scholarly journals Emergence of Novel Human Norovirus GII.17 Strains Correlates With Changes in Blockade Antibody Epitopes

2017 ◽  
Vol 216 (10) ◽  
pp. 1227-1234 ◽  
Author(s):  
Lisa C Lindesmith ◽  
Jacob F Kocher ◽  
Eric F Donaldson ◽  
Kari Debbink ◽  
Michael L Mallory ◽  
...  
Keyword(s):  
Human Norovirus ◽  
Norovirus Gii ◽  
Antibody Epitopes

scholarly journals Identification of Human Norovirus GII.3 Blockade Antibody Epitopes

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 2058
Author(s):  
Yufang Yi ◽  
Shuxia Wang ◽  
Xiaoli Wang ◽  
Pei Xiong ◽  
Qingwei Liu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Acute Gastroenteritis ◽  
Pediatric Population ◽  
Blood Group Antigens ◽  
Human Norovirus ◽  
Chimeric Vlps ◽  
Virus Like Particle ◽  
Norovirus Gii ◽  
Vp1 Capsid Protein ◽  
Antibody Epitopes

Human noroviruses are a common pathogen causing acute gastroenteritis worldwide. Among all norovirus genotypes, GII.3 is particularly prevalent in the pediatric population. Here we report the identification of two distinct blockade antibody epitopes on the GII.3 capsid. We generated a panel of monoclonal antibodies (mAbs) from mice immunized with virus-like particle (VLP) of a GII.3 cluster 3 strain. Two of these mAbs, namely 8C7 and 8D1, specifically bound the parental GII.3 VLP but not VLPs of GII.4, GII.17, or GI.1. In addition, 8C7 and 8D1 efficiently blocked GII.3 VLP binding with its ligand, histo-blood group antigens (HBGA). These data demonstrate that 8C7 and 8D1 are GII.3-specific blockade antibodies. By using a series of chimeric VLPs, we mapped the epitopes of 8C7 and 8D1 to residues 385–400 and 401–420 of the VP1 capsid protein, respectively. These two blockade antibody epitopes are highly conserved among GII.3 cluster 3 strains. Structural modeling shows that the 8C7 epitope partially overlaps with the HBGA binding site (HBS) while the 8D1 epitope is spatially adjacent to HBS. These findings may enhance our understanding of the immunology and evolution of GII.3 noroviruses.

Experimental miniature piglet model for the infection of human norovirus GII

2017 ◽  
Vol 90 (4) ◽  
pp. 655-662 ◽  
Author(s):  
Dong Joo Seo ◽  
Day Jung ◽  
Soontag Jung ◽  
Seung-Kwon Ha ◽  
Sang-Do Ha ◽  
...  
Keyword(s):  
Human Norovirus ◽  
Norovirus Gii

scholarly journals Identification of a blockade epitope of human norovirus GII.17

2021 ◽  
pp. 1-17
Author(s):  
Yufang Yi ◽  
Xiaoli Wang ◽  
Shuxia Wang ◽  
Pei Xiong ◽  
Qingwei Liu ◽  
...  
Keyword(s):  
Human Norovirus ◽  
Norovirus Gii

scholarly journals Human norovirus GII.4(MI001) P dimer binds fucosylated and sialylated carbohydrates

Glycobiology ◽  
2017 ◽  
Vol 27 (11) ◽  
pp. 1027-1037 ◽  
Author(s):  
Henrik Wegener ◽  
Álvaro Mallagaray ◽  
Tobias Schöne ◽  
Thomas Peters ◽  
Julia Lockhauserbäumer ◽  
...  
Keyword(s):  
Human Norovirus ◽  
Norovirus Gii

scholarly journals Ìn situ inactivation of human norovirus GII.4 by cold plasma: Ethidium monoazide (EMA)-coupled RT-qPCR underestimates virus reduction and fecal material suppresses inactivation

