Direct determination of selenium in serum by graphite-furnace atomic absorption spectrometry with deuterium background correction and a reduced palladium modifier: age-specific reference ranges.

1988 ◽  
Vol 34 (4) ◽  
pp. 709-714 ◽  
Author(s):  
B E Jacobson ◽  
G Lockitch
Keyword(s):  
Atomic Absorption ◽  
Direct Method ◽  
Graphite Furnace ◽  
Direct Determination ◽  
Background Correction ◽  
Specific Reference ◽  
Reference Ranges ◽  
Term Infants ◽  
Graphite Furnace Atomic Absorption

Abstract We describe the development of a direct method for determination of selenium in serum by graphite-furnace atomic absorption spectrophotometry with deuterium background correction. We include palladium modifier to stabilize selenium in the presence of a strong reducing agent. Spectral interferences from iron are not evident in this system. Because an analysis requires only 20 microL of serum or plasma, the method is applicable to neonatal populations. At a selenium concentration of 0.75 mumol/L (approximately the mean found in our pre-term infants), the within-run CV is 5.3%. For a concentration of 1.83 mumol/L, which approximates our normal adult mean, within-run and between-run CVs are 2.7% and 3.4%. Accuracy is demonstrated by analytical recoveries ranging from 96% to 102%. We present reference values for pre-term and term infants, and age-specific ranges for infants to adults.

scholarly journals Determination of Lead in Croatian Wines by Graphite Furnace Atomic Absorption Spectrometry

2011 ◽  
Vol 62 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Blanka Tariba ◽  
Alica Pizent ◽  
Zorana Kljaković-Gašpić
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Graphite Furnace ◽  
Accurate Determination ◽  
Direct Determination ◽  
Absorption Spectrometry ◽  
White Wine ◽  
Matrix Modifier ◽  
Graphite Furnace Atomic Absorption

Determination of Lead in Croatian Wines by Graphite Furnace Atomic Absorption SpectrometryA method has been developed for direct determination of lead in wine by graphite furnace atomic absorption spectrometry (GFAAS) with Zeeman-effect background correction. The thermal behaviour of Pb during pyrolysis and atomisation stages was investigated without matrix modifier and in the presence of Pd(NO3)2, Pd(NO3)2+ Mg(NO3)2× 6H2O, and NH4H2PO4+ Mg(NO3)2× 6H2O as matrix modifiers. A simple 1:1 dilution of wine samples with Pd(NO3)2as a matrix modifier proved optimal for accurate determination of Pb in wine. Mean recoveries were 106 % for red and 114 % for white wine, and the detection limit was 3 μg L-1. Within-run precision of measurements for red and white wine was 2.1 % and 1.8 %, respectively. The proposed method was applied for analysis of 23 Croatian wines. Median Pb concentrations were 33 μg L-1, range (16 to 49) μg L-1in commercially available wines and 46 μg L-1, range (14 to 559) μg L-1in home-made wines. There were no statistically significant differences (P<0.05) in Pb concentration between commercial and home-made wines or between red and white wines.

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