scholarly journals New Liquid Chromatographic Method for Determination of Rabeprazole Sodium in Coated Tablets

2004 ◽  
Vol 87 (4) ◽  
pp. 842-846 ◽  
Author(s):  
Cássia V Garcia ◽  
Clésio S Paim ◽  
Martin Steppe
Keyword(s):  
Quantitative Determination ◽  
Proton Pump ◽  
Chromatographic Method ◽  
Array Detector ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Validation Parameters ◽  
Rabeprazole Sodium ◽  
Liquid Chromatographic

Abstract Rabeprazole sodium is a proton pump inhibitor that covalently binds and inactivates the gastric parietal cell proton pump (H+/K+ ATPase). Little has been published about the quantitative determination of this drug. The aim of this research was to develop a new liquid chromatographic method for quantitative determination of rabeprazole in coated tablets. The system consisted of a Hypersil Keystone Betabasic C8 column (250 × 4.6 mm, 5 μm particle size), an isocratic acetonitrile–water (35 + 65) mobile phase at a flow rate of 1.0 mL/min, and a diode array detector set at 282 nm. The following validation parameters were evaluated: linearity, precision, accuracy, specificity, detection and quantitation limits, and robustness. The method showed good linearity in the concentration range of 10–70 μg/mL. The quantitation limit was 2.43 μg/mL, and the detection limit was 0.80 μg/mL. The intra- and interday precision data showed that the method has good reproducibility (relative standard deviation = 1.03). Accuracy and robustness were also evaluated, and the results were satisfactory. The mean recovery was 101.61%. The analysis of a placebo mixture demonstrated the method is also specific.

scholarly journals New High-Performance Liquid Chromatographic Method for Determination of Clopidogrel in Coated Tablets

2008 ◽  
Vol 91 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Juliana Sippel ◽  
Letcia L Sfair ◽  
Elfrides E S Schapoval ◽  
Martin Steppe
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Array Detector ◽  
Aqueous Sample ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic

Abstract A new high-performance liquid chromatographic method was developed and validated for clopidogrel determination in pharmaceutical formulations. The system consisted of an ACE 5 octadecylsilane (C18; 150 4.6 mm id), 5.0 m particle size column; methanol0.1 triethylamine (75 + 25, v/v), pH 5.3, mobile phase at a flow rate of 1.2 mL/min; and a diode array detector set at 220 nm. Specificity, linearity, precision, accuracy, and robustness were the parameters evaluated. The retention time for clopidogrel was 6.8 min. To estimate specificity, an aqueous sample solution was subjected to degradation by ultraviolet light and by acid, alkaline, and oxidation media. The peaks of degradation products did not interfere with the compound signal, and there was no interference when a placebo solution was analyzed. Linearity over a concentration range of 10.0 to 90.0 g/mL was shown (correlation coefficient = 0.9998). Low values of relative standard deviation indicated the adequate intraday and interday precision. The average recovery was found as 99.16. In the robustness test, small modifications to the mobile phase composition did not affect the determination of clopidogrel. The proposed method proved to be simple, fast, and cost efficient for the intended use.

scholarly journals Development and Validation of a Reversed-Phase High-Performance Liquid Chromatographic Method for the Simultaneous Determination of Amiloride Hydrochloride, Atenolol, Hydrochlorothiazide, and Chlorthalidone in Their Combined Mixtures

2009 ◽  
Vol 92 (2) ◽  
pp. 404-409 ◽  
Author(s):  
Abdalla A Elshanawane ◽  
Samia M Mostafa ◽  
Mohamed S Elgawish
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Ternary Mixtures ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic ◽  
Amiloride Hydrochloride

Abstract A high-performance liquid chromatographic method was developed for the simultaneous determination of 2 ternary mixtures containing amiloride hydrochloride, atenolol, hydrochlorothiazide, and chlorthalidone used in hypertension therapy. The use of cyanopropyl column results in satisfactory separation of both mixtures. The mobile phase consisted of 10 mM KH2PO4 buffer (pH 4.5) and methanol in a ratio of (75 25 v/v), at a flow rate of 1 mL/min. UV detector was operated at 275 nm. Calibration graphs were linear in the concentration ranges of 210, 20200, 10100, and 550 g/mL for amiloride hydrochloride, atenolol, hydrochlorothiazide, and chlorthalidone, respectively. Intraday and interday precision values (relative standard deviation) were <1.13 for mixture I (amiloride hydrochloride, atenolol, chlorthalidone), and <0.93 for mixture II (amiloride hydrochloride, atenolol, hydrochlorothiazide). The method was successfully applied for the determination of the 2 combinations in laboratory-prepared mixtures and commercial pharmaceutical formulation with high accuracy and precision. Statistical comparison of the results with those of the published methods showed excellent agreement and indicates no significant difference between them.

