Thin-layer chromatographic determination of urinary excretion of lactulose, simplified and applied to cystic fibrosis patients.

1987 ◽  
Vol 33 (7) ◽  
pp. 1211-1212 ◽  
Author(s):  
J A Flick ◽  
R L Schnaar ◽  
J A Perman
Keyword(s):  
Cystic Fibrosis ◽  
Oral Administration ◽  
Thin Layer ◽  
Urinary Excretion ◽  
Healthy Subjects ◽  
Chromatographic Determination ◽  
Solvent System ◽  
Chromatographic Technique ◽  
High Reproducibility

Abstract Urinary excretion of orally administered lactulose is used as an index of intestinal permeability. We have developed a simple thin-layer chromatographic technique for measuring lactulose in urine, using silica gel 60 plates and a propanol-borate solvent system. Lactulose concentrations as low as 62.5 mg/L can be detected with high reproducibility and without interference by urinary chromogens. After oral administration, the urinary excretion of lactulose in 8 h equaled 2.33 (SD 1.86)% in 15 patients with cystic fibrosis, as compared with 0.13 (SD 0.12)% in 16 healthy subjects (P less than 0.001).

High-performance thin-layer chromatographic determination of rosiglitazone in its dosage form

2002 ◽  
Vol 15 (3) ◽  
pp. 192-195 ◽  
Author(s):  
Ramesh Sane ◽  
Mary Francis ◽  
Atul Moghe ◽  
Sachin Khedkar ◽  
Ajit Anerao
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Dosage Form ◽  
Chromatographic Determination

Improved thin-layer chromatographic determination of phospholipids in gastric aspirate from newborns, for assessment of lung maturity.

1988 ◽  
Vol 34 (4) ◽  
pp. 736-738 ◽  
Author(s):  
D Serrano de la Cruz ◽  
E Santillana ◽  
A Mingo ◽  
G Fuenmayor ◽  
A Pantoja ◽  
...  
Keyword(s):  
Thin Layer ◽  
Fetal Lung ◽  
Chromatographic Determination ◽  
Solvent Mixture ◽  
Gastric Aspirate ◽  
One Dimensional ◽  
Acetic Acid Water ◽  
Chromatographic Resolution ◽  
Lung Maturity

Abstract This one-dimensional thin-layer chromatographic method is used for assay of phospholipids in the gastric aspirate of newborns. The solvent mixture (chloroform/hexane/methanol/glacial acetic acid/water, 12/7/4/3/0.3 by vol) completely resolves lecithin, sphingomyelin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, and phosphatidylglycerol. The method is simple, precise, inexpensive, and rapid (chromatographic development takes less than 25 min) and gives high chromatographic resolution. We used this method to determine the lecithin/sphingomyelin densitometric ratio (L/S ratio) and the phosphatidylglycerol percentage in 200 samples of gastric aspirate and found an L/S ratio of 2.5 to be a satisfactory cutoff value for distinguishing fetal lung maturity and immaturity. We confirmed that the presence of phosphatidylglycerol excluded the possibility of respiratory distress.

Gas Chromatographic Determination of Ametryn and Its Metabolites in Tropical Root Crops

1984 ◽  
Vol 67 (2) ◽  
pp. 280-284
Author(s):  
Promode C Bardalaye ◽  
Willis B Wheeler
Keyword(s):  
Anhydrous Sodium Sulfate ◽  
Residue Analysis ◽  
Gel Permeation Chromatography ◽  
Chromatographic Determination ◽  
Solvent System ◽  
Complete Separation ◽  
Flame Photometric Detector ◽  
Root Crops ◽  
Photometric Detector

Abstract Residue analysis of the herbicide ametryn (2-methyIthio-4-ethylamino-s-isopropylamino-s-triazine) is widely known but an analytical method for determining its metabolites has not yet been reported in the literature. A method has been developed for the extraction and determination of ametryn and 3 metabolites, 2-methylthio-4-amino-6-isopropylammo- s-triazine (GS-11354), 2-methylthio-4,6-diamino-.s-triazine(GS- 26831), and 2-methylthio-4-amino-6-ethylamino-s-triazine (GS-11355) in taniers, yams, cassava. Residues were extracted from crops with ethyl acetate-toluene (3 + 1 v/v), using a Polytron homogenizer and anhydrous sodium sulfate added for drying. The extracts were cleaned up by automated gel permeation chromatography on Bio-Beads SX-3 gel in the same solvent system. Quantitative determination was performed by gas chromatographic (GC) analysis on a column packed with 5% DEGS-PS on 100-120 mesh Supelcoport using either an N-P detector or a flame photometric detector (FPD) in the sulfur mode. Minimum detection by the flame photometric detector is 10 ng each for ametryn, GS-11354, and GS-11355 and 21 ng for GS-26831; by the N-P detector, 0.3 ng of each component gives easily quantitatable peaks. On a parts per million basis, starting with 25 g sample, the FPD detected a minimum level of 0.04 p.g/g each for ametryn, GS-11354, and GS-11355, and 0.08 p.g/g for GS-26831. The N-P detector could detect 0.0024 p.g/g for all 4 compounds. In addition to superior sensitivity, instrumental conditions allowed the complete separation of components in 10 min, for the N-P detector; more than 30 min was required for the FPD. Recoveries from fortified crops ranged from 67 to 111% at levels of 0.1-1.0 μg/g.

Quantitative thin-layer chromatographic determination of bromide(1-) residues in crops after soil treatment by methyl bromide

1979 ◽  
Vol 27 (1) ◽  
pp. 132-134 ◽  
Author(s):  
Luc A. Gordts ◽  
Andre Vandezande ◽  
Pieter P. Van Cauwenberge ◽  
Willy Van Haver
Keyword(s):  
Thin Layer ◽  
Methyl Bromide ◽  
Chromatographic Determination ◽  
Soil Treatment
Sign in / Sign up

Export Citation Format

Share Document