scholarly journals IsoXpressor: A Tool to Assess Transcriptional Activity within Isochores

2020 ◽  
Vol 12 (9) ◽  
pp. 1573-1578
Author(s):  
Lorraine A K Ayad ◽  
Athanasia-Maria Dourou ◽  
Stilianos Arhondakis ◽  
Solon P Pissis

Abstract Genomes are characterized by large regions of homogeneous base compositions known as isochores. The latter are divided into GC-poor and GC-rich classes linked to distinct functional and structural properties. Several studies have addressed how isochores shape function and structure. To aid in this important subject, we present IsoXpressor, a tool designed for the analysis of the functional property of transcription within isochores. IsoXpressor allows users to process RNA-Seq data in relation to the isochores, and it can be employed to investigate any biological question of interest for any species. The results presented herein as proof of concept are focused on the preimplantation process in Homo sapiens (human) and Macaca mulatta (rhesus monkey).

CYTOLOGIA ◽  
2006 ◽  
Vol 71 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Alongkoad Tanomtong ◽  
Sumpars Khunsook ◽  
Aunrat Chaveerach ◽  
Wiwat Kaensa ◽  
Ruangvit Banjongrat

2018 ◽  
Vol 50 (8) ◽  
pp. 628-635 ◽  
Author(s):  
Uros Midic ◽  
Catherine A. VandeVoort ◽  
Keith E. Latham

To account for sex as a biological variable, it is sometimes necessary to identify the sex of an embryo or embryonic cell that was used to generate libraries for RNA sequencing, without the sex being known a priori. The preferred approach for this would take advantage of the mRNA data, rather than relying on other methods that require separation and analysis of genomic DNA or diversion of limiting RNA for other assays. We describe here a method that has been optimized for this purpose in samples of rhesus monkey and mouse embryos. This method is broadly applicable to any species for which a sufficiently well characterized genome and knowledge of polymorphisms are available, and for embryos that are transcriptionally active and expressing their genome.


1976 ◽  
Vol 19 (3) ◽  
pp. 246-251 ◽  
Author(s):  
Helen H. Molinari ◽  
Andrew J. Rózsa ◽  
Dan R. Kenshalo

2021 ◽  
Vol 59 (1) ◽  
pp. 175-182
Author(s):  
Dong-hong Tang ◽  
Chen-yun Wang ◽  
Xi Huang ◽  
Hong-kun Yi ◽  
Zhe-li Li ◽  
...  

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