Identification of an ascorbic acid metabolite among "uremic middle molecules"

1990 ◽  
Vol 36 (7) ◽  
pp. 1369-1372 ◽  
Author(s):  
P M Gallice ◽  
J P Monti ◽  
D L Braguer ◽  
H M Monti ◽  
R E Elsen ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Ascorbic Acid ◽  
Mass Range ◽  
Inhibitory Effect ◽  
Tubulin Polymerization ◽  
Acid Metabolite ◽  
13C Nuclear Magnetic Resonance ◽  
Middle Molecules ◽  
Microtubule Formation ◽  
Main Component

Abstract Among uremic toxins in the middle molecular mass range, 1H, 13C-nuclear magnetic resonance, ultraviolet spectrometry, and chromatographic analyses allow identification of the main component of the so-called "2-5-3 fraction" as ascorbic acid 2-sulfate, a conjugated metabolite of ascorbic acid. We previously (Clin Nephrol 1986;25:212-8) showed an inhibitory effect of the 2-5-3 fraction on microtubule formation. Therefore, we tested the action of ascorbic acid 2-sulfate and its synthetized enantiomers on tubulin polymerization. Because these molecules did not exert any inhibitory effect, we hypothesize that the 2-5-3 fraction is a mixture of compounds in which only a very low quantity of the inhibitory factor is present.

A compound from uremic plasma and from normal urine isolated by liquid chromatography and identified by nuclear magnetic resonance.

1985 ◽  
Vol 31 (1) ◽  
pp. 30-34 ◽  
Author(s):  
P Gallice ◽  
J P Monti ◽  
A Crevat ◽  
C Durand ◽  
A Murisasco
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
Isolation And Identification ◽  
13C Nuclear Magnetic Resonance ◽  
Middle Molecules ◽  
Nuclear Magnetic Resonance Spectrometry ◽  
Magnetic Resonance Spectrometry ◽  
Normal Urine ◽  
Nuclear Magnetic

Abstract A compound present in normal urine and in ultrafiltrates of uremic plasma in the fraction of so-called "uremic middle molecules" was isolated by liquid chromatography. Preliminary studies, including amino acid analysis, characterization of uronic acids, and ultraviolet spectroscopy, show that the molecule contains glycine, a uronic acid, and an aromatic ring. Characterization by 1H and 13C nuclear magnetic resonance spectrometry shows conclusively that this compound is a double conjugate of glucuronidate--o-hydroxyhippuric acid, which has been previously described by Zimmerman et al., using quite different techniques of isolation and identification (Clin Nephrol 14: 107, 1980; FEBS Lett 129: 237, 1981).

Identification by nuclear magnetic resonance and mass spectrometry of a glucuronic acid conjugate of o-hydroxybenzoic acid in normal urine and uremic plasma.

1985 ◽  
Vol 31 (10) ◽  
pp. 1640-1643 ◽  
Author(s):  
J P Monti ◽  
P Gallice ◽  
A Crevat ◽  
A Murisasco
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Glucuronic Acid ◽  
Hydroxybenzoic Acid ◽  
13C Nuclear Magnetic Resonance ◽  
Middle Molecules ◽  
Endogenous Compound ◽  
Normal Urine ◽  
Nuclear Magnetic

Abstract An endogenous compound (included in the fraction of uremic toxins often called the "uremic middle molecules") was separated from plasma of uremic patients and urine from normal persons. As elucidated by mass spectrometry, enzymatic hydrolysis, and 1H, 13C nuclear magnetic resonance, it appears to be a conjugate of o-hydroxybenzoic acid with glucuronic acid. Its presence in urine of healthy subjects indicates its physiological character.

Inhibitory Effect of Ascorbic Acid 2-O-.ALPHA.-Glucoside on the Pigmentation of Skin by Exposure to Ultraviolet Light.

1996 ◽  
Vol 58 (3) ◽  
pp. 439-443 ◽  
Author(s):  
Eriko MIYAI ◽  
Itaru YAMAMOTO ◽  
Jun-ichi AKIYAMA ◽  
Mitsuhiro YANAGIDA
Keyword(s):  
Ascorbic Acid ◽  
Ultraviolet Light ◽  
Inhibitory Effect

13C nuclear magnetic resonance study of cyclodipeptides containing 13C enriched hydrophobic amino acids

1980 ◽  
Vol 45 (2) ◽  
pp. 482-490 ◽  
Author(s):  
Jaroslav Vičar ◽  
François Piriou ◽  
Pierre Fromageot ◽  
Karel Bláha ◽  
Serge Fermandjian
Keyword(s):  
Amino Acids ◽  
Nuclear Magnetic Resonance ◽  
Nuclear Magnetic Resonance Study ◽  
Coupling Constants ◽  
Side Chain ◽  
Amino Acid Residues ◽  
Magnetic Resonance Study ◽  
Side Chain Conformation ◽  
13C Nuclear Magnetic Resonance ◽  
Hydrophobic Amino Acids

The diastereoisomeric pairs of cyclodipeptides cis- and trans-cyclo(Ala-Ala), cyclo(Ala-Phe), cyclo(Val-Val) and cyclo(Leu-Leu) containing 85% 13C enriched amino-acid residues were synthesized and their 13C-13C coupling constants were measured. The combination of 13C-13C and 1H-1H coupling constants enabled to estimate unequivocally the side chain conformation of the valine and leucine residues.

scholarly journals Inhibitory effect of the Ascorbic Acid on photodegradation of pharmaceuticals compounds exposed to UV-B radiation

2021 ◽  
pp. 100035
Author(s):  
Consuelo León ◽  
Claudio Henríquez ◽  
Nicolás López ◽  
Georgina Sanchez ◽  
Bárbara Pastén ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Inhibitory Effect
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