An anion-exchange chromatographic method for measuring bilirubin covalently bound to albumin.

1985 ◽  
Vol 31 (8) ◽  
pp. 1317-1321 ◽  
Author(s):  
D Seligson ◽  
H Seligson ◽  
T W Wu
Keyword(s):  
Liquid Chromatography ◽  
Anion Exchange ◽  
Chromatographic Method ◽  
Total Bilirubin ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
The Other ◽  
Covalently Linked ◽  
Original Amount ◽  
Covalently Bound

Abstract After alkalinizing the diazo products of serum bilirubins, we apply them to a column of anion-exchange resin. The azodipyrroles derived from unconjugated and sugar-conjugated bilirubins, as well as from one half of the tetrapyrrole covalently linked to albumin ("Bil-Alb"), are anionic and protein-free and adsorb to the resin. The other half of the azo product of Bil-Alb, with absorptivity similar to that of the protein-free azodipyrroles, remains attached to albumin (Clin Chem 28:629-637, 1982) and passes through the resin unadsorbed. The color of the eluate correlates directly with the original amount of Bil-Alb present. Estimates of authentic Bil-Alb by the method agree with those by a Jendrassik-Gróf total-bilirubin method (in mg/L: intercept = -0.8, slope = 1.005, r = 0.999) and by liquid chromatography (Clin Chem 29:800-805, 1983) (n = 52, intercept = 5.2, slope = 0.724, r = 0.965). The CV of the method for serum with Bil-Alb of 99.4 mg/L was 3.8% (n = 8).

scholarly journals Tryptic phosphopeptides from whole casein. I. Preparation and analysis by fast protein liquid chromatography

1989 ◽  
Vol 56 (4) ◽  
pp. 603-611 ◽  
Author(s):  
Marcel A. Juillerat ◽  
Robert Baechler ◽  
Raphael Berrocal ◽  
Serge Chanton ◽  
Jean-Claude Scherz ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Anion Exchange ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Gradient Elution ◽  
Fast Protein Liquid Chromatography ◽  
Parent Species ◽  
Net Charge ◽  
Phosphorylated Peptides ◽  
Salt Gradient

SummaryTryptic phosphopeptides were obtained from whole bovine casein by chromatography on the anion exchange resin QAE-Sephadex A 25. Salt gradient elution of the column allowed separation of non-phosphorylated peptides from phosphorylated species. The preparations obtained contained at least seven distinct phosphopeptides of which the following casein fragments were identified: αs1(43–58):2P, αs1(59–79): 5P, αs2(46–70): 4P, β(1–28): 4P, β(2–28): 4P, and β(33–48): 1P. Fast protein liquid chromatography (FPLC) on Mono Q HR 5/5 resin showed that the phosphopeptides were eluted in the same order as from the QAE-Sephadex resin. However, on the analytical column HR 5/5 the fragments αs1(59–79): 5P and β(2–28): 4P, having the same net charge under the conditions of chromatography, co-eluted, whereas they were at least partly separated on the preparative column HR 16/10. Following enzymic dephosphorylation, the peptides eluted at lower salt strength in the gradient. FPLC on Mono Q resin thus permitted dephosphorylation to be monitored and intermediates between the parent species and the fully dephosphorylated peptide to be identified.

scholarly journals Novel Application of Hybrid Anion Exchange Resin for Phosphate Desorption Kinetics in Soils: Minimizing Re-Adsorption of Desorbed Ions

Soil Systems ◽  
2020 ◽  
Vol 4 (2) ◽  
pp. 36
Author(s):  
Zhe Li ◽  
Suwei Xu ◽  
Ying Li ◽  
Yuji Arai
Keyword(s):  
Anion Exchange ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
The Other ◽  
Desorption Kinetics ◽  
Order Kinetic ◽  
Hybrid Resin ◽  
Phosphate Desorption ◽  
P Desorption ◽  
Resin Method

The process of phosphate desorption from soils is difficult to measure using stirred batch techniques because of the accumulation of desorbed ions in a bathing solution. To accurately measure the apparent rate coefficient of phosphate desorption from soils, it is necessary to remove the desorbed ions. In this study, a novel hybrid (i.e., iron oxide coated) anion exchange resin was used as a sink to study long-term (seven days) P desorption kinetics in intensively managed agricultural soils in the Midwestern U.S. (total phosphorus (TP): 196–419 mg/kg). The phosphate desorption kinetics in the hybrid anion exchange resin method were compared with those in the other conventional batch desorption method with pure anion exchange resins or without any sink. The extent of P desorption in the hybrid resin methods was >50% of total desorbed phosphate in the other methods. The initial kinetic rate estimated in the pseudo-second-order kinetic model was also highest (3.03–31.35 mg/(g·hr)) in the hybrid resin method when the same soil system was compared. This is because adsorbed P in the hybrid resins was nearly irreversible. The hybrid anion exchange resin might be a new and ideal sink in measuring the P desorption process in soils and sediments.

