Mitogens for adult rat aortic vascular smooth muscle cells in serum-free primary culture

1994 ◽  
Vol 28 (8) ◽  
pp. 1238-1242 ◽  
Author(s):  
D. J Grainger ◽  
C. M Witchell ◽  
P. L Weissberg ◽  
J. C Metcalfe
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Primary Culture ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Serum Free ◽  
Adult Rat

Motor responses of cultured rat cerebral vascular smooth muscle cells to intra- and extracellular pH changes

1997 ◽  
Vol 273 (1) ◽  
pp. H434-H445 ◽  
Author(s):  
M. Apkon ◽  
R. A. Weed ◽  
W. F. Boron
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Extracellular Ph ◽  
Deformation Pattern ◽  
Motor Responses ◽  
Ph Changes ◽  
Adult Rat

Alkalizing perivascular fluid constricts, whereas acidification dilates, cerebral arterioles. It is not known whether vascular smooth muscle cells (VSMCs), endothelium, or neuronal elements sense pH changes. We hypothesized that VSMCs themselves transduce extracellular pH (pHo) changes. We examined the motor responses of cultured adult rat middle cerebral arterial VSMCs during pHo and intracellular pH (pHi) changes. Motor responses were inferred from the deformation pattern of a silicone substratum, dimethylpolysiloxane, which wrinkles as cells contract. pHi was measured with 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. Cultured VSMCs retained motor responses to vasoconstrictors (5-hydroxytryptamine and K+), and to sodium nitroprusside, which were typical of intact arterioles. VSMCs contracted with increasing and relaxed with decreasing pHo. Hypocapnia contracted VSMCs when the pHo increased, and hypercapnia relaxed VSMCs when the pHo decreased. However, at a constant pHo, changes in PCO2 caused opposite responses despite equivalent changes in pHi. Thus VSMCs contract with increased pHo and relax with decreased pHo just as intact arterioles do. These responses do not reflect changes in pHi or PCO2. pHi changes paradoxically alter VSMC tone in the direction opposite that caused by pHo changes.

scholarly journals Substance-specific importance of EGFR for vascular smooth muscle cells motility in primary culture

2016 ◽  
Vol 1863 (7) ◽  
pp. 1519-1533 ◽  
Author(s):  
Barbara Schreier ◽  
Gerald Schwerdt ◽  
Christian Heise ◽  
Daniel Bethmann ◽  
Sindy Rabe ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Primary Culture ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells
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