Glyceraldehyde-phosphate dehydrogenase (total and isoenzyme activity) in the early diagnosis of myocardial infarction.

1977 ◽  
Vol 23 (2) ◽  
pp. 245-249 ◽  
Author(s):  
J Griffiths ◽  
S Shaw
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Early Diagnosis ◽  
Creatine Kinase Activity ◽  
Glyceraldehyde Phosphate Dehydrogenase ◽  
Major Disadvantage ◽  
Creatine Kinase Mb ◽  
Total Creatine Kinase Activity ◽  
In Vitro Stability

Abstract Enzyme "panels," in which creatine kinase and lactate dehydrogenase activities in serum are measured, are useful indicators of myocardial infarction. We examined a further enzyme, glyceraldehyde-phosphate dehydrogenase (EC 1.2.1.12), by comparison with creatine kinase (EC 2.7.3.2), in the early diagnosis of such infarctions. Results indicate that this total dehydrogenase appears in the serum before total creatine kinase activity; however, the lack of cardio-specificity relating to the dehydrogenase isoenzyme fraction 2 in comparison to the creatine kinase MB band is a major disadvantage, as is its relatively poor in vitro stability. We conclude that measurement of this dehydrogenase does not allow a substantially earlier diagnosis of myocardial infarction.

Use of creatine kinase MB isoenzyme for diagnosing myocardial infarction when total creatine kinase activity is high.

1988 ◽  
Vol 34 (11) ◽  
pp. 2208-2210 ◽  
Author(s):  
W G Thompson ◽  
R G Mahr ◽  
W S Yohannan ◽  
M R Pincus
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Urban Hospital ◽  
Population Total ◽  
Creatine Kinase Mb ◽  
Total Creatine Kinase Activity ◽  
Total Creatine ◽  
Indigent Population

Abstract The usefulness of measuring creatine kinase MB isoenzyme for diagnosing myocardial infarction when activities of total creatine kinase are very high is unclear. We conducted a retrospective study in an urban hospital that serves a largely indigent population. We concentrated on 146 patients whose creatine kinase activity was greater than 1000 U/L (upper limit of normal: 165 U/L for women and 225 U/L for men), with MB isoenzyme greater than 10 U/L and less than 5% of total creatine kinase. The positive predictive value of MB isoenzyme (isoimmune method) values greater than 10 U/L was between 11.6% and 56.8% when the value for total creatine kinase exceeded 1000 U/L. Using different values (MB greater than 4% of total creatine kinase) as positive for myocardial infarction would have resulted in far fewer false-positives, but 10 cases of myocardial infarction would have been missed. The most appropriate cutoff value for MB isoenzyme in this population (total creatine kinase greater than 1000 U/L) was found to be greater than 2% of total creatine kinase.

Myoglobin, creatine kinase MB isoforms and creatine kinase MB mass in early diagnosis of myocardial infarction in patients with acute chest pain

2002 ◽  
Vol 35 (8) ◽  
pp. 647-653 ◽  
Author(s):  
Karri Penttilä ◽  
Heli Koukkunen ◽  
Matti Halinen ◽  
Tapio Rantanen ◽  
Kalevi Pyörälä ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Chest Pain ◽  
Early Diagnosis ◽  
Acute Chest Pain ◽  
Creatine Kinase Mb

Development and clinical evaluation of a microcentrifugal analyzer method for determining creatine kinase MB isoenzyme.

1983 ◽  
Vol 29 (3) ◽  
pp. 533-538
Author(s):  
T H Massey ◽  
W C Butts
Keyword(s):  
Creatine Kinase ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Serum Samples ◽  
Inhibitory Antibody ◽  
Creatine Kinase Mb ◽  
Electrophoresis Method ◽  
Total Creatine Kinase Activity ◽  
Coronary Care ◽  
Total Creatine

Abstract We have adapted to a microcentrifugal analyzer an immunoinhibition assay for measuring the activity of creatine kinase MB by using an inhibitory antibody for the M monomer. The method actually measures half the MB activity, but results are not multiplied by two because atypical isoenzymes of creatine kinase, including BB, IgG-BB, and the isoenzyme derived from mitochondria, are also detected, if they are present. Results correlated well with an electrophoresis method for 36 serum samples. Myocardial infarction was assessed in 175 patients admitted to our coronary-care unit, with respect to sensitivity (100%) and specificity (98%) when a decision point of 100 U/L (30 degrees C) was chosen for total creatine kinase activity (dithiothreitol-activated) and 6 U/L (30 degrees C) for the isoenzyme (by immunoinhibition). Atypical isoenzymes are easily recognized and confirmed by electrophoresis when the MB activity (by immunoinhibition) exceeds 6 U/L and 20% of the total creatine kinase activity.

scholarly journals Comparison of serum myoglobin and creatine kinase MB isoenzyme in early diagnosis of acute myocardial infarction.

Heart ◽  
1981 ◽  
Vol 45 (4) ◽  
pp. 389-392 ◽  
Author(s):  
A P Freeman ◽  
K R Fatches ◽  
I W Carter ◽  
M J Cloonan ◽  
D E Wilcken
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Creatine Kinase ◽  
Early Diagnosis ◽  
Creatine Kinase Mb ◽  
Serum Myoglobin

Secretion of creatine kinase MB isoenzyme by an immature teratoma with predominant rhabdomyosarcomatous elements.

1988 ◽  
Vol 34 (12) ◽  
pp. 2600-2602 ◽  
Author(s):  
K Emancipator ◽  
A P Kudelka ◽  
G Bradford ◽  
K A Leonard ◽  
M H Zarrabi
Keyword(s):  
Creatine Kinase ◽  
Coronary Atherosclerosis ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Immature Teratoma ◽  
Malignant Teratoma ◽  
Creatine Kinase Mb ◽  
Total Creatine Kinase Activity ◽  
Total Creatine ◽  
Increased Activity

Abstract A 37-year-old man with metastatic immature (malignant) teratoma with prominent rhabdomyosarcomatous elements had markedly increased activity of creatine kinase (EC 2.7.3.2) MB in serum. There was no electrocardiographic evidence of infarction or ischemia, and autopsy revealed no myocardial infarction, significant coronary atherosclerosis, myocarditis, or invasion of the heart by tumor. A high proportion of the creatine kinase activity in a homogenate of the tumor was attributable to the MB isoenzyme. Persistent increases of creatine kinase-MB and an unusually high MB isoenzyme activity, out of proportion to total creatine kinase activity, may indicate a nonmyocardial origin of this isoenzyme.

Sign in / Sign up

Export Citation Format

Share Document