Rapid optimized separation of bromide in serum samples with capillary zone electrophoresis by using glycerol as additive to the background electrolyte

2009 ◽  
Vol 1216 (15) ◽  
pp. 3349-3352 ◽  
Author(s):  
Jennifer P. Pascali ◽  
Eloisa Liotta ◽  
Rossella Gottardo ◽  
Federica Bortolotti ◽  
Franco Tagliaro
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Background Electrolyte ◽  
Serum Samples ◽  
Zone Electrophoresis ◽  
Capillary Zone

scholarly journals Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Thi Thanh Vuong Tong ◽  
Thi Thoa Cao ◽  
Nguyen Ha Tran ◽  
Thi Kim Van Le ◽  
Dinh Chi Le
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Background Electrolyte ◽  
Cost Effective ◽  
Complete Separation ◽  
Sodium Tetraborate ◽  
Eye Drops ◽  
Solution Ph ◽  
Zone Electrophoresis ◽  
Capillary Zone

A green, cost-effective, and simple capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of chloramphenicol, methylparaben, and propylparaben in eye-drops. With sodium tetraborate as background electrolyte (BGE), the apparent mobilities of chloramphenicol, methylparaben, and propylparaben increased and analysis time reduced when pH of BGE increased from 8.5 to 10.0 and concentration of BGE decreased from 40 mM to 15 mM, but complete separation of chloramphenicol from other matrix components was achieved only with sodium tetraborate concentration at 30 mM or higher and at pH = 9.3 or lower. The most suitable electrophoretic conditions for the intended application were a 30 mM sodium tetraborate solution, pH 9.3 as BGE, working voltage set at 25 kV, and UV detection at 280 nm at the cathodic extremity of the capillary. The final method was validated and proved to be reliable for assay of chloramphenicol, methylparaben, and propylparaben in eye-drops.

scholarly journals Ultraviolet-absorbing organic anions in uremic serum separated by capillary zone electrophoresis, and quantification of hippuric acid

1990 ◽  
Vol 36 (3) ◽  
pp. 435-440 ◽  
Author(s):  
A C Schoots ◽  
T P Verheggen ◽  
P M De Vries ◽  
F M Everaerts
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Hippuric Acid ◽  
Sample Pretreatment ◽  
Organic Anions ◽  
Normal Serum ◽  
Analysis Time ◽  
Serum Samples ◽  
Uremic Serum ◽  
Zone Electrophoresis ◽  
Capillary Zone

Abstract Organic anions accumulated in blood serum of patients with chronic renal failure were separated by a novel technique: closed-system capillary zone electrophoresis (CZE) in a pH6 carrier-electrolyte system. Hippuric acid (HA), p-hydroxyhippuric acid, and uric acid were identified by their co-elution with standards prepared in ultrafiltered normal serum and by comparison with the corresponding ultraviolet-detected peaks positively identified in the HPLC analyses. Analysis time for the entire profile is 8 min. Repeatabilities (CVs) of CZE migration times and peak areas of the three acids in serum samples were about 0.7% and 6%, respectively. We quantified HA in 10 ultrafiltered uremic serum samples and compared results with those by a previously described HPLC procedure. The very good agreement further supports the identification of hippuric acid. Accuracy and precision of the CZE method were similar to those for the HPLC gradient-elution method, but analysis time for HA (8 min) is much less than by HPLC (90 min). Our technique is very suitable for selective, rapid analysis for (ultraviolet-absorbing) anionic constituents in ultrafiltered uremic serum, without any sample pretreatment.

A Capillary Zone Electrophoresis Method for Analysis of Glycosylated Recombinant Human Follicle-Stimulating Hormone

2019 ◽  
Vol 35 (4) ◽  
pp. 55-64
Author(s):  
M.E. Sushkin ◽  
A.S. Simbirtsev
Keyword(s):  
Sodium Chloride ◽  
Capillary Zone Electrophoresis ◽  
Protein Separation ◽  
Follicle Stimulating Hormone ◽  
Background Electrolyte ◽  
Sialic Acids ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone ◽  
Stimulating Hormone

Conditions for analyzing sialylated isoforms of recombinant follicle-stimulating hormone (FSH) by the method of capillary zone electrophoresis have been selected. Follicle-stimulating hormone is a glycosylated heterodimer with the large number of sialic acids that affect its basic biological and physicochemical properties. The optimum protein separation into 6 isoforms was achieved using as a background electrolyte of a solution with pH 4.3 containing 0.01 M trycin, 0.01 sodium chloride, 7 M urea and 2.5 mM 1,4-diaminobutane. follicle-stimulating hormone isoforms, capillary zone electrophoresis, sialic acids, follicle-stimulating hormone.

A NEW CAPILLARY ZONE ELECTROPHORESIS METHOD FOR THE SCREENING OF CONGENITAL DISORDERS OF GLYCOSYLATION (CDG)

2009 ◽  
Vol 32 (6S) ◽  
pp. 7
Author(s):  
F Parente ◽  
N Ah Mew ◽  
J Jaeken ◽  
B M Gilfix
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Metabolic Diseases ◽  
Clinical Syndrome ◽  
Congenital Disorders ◽  
Congenital Disorders Of Glycosylation ◽  
Serum Samples ◽  
Significant Difference ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Guthrie Cards

Background: The Congenital Disorders of Glycosylation (CDG) are an expanding group of metabolic diseases with a broad clinical presentation. We sought to validate a new Capillary Zone Electrophoresis (CZE) method (Sebia CAPILLARYS™ CDT) to screen for CDG. Methods: We analyzed 119 serum samples from children of varying ages and of both sexes to establish a reference range of transferrin glycoforms including CDT (Carbohydrate Deficient Transferrin). We then studied serums from 8 known CDG patients and compared the CZE results to the isoelectric focusing (IEF) profiles. We also analyzed serums after extraction from spotted Guthrie cards. Results: The mean (SD) percentage of transferrin glycoforms is 18.5 (4.4), 78.5 (4.2), 2.5 (1.3) and 0.6 (0.3) for penta-, tetra-, trisialotransferrin and CDT, respectively. There is no statistically significant difference between the different age groups analyzed (0-5, 6-11, 12-15, 16-18, and > 18 years) or between sexes. We observed a good correlation between the CZE and IEF profiles with both fresh serum and serum extracted from Guthrie cards. Conclusions: The Sebia CAPILLARYS™ CDT system is a simple and reliable method to screen for CDG in pediatric and adult patients with an unexplained clinical syndrome, particularly when the nervous system is involved.

Sign in / Sign up

Export Citation Format

Share Document