scholarly journals Liquid chromatography of mono-and di-substituted benzene derivatives with anion-exchange resin and ethanol

1971 ◽  
Vol 20 (4) ◽  
pp. 427-430 ◽  
Author(s):  
Wataru FUNASAKA ◽  
Teiichi ANDO ◽  
Kazumi FUJIMURA ◽  
Toshihiko HANAI
Keyword(s):  
Liquid Chromatography ◽  
Anion Exchange ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Benzene Derivatives

Liquid chromatography of phenolic compounds on a microbore anion exchange resin column

1984 ◽  
Vol 56 (8) ◽  
pp. 1525-1527 ◽  
Author(s):  
Krishnat P. Naikwadi ◽  
Souji. Rokushika ◽  
Hiroyuki. Hatano
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Anion Exchange ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Resin Column

Liquid-chromatographic measurement of urinary monosaccharides.

1980 ◽  
Vol 26 (9) ◽  
pp. 1348-1350 ◽  
Author(s):  
M D'Amboise ◽  
T Hanai ◽  
D Noël
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Anion Exchange ◽  
High Performance ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Reduction Reaction ◽  
Limits Of Detection ◽  
Chromatographic Measurement ◽  
Liquid Chromatographic

Abstract We describe "high-performance" liquid chromatography of urinary monosaccharides on a macroporous anion-exchange resin (Hitachi 3013N), with acetonitrile/water as eluent. The separated xylose, arabinose, D-fructose, mannose, galactose, and D-glucose were detected by means of a post-column reduction reaction of tetrazolium blue. Absolute limits of detection were about 10 ng of monosaccharide. Lactose can also be measured.

Glucosinolates and Derived Products in Cruciferous Vegetables: Total Glucosinolates by Retention on Anion Exchange Resin and Enzymatic Hydrolysis to Measure Released Glucose

1977 ◽  
Vol 60 (4) ◽  
pp. 946-949 ◽  
Author(s):  
Cecil H VanEtten ◽  
Melvin E Daxenbichler
Keyword(s):  
Liquid Chromatography ◽  
Enzymatic Hydrolysis ◽  
Anion Exchange ◽  
Coefficient Of Variation ◽  
Methylene Chloride ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Gas Liquid Chromatography ◽  
Other Substances ◽  
Hydrolysis Of

Abstract Details are given for determining total glucoginolates in Cruciferae plants by a procedure measuring released glucose. The glucosinolates are separated from about 90% of other material in the plant extract by adsorption on an anion exchange resin. Then, by a selective thioglucosidase hydrolysis of the glucosinolates retained on the exchange resin, the glucose and aglucons are separated from other substances retained by the resin. Glucose is released into an aqueous medium and is equivalent to the total glucosinolates. The aglucons formed by the hydrolysis are extracted into methylene chloride and determined by gas-liquid chromatography. Based on 29 determinations of the glucose from sinigrin, analyzed under different conditions, accuracy of the total glucosinolate determination was 94.8 ± 7.3%. The coefficient of variation, determined by duplicate analyses on extracts from 58 cabbage samples, was 4.6%.

Delta bilirubin in serum of pediatric patients: correlations with age and disease.

1984 ◽  
Vol 30 (9) ◽  
pp. 1561-1564 ◽  
Author(s):  
E M Brett ◽  
J M Hicks ◽  
D M Powers ◽  
R N Rand
Keyword(s):  
Liquid Chromatography ◽  
Biliary Atresia ◽  
Chromatographic Method ◽  
Pediatric Patients ◽  
Total Bilirubin ◽  
Infants And Children ◽  
Biliary Cirrhosis ◽  
Disease Course ◽  
Conjugated Bilirubin ◽  
Covalently Bound

Abstract At least four bilirubin fractions can be separated and identified by liquid chromatography, the least understood being the "delta" fraction (B delta), which apparently is covalently bound to albumin. To learn more about the incidence and significance of B delta, we assayed serum from 539 infants and children, both by the chromatographic method and the routine colorimetric Jendrassik-Grof method. The proportion of B delta appeared to correlate with both age and disease course. For infants younger than 28 days B delta generally was less than 2% of total bilirubin; for hyperbilirubinemic older infants and children the median B delta value was 35%. High B delta (greater than 50% of total bilirubin) in newborns was associated with intra- and extra-hepatic cholestasis, biliary cirrhosis, biliary atresia, and hepatitis. Among older infants and children, a proportion of low B delta (less than 10%) was found in hemolytic anemias, sepsis, shock, and other non-hepatic jaundice. In several cases, when low B delta was accompanied by increased conjugated bilirubin, the prognosis was very poor. Delayed clearance of B delta from the circulation together with its reactivity in direct diazo methods may interfere with interpretation of values for conjugated bilirubin as measured by classical methods.

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