scholarly journals Interrelationships between seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation: implications for assisted conception

2001 ◽  
Vol 16 (10) ◽  
pp. 2160-2165 ◽  
Author(s):  
M.J. Tomlinson ◽  
O. Moffatt ◽  
G.C. Manicardi ◽  
D. Bizzaro ◽  
M. Afnan ◽  
...  
Keyword(s):  
Dna Damage ◽  
Density Gradient ◽  
Nuclear Dna ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Assisted Conception ◽  
Seminal Parameters ◽  
Before And After

scholarly journals ELECTRON MICROSCOPY OF LYSOSOME-RICH FRACTIONS FROM RAT THYMUS ISOLATED BY DENSITY-GRADIENT CENTRIFUGATION BEFORE AND AFTER WHOLE-BODY X-IRRADIATION

1962 ◽  
Vol 13 (2) ◽  
pp. 253-260 ◽  
Author(s):  
Y. E. Rahman
Keyword(s):  
Electron Microscopy ◽  
Acid Phosphatase ◽  
Cytochrome Oxidase ◽  
Density Gradient ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Whole Body ◽  
Rat Tissues ◽  
Before And After ◽  
X Irradiation

Fractions from rat thymuses were isolated by sucrose density-gradient centrifugation, before and after 1000 r whole-body x-irradiation, and examined by electron microscopy. Cytochrome oxidase and acid phosphatase activities of these fractions were tested as well. Electron-opaque bodies with diameters ranging from 0.10 to 0.35 µ, with a mean of 0.25 µ, were found in fractions having high acid phosphatase activity, while the fractions rich in cytochrome oxidase consisted mostly of mitochondria. After irradiation, there was an increased ratio of dense bodies to mitochondria. These particles are considered to be lysosomes similar to those identified in other rat tissues. Their relationship to the mitochondria is discussed.

scholarly journals Inter-center variation in the efficiency of sperm DNA damage reduction following density gradient centrifugation

2013 ◽  
Vol 05 (07) ◽  
pp. 15-20
Author(s):  
Carmen López-Fernández ◽  
Stephen D. Johnston ◽  
Altea Gosálbez ◽  
Jose Luís Fernández ◽  
Juan G. Álvarez ◽  
...  
Keyword(s):  
Dna Damage ◽  
Density Gradient ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Sperm Dna Damage ◽  
Damage Reduction ◽  
Sperm Dna
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