A High-Performance Liquid Chromatographic Method for the Determination of Meropenem in Serum

2019 ◽  
Vol 58 (2) ◽  
pp. 144-150
Author(s):  
Demet Dincel ◽  
Olcay Sagirli ◽  
Gulacti Topcu
Keyword(s):  
Human Serum ◽  
High Performance ◽  
Chromatographic Method ◽  
Acetic Acid Solution ◽  
High Performance Liquid Chromatographic ◽  
Array Detector ◽  
Therapeutic Drug ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract In this study, a new, sensitive and selective high-performance liquid chromatographic method was developed for the determination of meropenem (MEM) in human serum. In the developed method, C18 column (3.9 × 150 mm, 5 μm) was selected as stationary phase at 30°C, and methanol: acetic acid solution mixture was used as mobile phase with gradient program. Chromatographic separation was carried out at a flow rate of 1 mL/min, and detection was performed at 300 nm with diode array detector. Doripenem was selected as an internal standard, and the analytes were extracted from serum using protein precipitation method with ortho-phosphoric acid: methanol. Detection wavelength was selected as 300 nm. The developed method was validated according to International Council for Harmonisation (ICH) guidelines. The calibration curve was linear over a concentration range of 4–240 μg/mL with correlation coefficient of 0.9985. The limit of detection and limit of quantification values were found as 0.057 and 0.192 μg/mL, respectively. The validated method was successfully applied for the determination of MEM in human serum samples collected from patient volunteers at different time intervals, and therapeutic drug monitoring of MEM has been investigated.

Gas-Liquid Chromatographic Isolation, Identification, and Quantitation of Some Barbiturates, Glutethimide, and Diphenylhydantoin in Whole Blood

1972 ◽  
Vol 18 (11) ◽  
pp. 1343-1349 ◽  
Author(s):  
Rodney G Cooper ◽  
Mavis S Greaves ◽  
Goronwy Owen
Keyword(s):  
Quantitative Determination ◽  
Whole Blood ◽  
Chromatographic Method ◽  
Accurate Method ◽  
Retention Data ◽  
Qualitative And Quantitative ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Chromatographic Isolation

Abstract An accurate method is described for the qualitative and quantitative determination of 11 barbiturates, glutethimide, and diphenylhydantoin in a single extract obtained from 1 ml of whole blood, which is extracted with ether at pH 2 and separately but simultaneously gas-liquid chromatographed on a selective (3% OV-17) and a nonselective (5% OV-1) column. Retention data are given. Results for barbiturate obtained by the Broughton [Biochem. J.63, 207 (1956)] spectrophotometric method and this gas-liquid chromatographic method are compared. Blood from more than 30 patients has been analyzed, and data are given to illustrate some of the findings.

Liquid Chromatographic Method for Determination of Glycerol in Wine and Grape Juice: Collaborative Study

1992 ◽  
Vol 75 (3) ◽  
pp. 379-383 ◽  
Author(s):  
Arthur Caputi ◽  
Eric Christensen ◽  
Nancy Biedenweg ◽  
Susan Miller
Keyword(s):  
Chromatographic Method ◽  
Collaborative Study ◽  
Grape Juice ◽  
External Standard Method ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
External Standard ◽  
Standard Deviations ◽  
Liquid Chromatographic

Abstract An Ion-exchange liquid chromatographic method for the determination of glycerol in wine, white grape juice, and pink grape juice was collaboratively studied by 8 laboratories. Eight wine types and 12 juice samples were provided to each collaborator. Using a strong cation column, blind duplicates and standards were analyzed by an external standard method. Separate statistical evaluations were run on wine, white grape juice, and pink grape juice data. The averages of the relative standard deviations for repeatability, excluding outlying results, were 1.25% for the wine samples, 7.32% for the white grape juice samples, and 8.63% for the pink grape juice samples. The averages of the relative standard deviations for reproducibility, excluding outlying results, were 2.79% for the wine samples, 16.97% for the white grape juice samples, and 19.10% for the pink grape juice samples. The method has been adopted first action by AOAC International.

Liquid Chromatographic Method for Multiresidue Determination of Benzimidazoles in Beef Liver and Muscle: Collaborative Study

1991 ◽  
Vol 74 (3) ◽  
pp. 487-493 ◽  
Author(s):  
Leon W Levan ◽  
Charlie J Barnes
Keyword(s):  
Chromatographic Method ◽  
Collaborative Study ◽  
Study Data ◽  
Beef Liver ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Repeatability And Reproducibility ◽  
Multiresidue Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method for determination of thiabendazole, 5-hydroxythiabendazole, oxfendazole, mebendazole (MBZ), and fenbendazole (FBZ) In cattle liver and muscle was collaboratively studied in 7 laboratories In 1986. For blind fortified samples containing 800 ppb FBZ, average recovery and relative standard deviations for repeatability and reproducibility (RSDr and RSDR) based on results from 6 of the participating laboratories were 83%, 12.7%, and 14.0%, respectively. Recoveries of FBZ from incurred liver samples were more variable. Recoveries of MBZ from livers fortified at the 100 ppb level were encouraging; however, the drug levels were too low in the incurred samples used for MBZ studies. Except for FBZ and MBZ In liver, the study data were not satisfactory. The method has been adopted official first action by AOAC for determination of 800-1600 ppb fenbendazole In liver. The analysis should be repeated using a smaller sample size when Initial analyses show levels greater than 1600 ppb FBZ.

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