Frozen-thawed embryo transfer: influence of clinical factors on implantation rate and risk of multiple conception

BackgroundIn recent years frozen-thawed embryo transfer (FET) has played an increasingly important role in ART, but there is limited consensus on the most effective method of endometrial preparation (EP) for FET. Inspired by significantly higher implantation rate and clinical pregnancy rate of the depot GnRH-a protocol, we proposed a novel EP protocol named down-regulation ovulation-induction (DROI) aimed to improve pregnancy outcomes of FET.MethodsThis was a single-center, randomized controlled pilot trial. A total of 307 patients with freeze-all strategy scheduled for first FET were enrolled in the study. A total 261 embryos were transferred in DROI-FET group including 156 patients and 266 embryos were transferred in mNC-FET group including 151 patients. Reproductive outcomes were compared between the two groups.ResultsThe basic characteristics of patients, and the average number, quality and stage of embryos transferred were comparable between the two groups. Our primary outcome, implantation rate(IR) in DROI-FET group, was significantly higher than that of the mNC-FET group (54.41% versus 35.71%, P<0.01). The clinical pregnancy rate (CPR) and ongoing pregnancy rate (OPR) in DROI-FET group was also higher than that in mNC-FET group (69.87% versus 50.33%, P<0.01; 64.10% versus 42.38%, P<0.01).Conclusion(s)Compared to existing endometrial preparation methods, the DROI protocol might be the more efficient and promising protocol.

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Clinical factors that affect the pregnancy rate in frozen-thawed embryo transfer in the freeze-all policy

Yeungnam University Journal of Medicine ◽

10.12701/yujm.2019.00346 ◽

2020 ◽

Vol 37 (1) ◽

pp. 47-53

Author(s):

Seo Yoon Hwang ◽

Eun Hye Jeon ◽

Seung Chul Kim ◽

Jong Kil Joo

Keyword(s):

Pregnancy Rate ◽

Embryo Transfer ◽

Clinical Factors ◽

Frozen Thawed Embryo Transfer ◽

Thawed Embryo Transfer

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Impacts of Cyclosporin A on clinical pregnancy outcomes of patients with a history of unexplained transfer failure: a retrospective cohort study

Reproductive Biology and Endocrinology ◽

10.1186/s12958-021-00728-x ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Danni Qu ◽

Xiangming Tian ◽

Ling Ding ◽

Yuan Li ◽

Wenhui Zhou

Keyword(s):

Cyclosporin A ◽

Pregnancy Rate ◽

Embryo Transfer ◽

Pregnancy Outcomes ◽

Retrospective Cohort ◽

Implantation Rate ◽

Clinical Pregnancy ◽

History Of ◽

Frozen Thawed Embryo Transfer ◽

Thawed Embryo Transfer

Abstract Background A rapid development in assisted reproductive technology (ART) has led to a surge in its popularity among target couples. However, elucidation on the molecular mechanism and effective solutions for a common problem posed by ART, namely transfer failure, is still lacking. The new therapeutic potential of cyclosporin A (CsA), a typical immunosuppressant widely used in the treatment of rejection after organ transplantation, in recurrent pregnancy loss (RPL) patients may inspire some novel transfer failure therapies in the future. To further explore the clinical effects of CsA, this study investigated whether its application can improve clinical pregnancy outcomes in patients with a history of unexplained transfer failure in frozen-thawed embryo transfer (FET) cycles. Methods Data from a retrospective cohort investigation (178 frozen-thawed embryo transfer cycles in 178 patients) were analysed using binary logistic regression to explore the relationship between CsA treatment and clinical pregnancy outcomes; the odds ratios (ORs) and 95 % confidence intervals (CIs) were calculated as a measure of relevancy. Implantation rate was the main outcome measure. Results There was no difference in the fine adjusted OR (95 % CI) of the implantation rate [1.251 (0.739–2.120)], clinical pregnancy rate [1.634 (0.772–3.458)], chemical pregnancy rate [1.402 (0.285–6.909)], take-home baby rate [0.872 (0.423–1.798)], multiple births rate [0.840 (0.197–3.590)], preterm birth [1.668 (0.377–7.373)], abnormal birth weight [1.834 (0.533–6.307)] or sex ratio [0.956 (0.339–2.698)] between the CsA-treated group and control group. No birth defects were observed in the present study. Conclusions Although CsA does not affect infant characteristics, it has no beneficial effects on the clinical pregnancy outcomes in patients with a history of unexplained transfer failure in FET cycles.

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Comparison of birthweights in patients randomly assigned to fresh or frozen-thawed embryo transfer

10.26226/morressier.573c1513d462b80296c98a23 ◽

2016 ◽

Author(s):

Bruce Shapiro

Keyword(s):

Embryo Transfer ◽

Frozen Thawed Embryo Transfer ◽

Thawed Embryo Transfer

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THE EFFECT OF ASSISTED HATCHING IN FROZEN-THAWED EMBRYO TRANSFER CYCLES

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2016.3.12 ◽

2016 ◽

pp. 80-84

Author(s):

Thi Tam An Nguyen ◽

Minh Tam Le ◽

Ngoc Thanh Cao

Keyword(s):

Embryo Transfer ◽

Zona Pellucida ◽

Assisted Hatching ◽

Implantation Failure ◽

Multiple Pregnancies ◽

Recurrent Implantation Failure ◽

Key Words Laser ◽

Implantation And Pregnancy Rates ◽

Frozen Thawed Embryo Transfer ◽

Thawed Embryo Transfer

Background: Laser assisted hatching technique based on the hypothesis to make an artificial hole on zona pellucida (ZP) that can help embryo hatching out of ZP easily. This technique has been shown to increase implantation and pregnancy rates in women of advanced age, in women with recurrent implantation failure and following the transfer of frozen–thawed embryos. This study described the outcome of frozen–thawed embryo transfers with laser assisted hatching (LAH), which is one of the safest method in nowadays. Purpose: To assess the effect of assisted hatching technique on the clinical outcomes in vitrified-warmed transfer cycles. Method: A total of 65 thawed-transfer cycles with 153 thawed-embryos undertaken within a 12-month period were analysed, Assisted hatching with laser zona thinning was performed with one-quarter of the zona pellucida circumference. The overall thawed-embryos (day 3) were kept in culture overnight. Patient were prepared the suitable endometrium and transferred embryos advantageously. Results: In which, having the rate of survival embryos were 143 occupying 94.3%, the percentage of grade 1 and 2 embryos occupied 55.9% and 29,3% respectively, and that were enrolled LAH before transfering of frozen–thawed embryos. The average transferred embryos were 2.4±0.8, The rate of implantation per transferred embryos and per transferred embryos cycles was 19.5% and 43.1% respectively. The rate of clinical pregnancies per embryo transfer cycles occupied 33.8% with percentage of early miscarriages (biochemical pregnancies and early clinical miscarriages) was 12.3%. The rate of ongoing pregnancies was 30.8% and multiple pregnancies was low just 12.3%. This result was equal or higher than other researchs in embryos transfer had or no LAH. Conclusion: LAH contributed to stable frozen–thawed embryos transfer effectiveness. Key words: Laser assisted hatching, frozen–thawed embryos transfer, zona pellucida (ZP)

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