scholarly journals Fluorescent polymerase chain reaction: Part I. A new method allowing genetic diagnosis and DNA fingerprinting of single cells

1996 ◽  
Vol 2 (2) ◽  
pp. 137-152 ◽  
Author(s):  
I Findlay
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Fingerprinting ◽  
Single Cells ◽  
Genetic Diagnosis ◽  
New Method ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Fluorescent Polymerase Chain Reaction

scholarly journals Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells

1988 ◽  
Vol 16 (23) ◽  
pp. 10953-10971 ◽  
Author(s):  
Alec J. Jeffreys ◽  
Victoria Wilson ◽  
Rita Neumann ◽  
John Keyte
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Fingerprinting ◽  
Single Cells ◽  
Chain Reaction ◽  
Polymerase Chain

Rapid Aneuploidy Detection of Chromosomes 13, 18, 21, X and Y Using Quantitative Fluorescent Polymerase Chain Reaction with Few Microdissected Fetal Cells

2015 ◽  
Vol 38 (1) ◽  
pp. 65-76 ◽  
Author(s):  
Ahmed Emad ◽  
Josée Lamoureux ◽  
Annie Ouellet ◽  
Régen Drouin
Keyword(s):  
Polymerase Chain Reaction ◽  
Tandem Repeats ◽  
Single Cells ◽  
Pcr Analysis ◽  
Limiting Factor ◽  
Chain Reaction ◽  
Fetal Cells ◽  
Allele Dosage ◽  
Polymerase Chain ◽  
Fluorescent Polymerase Chain Reaction

Objectives: Analysis of DNA from small numbers of cells, such as fetal cells in maternal blood, is a major limiting factor for their use in clinical applications. Traditional methods of single-cells whole genome amplification (SCs-WGA) and accurate analysis have been challenging to date. Our purpose was to assess the feasibility of using a few fetal cells to determine fetal sex and major chromosomal abnormalities by quantitative fluorescent polymerase chain reaction (QF-PCR). Methods: Cultured cells from 26 amniotic fluid samples were used for standard DNA extraction and recovery of 5 fetal cells by laser-capture microdissection. SCs-WGA was performed using the DNA from the microdissected cells. PCR amplification of short tandem repeats specific for chromosomes 13, 18, 21, X and Y was performed on extracted and amplified DNA. Allele dosage and sexing were quantitatively analyzed following separation by capillary electrophoresis. Results: Microsatellite QF-PCR analysis showed high concordance in chromosomal copy number between extracted and amplified DNA when 5 or more cells were used. Results were in concordance with that of conventional cytogenetic analysis. Conclusion: Satisfactory genomic coverage can be obtained from SCs-WGA. Clinically, SCs-WGA coupled with QF-PCR can provide a reliable, accurate, rapid and cost-effective method for detection of major fetal chromosome abnormalities.

Detection of trisomy 21 by quantitative fluorescent-polymerase chain reaction in uncultured amniocytes

2003 ◽  
Vol 23 (4) ◽  
pp. 287-291 ◽  
Author(s):  
J�r�me Solassol ◽  
Ha�ssam Rahil ◽  
Vincent Sapin ◽  
Didier Lemery ◽  
Bernard Dastugue ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Trisomy 21 ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Fluorescent Polymerase Chain Reaction
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