A sensitive liquid-chromatographic method for determination of 3'-azido-3'-deoxythymidine (AZT) in plasma and urine.

1988 ◽  
Vol 34 (8) ◽  
pp. 1565-1568 ◽  
Author(s):  
M A Hedaya ◽  
R J Sawchuk
Keyword(s):  
Human Plasma ◽  
Prescription Drugs ◽  
Chromatographic Method ◽  
Internal Standard ◽  
Infectious Complications ◽  
Over The Counter ◽  
Internal Standards ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract We describe a liquid-chromatographic assay for AZT in human plasma and urine. This assay involves the use of two internal standards, allowing reference of AZT peaks to the appropriate internal standard, the choice depending on the range of concentrations encountered. This method is isocratic, specific, sensitive enough to allow quantification of AZT in concentrations observed clinically, and requires only 13 min of chromatographic time. We saw no interference from various over-the-counter and prescription drugs often used in treating the infectious complications of AIDS.

Liquid chromatographic method for the determination of rosiglitazone in human plasma

2003 ◽  
Vol 788 (1) ◽  
pp. 37-44 ◽  
Author(s):  
B.L Kolte ◽  
B.B Raut ◽  
A.A Deo ◽  
M.A Bagool ◽  
D.B Shinde
Keyword(s):  
Human Plasma ◽  
Chromatographic Method ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Gas-Liquid Chromatographic Determination of T-2 Toxin in Plasma

1983 ◽  
Vol 66 (4) ◽  
pp. 909-912 ◽  
Author(s):  
Steven P Swanson ◽  
Venkatachalam Ramaswamy ◽  
Val R Beasley ◽  
William B Buck ◽  
Harold H Burmeister
Keyword(s):  
Chromatographic Method ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Aqueous Sodium Hydroxide ◽  
Chromatographic Determination ◽  
Florisil Column ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract The gas-liquid chromatographic method for the determination of T-2 toxin in plasma is described. The toxin is extracted with benzene, washed with aqueous sodium hydroxide, and chromatographed on a small Florisil column; the heptafluorobutyryl derivative is prepared by reaction with heptafluorobutyrylimidazole. The T-2 HFB derivative is chromatographed onOV-1 at 230°C and measured with an electron capture detector. Iso-T-2, an isomer of T-2 toxin, is added to samples as an internal standard before extraction. Recoveries averaged 98.0 ± 5.5% at levels ranging from 50 to 1000 ng/m L. The limit of detection is 25 ng/mL.

scholarly journals Planar Chromatographic and Liquid Chromatographic Analysis of Thiamphenicol in Bovine and Human Plasma

1997 ◽  
Vol 80 (4) ◽  
pp. 746-750 ◽  
Author(s):  
Elena Dreassi ◽  
Gianfranco Corbini ◽  
Vittoria Ginanneschi ◽  
Piero Corti ◽  
Sandra Furlanetto
Keyword(s):  
Human Plasma ◽  
Chromatographic Analysis ◽  
Extraction Method ◽  
Chromatographic Method ◽  
Internal Standard ◽  
Bovine Plasma ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic ◽  
Pc Method

Abstract A planar chromatographic (PC) method was developed to determine thiamphenicol in human and bovine plasma with florphenicol as internal standard. The performance of the method was compared with that of a liquid chromatographic method. Recovery of extraction method developed for plasma was 81.51 ± 2.85%. Reproducibilities of the PC assay performed with various PC plates were also good.

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