scholarly journals Ultraviolet-absorbing organic anions in uremic serum separated by capillary zone electrophoresis, and quantification of hippuric acid

1990 ◽  
Vol 36 (3) ◽  
pp. 435-440 ◽  
Author(s):  
A C Schoots ◽  
T P Verheggen ◽  
P M De Vries ◽  
F M Everaerts
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Hippuric Acid ◽  
Sample Pretreatment ◽  
Organic Anions ◽  
Normal Serum ◽  
Analysis Time ◽  
Serum Samples ◽  
Uremic Serum ◽  
Zone Electrophoresis ◽  
Capillary Zone

Abstract Organic anions accumulated in blood serum of patients with chronic renal failure were separated by a novel technique: closed-system capillary zone electrophoresis (CZE) in a pH6 carrier-electrolyte system. Hippuric acid (HA), p-hydroxyhippuric acid, and uric acid were identified by their co-elution with standards prepared in ultrafiltered normal serum and by comparison with the corresponding ultraviolet-detected peaks positively identified in the HPLC analyses. Analysis time for the entire profile is 8 min. Repeatabilities (CVs) of CZE migration times and peak areas of the three acids in serum samples were about 0.7% and 6%, respectively. We quantified HA in 10 ultrafiltered uremic serum samples and compared results with those by a previously described HPLC procedure. The very good agreement further supports the identification of hippuric acid. Accuracy and precision of the CZE method were similar to those for the HPLC gradient-elution method, but analysis time for HA (8 min) is much less than by HPLC (90 min). Our technique is very suitable for selective, rapid analysis for (ultraviolet-absorbing) anionic constituents in ultrafiltered uremic serum, without any sample pretreatment.

scholarly journals Determination of Carbohydrate-deficient Transferrin Using Capillary Zone Electrophoresis

2001 ◽  
Vol 47 (2) ◽  
pp. 247-255 ◽  
Author(s):  
Birgitte Wuyts ◽  
Joris R Delanghe ◽  
Ishmael Kasvosve ◽  
Annick Wauters ◽  
Hugo Neels ◽  
...  
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Sample Pretreatment ◽  
Fused Silica ◽  
Exchange Chromatography ◽  
Buffer System ◽  
Good Precision ◽  
Deming Regression ◽  
Zone Electrophoresis ◽  
Carbohydrate Deficient Transferrin ◽  
Capillary Zone

Abstract Background: Current methods for carbohydrate-deficient transferrin (CDT) often suffer from low precision, complexity, or risk of false positives attributable to genetic variants. In this study, a new capillary zone electrophoresis (CZE) method for CDT was developed. Methods: CZE was performed on a P/ACE 5000 using fused-silica capillaries [50 μm (i.d.) × 47 cm] and the CEOFIX CDT buffer system with addition of 50 μL of anti-C3c and 10 μL of anti-hemoglobin. Native sera were loaded by high-pressure injection for 3 s, separated at 28 kV over 12 min, and monitored at 214 nm. Results: CDT was completely resolved by differences in migration times (di-trisialotransferrin, 9.86 ± 0.05 min; monosialotransferrin, 9.72 ± 0.05 min; asialotransferrin, 9.52 ± 0.04 min), with a CV of 0.15%. The number of theoretical plates was 312 000 ± 21 000 for the mono- and 199 000 ± 6500 for the di-trisialylated transferrin. Genetic CB and CD variants showed prominent peaks with migration times of 10.12 ± 0.06 and 9.89 ± 0.03 min, respectively, and the carbohydrate-deficient glycoprotein syndrome could be detected, excluding false-positive results. CZE results (as a percentage; y) correlated with the Axis %CDT TIATM (x) values by Deming regression analysis: y = 1.92x − 7.29; r = 0.89. CDT values in 130 healthy nonalcoholics were determined. The 2.5th and 97.5th percentiles were 1.84% and 6.79%. Conclusions: CZE without sample pretreatment can determine CDT with good precision, allows detection of variants, and correlates with ion-exchange chromatography.

Rapid optimized separation of bromide in serum samples with capillary zone electrophoresis by using glycerol as additive to the background electrolyte

2009 ◽  
Vol 1216 (15) ◽  
pp. 3349-3352 ◽  
Author(s):  
Jennifer P. Pascali ◽  
Eloisa Liotta ◽  
Rossella Gottardo ◽  
Federica Bortolotti ◽  
Franco Tagliaro
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Background Electrolyte ◽  
Serum Samples ◽  
Zone Electrophoresis ◽  
Capillary Zone

A NEW CAPILLARY ZONE ELECTROPHORESIS METHOD FOR THE SCREENING OF CONGENITAL DISORDERS OF GLYCOSYLATION (CDG)

2009 ◽  
Vol 32 (6S) ◽  
pp. 7
Author(s):  
F Parente ◽  
N Ah Mew ◽  
J Jaeken ◽  
B M Gilfix
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Metabolic Diseases ◽  
Clinical Syndrome ◽  
Congenital Disorders ◽  
Congenital Disorders Of Glycosylation ◽  
Serum Samples ◽  
Significant Difference ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Guthrie Cards

Background: The Congenital Disorders of Glycosylation (CDG) are an expanding group of metabolic diseases with a broad clinical presentation. We sought to validate a new Capillary Zone Electrophoresis (CZE) method (Sebia CAPILLARYS™ CDT) to screen for CDG. Methods: We analyzed 119 serum samples from children of varying ages and of both sexes to establish a reference range of transferrin glycoforms including CDT (Carbohydrate Deficient Transferrin). We then studied serums from 8 known CDG patients and compared the CZE results to the isoelectric focusing (IEF) profiles. We also analyzed serums after extraction from spotted Guthrie cards. Results: The mean (SD) percentage of transferrin glycoforms is 18.5 (4.4), 78.5 (4.2), 2.5 (1.3) and 0.6 (0.3) for penta-, tetra-, trisialotransferrin and CDT, respectively. There is no statistically significant difference between the different age groups analyzed (0-5, 6-11, 12-15, 16-18, and > 18 years) or between sexes. We observed a good correlation between the CZE and IEF profiles with both fresh serum and serum extracted from Guthrie cards. Conclusions: The Sebia CAPILLARYS™ CDT system is a simple and reliable method to screen for CDG in pediatric and adult patients with an unexplained clinical syndrome, particularly when the nervous system is involved.

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