Sample preparation with ion-exchange resin before liquid-chromatographic determination of plasma catecholamines.

1983 ◽  
Vol 29 (7) ◽  
pp. 1426-1428 ◽  
Author(s):  
K N Frayn ◽  
P F Maycock
Keyword(s):  
Cation Exchange ◽  
High Performance ◽  
Exchange Resin ◽  
Plasma Catecholamines ◽  
Cation Exchange Resin ◽  
Chromatographic Determination ◽  
Ion Exchange Resin ◽  
Plasma Catecholamine ◽  
Plasma Catecholamine Concentrations ◽  
Liquid Chromatographic

Abstract Estimation of plasma catecholamine concentrations by "high-performance" liquid chromatography, although increasingly used, still presents difficulties. We describe a method for clean-up of plasma samples on cation-exchange resin before they are extracted with alumina. This procedure eliminates many of the interfering compounds often present in plasma extracts prepared with the alumina treatment alone. The method can be adapted to use commercially available disposable cation-exchange columns.

Preparation of urine samples for liquid-chromatographic determination of catecholamines: bonded-phase phenylboronic acid, cation-exchange resin, and alumina adsorbents compared.

1985 ◽  
Vol 31 (2) ◽  
pp. 298-302 ◽  
Author(s):  
A H Wu ◽  
T G Gornet
Keyword(s):  
Cation Exchange ◽  
Exchange Resin ◽  
Phenylboronic Acid ◽  
Cation Exchange Resin ◽  
Chromatographic Determination ◽  
Urinary Catecholamines ◽  
Weak Cation Exchange ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract We compared results for the liquid-chromatographic determination of free norepinephrine and epinephrine in urine after purifying the catechols by the following methods: (a) acid-washed alumina, (b) weak cation-exchange resin (WCX), (c) a combination of weak cation-exchange resin followed by alumina (WCX-alumina), and (d) commercially available phenylboronic acid adsorbent. We evaluated analytical specificity, sensitivity, recovery, and turnaround time. The WCX-alumina combination produced the most sensitive and specific chromatograms for urinary catecholamines; the other methods took less processing time. Neither WCX nor alumina alone was suitable for routine work because of chromatographic interferences in a significant proportion of urines. The phenylboronic acid method is adequately sensitive and specific for norepinephrine and epinephrine, and samples can be assayed faster. Thus it provides a compromise between the high analytical performance of the WCX-alumina method and the speed of the WCX and alumina methods.

Sign in / Sign up

Export Citation Format

Share Document