Gas-Liquid Chromatographic Isolation, Identification, and Quantitation of Some Barbiturates, Glutethimide, and Diphenylhydantoin in Whole Blood

1972 ◽  
Vol 18 (11) ◽  
pp. 1343-1349 ◽  
Author(s):  
Rodney G Cooper ◽  
Mavis S Greaves ◽  
Goronwy Owen
Keyword(s):  
Quantitative Determination ◽  
Whole Blood ◽  
Chromatographic Method ◽  
Accurate Method ◽  
Retention Data ◽  
Qualitative And Quantitative ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Chromatographic Isolation

Abstract An accurate method is described for the qualitative and quantitative determination of 11 barbiturates, glutethimide, and diphenylhydantoin in a single extract obtained from 1 ml of whole blood, which is extracted with ether at pH 2 and separately but simultaneously gas-liquid chromatographed on a selective (3% OV-17) and a nonselective (5% OV-1) column. Retention data are given. Results for barbiturate obtained by the Broughton [Biochem. J.63, 207 (1956)] spectrophotometric method and this gas-liquid chromatographic method are compared. Blood from more than 30 patients has been analyzed, and data are given to illustrate some of the findings.

scholarly journals New Liquid Chromatographic Method for Determination of Rabeprazole Sodium in Coated Tablets

2004 ◽  
Vol 87 (4) ◽  
pp. 842-846 ◽  
Author(s):  
Cássia V Garcia ◽  
Clésio S Paim ◽  
Martin Steppe
Keyword(s):  
Quantitative Determination ◽  
Proton Pump ◽  
Chromatographic Method ◽  
Array Detector ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Validation Parameters ◽  
Rabeprazole Sodium ◽  
Liquid Chromatographic

Abstract Rabeprazole sodium is a proton pump inhibitor that covalently binds and inactivates the gastric parietal cell proton pump (H+/K+ ATPase). Little has been published about the quantitative determination of this drug. The aim of this research was to develop a new liquid chromatographic method for quantitative determination of rabeprazole in coated tablets. The system consisted of a Hypersil Keystone Betabasic C8 column (250 × 4.6 mm, 5 μm particle size), an isocratic acetonitrile–water (35 + 65) mobile phase at a flow rate of 1.0 mL/min, and a diode array detector set at 282 nm. The following validation parameters were evaluated: linearity, precision, accuracy, specificity, detection and quantitation limits, and robustness. The method showed good linearity in the concentration range of 10–70 μg/mL. The quantitation limit was 2.43 μg/mL, and the detection limit was 0.80 μg/mL. The intra- and interday precision data showed that the method has good reproducibility (relative standard deviation = 1.03). Accuracy and robustness were also evaluated, and the results were satisfactory. The mean recovery was 101.61%. The analysis of a placebo mixture demonstrated the method is also specific.

Gas-Liquid Chromatographic Quantitative Determination of Vanillin and Ethyl Vanillin in Foods

1974 ◽  
Vol 57 (2) ◽  
pp. 329-331
Author(s):  
Janis E Schlack ◽  
Joseph J Dicecco
Keyword(s):  
Quantitative Determination ◽  
Chromatographic Method ◽  
Methylene Chloride ◽  
Ethyl Vanillin ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A gas-liquid chromatographic method for vanillin and ethyl vanillin in foods has been devised. Vanillin and ethyl vanillin are extracted into methylene chloride, and the extract is purified, concentrated, and then chromatographed on an EGSS-X column. Recovery of vanillin and ethyl vanillin averaged 99.54 and 98.05%, respectively.

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