Continuous-flow enzymic method evaluated for measurement of serum triglycerides with use of an improved lipase reagent.

1978 ◽  
Vol 24 (11) ◽  
pp. 2018-2019 ◽  
Author(s):  
K Whitlow ◽  
N Gochman
Keyword(s):  
Continuous Flow ◽  
Flow System ◽  
Free Glycerol ◽  
Fluorometric Method ◽  
Serum Triglycerides ◽  
Microbial Lipase ◽  
Flow Method ◽  
Dual Channel ◽  
Continuous Flow Method ◽  
Total Triglyceride

Abstract We compared a modified enzymic continuous-flow method for serum triglycerides, in which a microbial lipase is used without protease, to an extraction-fluorometric method. Results (for total--blank) correlated well with those by the comparison procedure: enzymic = 1.002 fluorometric--14 mg/liter; r = 0.9968. Serum blanks were measured for each sample in both methods, and the concentration of free glycerol measured by the enzymic method was shown to be as much as 15 to 71% of the total triglyceride result in 7% of the samples analyzed. The dual-channel continuous-flow system provides automatic free-glycerol blank subtraction for the enzymic method.

Manual and continuous-flow colorimetry of triacylglycerols by a fully enzymic method.

1979 ◽  
Vol 25 (2) ◽  
pp. 273-278 ◽  
Author(s):  
R E Megraw ◽  
D E Dunn ◽  
H G Biggs
Keyword(s):  
Continuous Flow ◽  
Colorimetric Assay ◽  
Tetrazolium Salt ◽  
Good Precision ◽  
Free Glycerol ◽  
Serum Triglycerides ◽  
Precision And Accuracy ◽  
Glycerol 3 Phosphate Dehydrogenase ◽  
Microbial Esterase ◽  
Continuous Flow Method

Abstract We describe a fully enzymic method for manual and continuous-flow colorimetric assay of triacylglycerols (triglycerides) in serum. Triglycerides are enzymically hydrolyzed in 10 min by lipase and microbial esterase. The resulting free glycerol is measured enzymically by glycerol kinase and glycerol-3-phosphate dehydrogenase. The NADH so formed is oxidized by coupling with a tetrazolium salt/diaphorase system. The test follows Beer's law to 8 g/L, and the final color is stable for at least 1 h for serum, 15 min for aqueous triolein standards. The manual assay requires only 25 microliter of serum and few manipulations. A specific triolein standard was developed for calibrating the manual method. For the continuous-flow method, calibration is made with four concentrations of glycerol standard. The procedure is sensitive, has good precision and accuracy, and gives results that compare well with chemical and enzymic commercial kit methods.

A stopped-flow/continuous-flow method for kinetic determinations

1995 ◽  
Vol 309 (1-3) ◽  
pp. 277-282 ◽  
Author(s):  
Yun-Sheng Hsieh ◽  
S.R. Crouch
Keyword(s):  
Continuous Flow ◽  
Stopped Flow ◽  
Flow Method ◽  
Continuous Flow Method

scholarly journals Elucidation of the photoreactivity of nanocrystalline 2-hydroxychalcones using continuous flow method

2019 ◽  
Vol 75 (a1) ◽  
pp. a442-a442
Author(s):  
Sobiya George ◽  
Jeanette A. Krause ◽  
Anna D. Gudmundsdottir
Keyword(s):  
Continuous Flow ◽  
Flow Method ◽  
Continuous Flow Method

ZnO-Based Catalyst for Photodegradation of 2-Chlorophenol in Aqueous Solution Under Simulated Solar Light Using a Continuous Flow Method

JOM ◽  
2020 ◽  
Vol 73 (1) ◽  
pp. 404-410
Author(s):  
Ahed H. Zyoud ◽  
Hala Salah ◽  
Shaher H. Zyoud ◽  
Samer H. Zyoud ◽  
Muath H. Helal ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Continuous Flow ◽  
Solar Light ◽  
Flow Method ◽  
Simulated Solar Light ◽  
Continuous Flow Method

Controlled continuous flow method for transurethral resections

Urology ◽  
1983 ◽  
Vol 21 (2) ◽  
pp. 130-131 ◽  
Author(s):  
Jerrold Widran
Keyword(s):  
Continuous Flow ◽  
Flow Method ◽  
Continuous Flow Method

In Situ Generated Iron Oxide Nanocrystals as Efficient and Selective Catalysts for the Reduction of Nitroarenes using a Continuous Flow Method

2012 ◽  
Vol 124 (40) ◽  
pp. 10337-10340 ◽  
Author(s):  
David Cantillo ◽  
Mostafa Baghbanzadeh ◽  
C. Oliver Kappe
Keyword(s):  
Iron Oxide ◽  
Continuous Flow ◽  
Flow Method ◽  
Continuous Flow Method

Continuous-flow fluorometry of low galactose concentrations in blood or plasma.

1980 ◽  
Vol 26 (2) ◽  
pp. 282-285 ◽  
Author(s):  
J M Henderson ◽  
F W Fales
Keyword(s):  
Blood Flow ◽  
Hepatic Blood Flow ◽  
Continuous Flow ◽  
Normal Subjects ◽  
Galactose Oxidase ◽  
Fluorogenic Substrate ◽  
Flow Method ◽  
Peroxidase Reaction ◽  
Hydroxyphenylacetic Acid ◽  
Continuous Flow Method

Abstract Clearance of 0-100 mg/L concentrations of galactose from the blood depends on nutrient hepatic blood flow. We can measure such concentrations, which was not previously possible, by a continuous-flow method involving the use of galactose oxidase and peroxidase, the latter being coupled to a fluorogenic substrate, p-hydroxyphenylacetic acid. Interfering substances in the peroxidase reaction are removed by zinc/alkali precipitation. Sensitivity is maximized by using saturating concentrations of the enzymes and substrate. In prepared plasma test samples with galactose concentrations of 10, 40, 70, and 100 mg/L, the within-run CV's ranged from 2.1 to 8.6%, and day-to-day CV's from 2.2 to 17.2%, the largest CV's being for the 10 mg/L concentration. Normal subjects are shown to clear galactose more efficiently than subjects with moderate cirrhosis.

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