed method for the quantitative reverse phase high pressure liquid chromatographic (HPLC) determination of physostigmine salicylate and physostigmine sulfate in pharmaceutical formulations. The samples consisted of commercial solution, injection, and ointment preparations, each containing one of the physostigmine salts. The physostigmine salt is extracted from ointments with acetonitrile after the ointment is dissolved in hexane. Liquid preparations are diluted directly. Physostigmine is determined at 254 nm on a C18 column by comparison with a physostigmine standard. Flurazepam hydrochloride is the internal standard. The method has been adopted official first action for the solution dosage form.

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High Pressure Liquid Chromatographic Determination of Carbadox and Pyrantel Tartrate in Swine Feed and Supplements

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/66.3.602 ◽

1983 ◽

Vol 66 (3) ◽

pp. 602-605

Author(s):

Dwight M Lowie ◽

Robert T Teague ◽

Floyd E Quick ◽

Clarence L Foster

Keyword(s):

High Pressure ◽

Chromatographic Determination ◽

High Pressure Liquid Chromatographic ◽

Coefficients Of Variation ◽

Wide Range ◽

Liquid Chromatographic Determination ◽

Reliable Procedure ◽

Liquid Chromatographic ◽

Pyrantel Tartrate

Abstract A rapid yet reliable procedure for the simultaneous extraction and assay of carbadox and pyrantel tartrate is described. The feed is extracted with water-acetonitrile-methanol and cleaned up on a short alumina column. The eluant is separated by high pressure liquid chromatography and the compounds are detected at different wavelengths. The drugs of interest are well resolved in all feeds studied. The procedure has also been applied to a wide range of feeds which contained either one of the drugs or both in combination. No significant interferences were observed. Spiked sample recoveries were 97% for carbadox and 101% for pyrantel tartrate. Ruggedness test coefficients of variation were 2.0% for carbadox and 2.1% for pyrantel tartrate.

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High Pressure Liquid Chromatographic Determination of Xanthomegnin in Grains and Animal Feeds

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/66.3.587 ◽

1983 ◽

Vol 66 (3) ◽

pp. 587-591

Author(s):

Allen S Carman ◽

Shia S Kuan ◽

Octave J Francis ◽

George M Ware ◽

Jean A Gaul ◽

...

Keyword(s):

High Pressure ◽

Chromatographic Determination ◽

Hplc Method ◽

High Pressure Liquid Chromatographic ◽

Animal Feeds ◽

Elapsed Time ◽

Absorbance Detection ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

Abstract A high pressure liquid chromatographic (HPLC) method is described for the determination of xanthomegnin in grains and mixed animal feeds at levels ranging from 150 to 1200 ng/g. This is equivalent to actual amounts of xanthomegnin injected on the HPLC system at from 15 to 120 ng/injection. Xanthomegnin is extracted with chloroform and 0.1M phosphoric acid. An aliquot of the crude extract is purified by column chromatography using a commercially available silica gel cartridge. Xanthomegnin is then separated from the remaining interferences by HPLC with a reverse phase C-8 column, and subsequently determined by absorbance detection at 405 nm. Elapsed time for the method from initial extraction to final HPLC determination is approximately 1 h. Recoveries of xanthomegnin added to grains and animal feeds at levels from 150 to 1200 ng/g averaged 82% with a coefficient of variation of 10.2%.

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High Pressure Liquid Chromatographic Determination of Antihistamine-Adrenergic Combination Products: Collaborative Study

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/64.3.564 ◽

1981 ◽

Vol 64 (3) ◽

pp. 564-569 ◽

Cited By ~ 1

Author(s):

William J Bachman ◽

◽

M Y Alpert ◽

E Bargo ◽

R E Draper ◽

...

Keyword(s):

High Pressure ◽

Collaborative Study ◽

Chromatographic Determination ◽

Chlorpheniramine Maleate ◽

High Pressure Liquid Chromatographic ◽

Coefficients Of Variation ◽

Liquid Chromatographic Method ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

Abstract A reverse phase high pressure liquid chromatographic method in which ion-pairing is used for the determination of combinations of pseudoephedrine hydrochloride with triprolidine hydrochloride or chlorpheniramine maleate in syrups and tablets was collaboratively studied by 8 laboratories. Collaborators were supplied with 12 samples including synthetic and commercial syrup formulations and commercial tablet composites. Mean recoveries of pseudoephedrine hydrochloride and triprolidine hydrochloride from synthetic syrup formulations were 100.5 and 99.6%, respectively. Mean recoveries of pseudoephedrine hydrochloride and chlorpheniramine maleate from synthetic syrups were 98.8 and 100.5%, respectively. Mean coefficients of variation for syrups and tablets ranged from 1.68 to 3.07% for pseudoephedrine hydrochloride, from 2.92 to 3.85% for triprolidine hydrochloride, and from 1.34 to 2.15% for chlorpheniramine maleate. The method has been adopted official first action.

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High Pressure Liquid Chromatographic Determination of Monoand Disaccharides in Presweetened Cereals: Collaborative Study

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/65.2.256 ◽

1982 ◽

Vol 65 (2) ◽

pp. 256-264 ◽

Cited By ~ 2

Author(s):

Lucian C Zygmunt ◽

◽

E Anderson ◽

B Behrens ◽

R Bowers ◽

...

Keyword(s):

High Pressure ◽

Collaborative Study ◽

Chromatographic Determination ◽

Hplc Method ◽

High Pressure Liquid Chromatographic ◽

Aoac Method ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic ◽

Water Alcohol

Abstract A collaborative study was conducted using a modified AOAC method (sugars in chocolate) for the determination of fructose, glucose, sucrose, and maltose in presweetened cereals by high pressure liquid chromatography (HPLC). Eight samples consisting of 6 products were analyzed in duplicate by the HPLC method and the AOAC Lane-Eynon method. The AOAC method was modified to use water-alcohol (1 + 1) and Sep-Pak C18 cartridges for sample cleanup. The HPLC results indicate precision comparable to the Lane-Eynon method and the chocolate method. The modified HPLC method has been adopted official first action.

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High Pressure Liquid Chromatographic Determination of Sodium Saccharin, Sodium Benzoate, and Caffeine in Soda Beverages: Collaborative Study

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/62.5.1011 ◽

1979 ◽

Vol 62 (5) ◽

pp. 1011-1019

Author(s):

Betsy B Woodward ◽

Gordon P Heffelfinger ◽

Dennis I Ruggles

Keyword(s):

High Pressure ◽

Sodium Benzoate ◽

Collaborative Study ◽

Chromatographic Determination ◽

High Pressure Liquid Chromatographic ◽

Saccharin Sodium ◽

Sodium Saccharin ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

Abstract A collaborative study for the determination of sodium saccharin, sodium benzoate, and caffeine in 3 types of soda beverage, cola, grape, and lemon-lime, has been completed using reverse phase high pressure liquid chromatography with a μC18 column and acetic acid mobile phase. Recoveries for sodium saccharin were 98.6, 98.0, and 99.1%; for sodium benzoate, 100.6, 102.6, and 100.6%; and for caffeine, 100.8, 101.4, and 101.1%, respectively. The method has been adopted as official first action.

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