scholarly journals Multiple Hepatitis B Virus (HBV) Quasispecies and Immune-Escape Mutations Are Present in HBV Surface Antigen and Reverse Transcriptase of Patients With Acute Hepatitis B

2016 ◽  
Vol 213 (12) ◽  
pp. 1897-1905 ◽  
Author(s):  
Marianna Aragri ◽  
Claudia Alteri ◽  
Arianna Battisti ◽  
Domenico Di Carlo ◽  
Carmine Minichini ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Reverse Transcriptase ◽  
Acute Hepatitis ◽  
Surface Antigen ◽  
Immune Escape ◽  
B Virus ◽  
Acute Hepatitis B

scholarly journals Improved Detection of Hepatitis B Virus Surface Antigen by a New Rapid Automated Assay

1999 ◽  
Vol 37 (8) ◽  
pp. 2639-2647 ◽  
Author(s):  
Bernard Weber ◽  
Anja Bayer ◽  
Peter Kirch ◽  
Volker Schlüter ◽  
Dietmar Schlieper ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Acute Hepatitis ◽  
Sensitivity And Specificity ◽  
Surface Antigen ◽  
Core Antigen ◽  
B Virus ◽  
Acute Hepatitis B ◽  
Screening Assays ◽  
Hbsag Screening

The performance of hepatitis B virus (HBV) surface antigen (HBsAg) screening assays is continuously improved in order to reduce the residual risk of transfusion-associated hepatitis B. In a multicenter study, a new automated rapid screening assay, Elecsys HBsAg (Roche Diagnostics), was compared to well-established tests (Auszyme Monoclonal [overnight incubation] version B and IMx HBsAg [Abbott]). Included in the evaluation were 23 seroconversion panels; sera from the acute and chronic phases of infection; dilution series of various HBsAg standards, HBV subtypes, and S gene mutants; and isolated anti-HBV core antigen-positive samples. To challenge the specificity of the new assay, sera from HBsAg-negative blood donors, pregnant women, and dialysis and hospitalized patients and potentially cross-reactive samples were investigated. Elecsys HBsAg showed a higher sensitivity for HBsAg subtypes ad, ay, adw2, adw4, ayw1, ayw2, ayw4, and adr detection in dilution series of different standards or sera than Auszyme Monoclonal version B and/or IMx HBsAg. Acute hepatitis B was detected in 11 to 16 of 23 seroconversion panels between 2 and 16 days earlier with Elecsys HBsAg than with the alternative assays. Elecsys HBsAg and Auszyme Monoclonal version B detected HBsAg surface mutants with equal sensitivity. The sensitivity and specificity of Elecsys HBsAg were 100%. Auszyme Monoclonal version B had a 99.9% specificity, and its sensitivity was 96.6%. IMx HBsAg showed a poorer sensitivity and specificity than the other assays. In conclusion, Elecsys HBsAg permits earlier detection of acute hepatitis B and different HBV subtypes than the alternative assays. By using highly sensitive HBsAg screening assays, low-level HBsAg carriers among isolated anti-HBV core antigen-positive individuals can be detected.

scholarly journals Hepatitis B virus DNA in serum and blood cells of hepatitis B surface antigen-negative hemodialysis patients and staff.

1997 ◽  
Vol 8 (9) ◽  
pp. 1443-1447
Author(s):  
M Cabrerizo ◽  
J Bartolomé ◽  
P De Sequera ◽  
C Caramelo ◽  
V Carreño
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Acute Hepatitis ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Hemodialysis Patients ◽  
Hbv Dna ◽  
Staff Members ◽  
B Virus ◽  
Acute Hepatitis B

Patients undergoing chronic hemodialysis, as well as dialysis staff members, are at high risk of infection with hepatitis B virus (HBV). We have analyzed by PCR the presence of HBV DNA in serum and peripheral blood mononuclear cells (PBMC) from 33 hepatitis B surface antigen (HBsAg)-negative hemodialysis patients and 24 dialysis unit staff members; eight of the 24 staff members had an acute hepatitis B resolved 13 to 21 yr before. HBV DNA was detected in serum of 19 (58%) patients (12 of 17 with and 7 of 16 without anti-HBV antibodies). HBV DNA was found in PBMC of 18 (54%) patients (13 of 17 with and 5 of 16 without anti-HBV antibodies). In the staff members, serum HBV DNA was found only in the individuals who suffered a previous acute hepatitis (P < 0.005). HBV DNA was detected in PBMC of four of six staff members (all with previous acute hepatitis). In two HBV DNA-positive PBMC samples, viral RNA was detected by reverse transcription-PCR. To ascertain whether the HBV DNA detected in serum was encapsulated, seven HBV DNA-positive serum samples were digested with DNase before PCR. After treatment, HBV DNA remained detectable in four cases. In conclusion, HBV DNA in serum and PBMC is detectable in a high proportion of HBsAg-negative hemodialysis patients and may persist several years after a resolved acute hepatitis B. The viral DNA is encapsulated and remains transcriptionally active in PBMC. In the anti-HBs-negative patients, HBV DNA is, at the present time, the only means for diagnosing a past HBV hepatitis.

scholarly journals Acute Acalculous Cholecystitis Induced by Acute Hepatitis B Virus Infection

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Riyadh Ali Mohammed ◽  
Wisam Ghadban ◽  
Osama Mohammed
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Acute Hepatitis ◽  
Rare Complication ◽  
Acalculous Cholecystitis ◽  
Acute Acalculous Cholecystitis ◽  
Hepatitis B Virus Infection ◽  
B Virus ◽  
Acute Hepatitis B

During the course of acute viral hepatitis, some functional and anatomical changes to the gallbladder can occur. Acute acalculous cholecystitis (ACC) is a rare complication of acute hepatitis B virus infection; only few cases are reported as ACC associated with acute hepatitis B virus infection. ACC cases are self-limiting, while other limited cases can progress to a gangrenous state, perforation, and even death. We present a 27-year-old female case diagnosed to have acute acalculous cholecystitis and associated with acute hepatitis B virus infection, and she recovered within one week of her presentation without complication or surgical intervention.

Can subacute thyroiditis be associated with acute hepatitis B virus infection?

2012 ◽  
Vol 124 (9-10) ◽  
pp. 348-349 ◽  
Author(s):  
Adnan Tas ◽  
Yavuz Beyazit ◽  
Sehmuz Olmez ◽  
Hacer Celik ◽  
Selma Aktas
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Acute Hepatitis ◽  
Subacute Thyroiditis ◽  
Hepatitis B Virus Infection ◽  
B Virus ◽  
Acute Hepatitis B
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