2020 ◽  
Vol 85 ◽  
pp. 103307 ◽  
Author(s):  
Hamada A. Aboubakr ◽  
Fernando Sampedro Parra ◽  
James Collins ◽  
Peter Bruggeman ◽  
Sagar M. Goyal
Keyword(s):  
Cold Plasma ◽  
Fecal Material ◽  
Human Norovirus ◽  
Ethidium Monoazide ◽  
Norovirus Gii

scholarly journals Heterologous expression of human norovirus GII.4 VP1 leads to assembly of T=4 virus-like particles

2019 ◽  
Vol 168 ◽  
pp. 175-182 ◽  
Author(s):  
Jessica M. Devant ◽  
Götz Hofhaus ◽  
David Bhella ◽  
Grant S. Hansman
Keyword(s):  
Heterologous Expression ◽  
Human Norovirus ◽  
Virus Like Particles ◽  
Norovirus Gii

scholarly journals Quantification of Human Norovirus GII on Hands of Mothers with Children Under the Age of Five Years in Bagamoyo, Tanzania

2015 ◽  
Vol 93 (3) ◽  
pp. 478-484 ◽  
Author(s):  
Mia Catharine M. Mattioli ◽  
Alexandria B. Boehm ◽  
Mwifadhi Mrisho ◽  
Jennifer Davis
Keyword(s):  
Human Norovirus ◽  
Norovirus Gii

scholarly journals Conformational Occlusion of Blockade Antibody Epitopes, a Novel Mechanism of GII.4 Human Norovirus Immune Evasion

mSphere ◽  
2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Lisa C. Lindesmith ◽  
Michael L. Mallory ◽  
Kari Debbink ◽  
Eric F. Donaldson ◽  
Paul D. Brewer-Jensen ◽  
...  
Keyword(s):  
Immune Evasion ◽  
Particle Dynamics ◽  
Human Norovirus ◽  
Viral Pathogens ◽  
Virus Like Particles ◽  
Antibody Epitope ◽  
Binding Residues ◽  
Additional Support ◽  
Key Residues ◽  
Antibody Epitopes

In this study, we use norovirus virus-like particles to identify key residues of a conserved GII.4 blockade antibody epitope. Further, we identify an additional GII.4 blockade antibody epitope to be occluded, with antibody access governed by temperature and particle dynamics. These findings provide additional support for particle conformation-based presentation of binding residues mediated by a particle “breathing core.” Together, these data suggest that limiting antibody access to blockade antibody epitopes may be a frequent mechanism of immune evasion for GII.4 human noroviruses. Mapping blockade antibody epitopes, the interaction between adjacent epitopes on the particle, and the breathing core that mediates antibody access to epitopes provides greater mechanistic understanding of epitope camouflage strategies utilized by human viral pathogens to evade immunity.

scholarly journals Survival and inactivation of human norovirus GII.4 Sydney on commonly touched airplane cabin surfaces

2020 ◽  
Vol 7 (3) ◽  
pp. 574-586
Author(s):  
Dorra Djebbi-Simmons ◽  
◽  
Mohammed Alhejaili ◽  
Marlene Janes ◽  
Joan King ◽  
...  
Keyword(s):  
Human Norovirus ◽  
Norovirus Gii

scholarly journals Human norovirus GII.4 Hong Kong variant shares common ancestry with GII.4 Osaka and emerged in Thailand in 2016

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0256572
Author(s):  
Watchaporn Chuchaona ◽  
Jira Chansaenroj ◽  
Jiratchaya Puenpa ◽  
Sarawut Khongwichit ◽  
Sumeth Korkong ◽  
...  
Keyword(s):  
Hong Kong ◽  
Nucleotide Sequence ◽  
Acute Gastroenteritis ◽  
Age Groups ◽  
Epidemiological Surveillance ◽  
Common Ancestry ◽  
Amino Acid Residues ◽  
Human Norovirus ◽  
Susceptible Population ◽  
Norovirus Gii

Human norovirus is a leading cause of non-bacterial acute gastroenteritis, which affects all age groups and are found globally. Infections are highly contagious and often occur as outbreaks. Periodic emergence of new strains are not uncommon and novel variants are named after the place of first reported nucleotide sequence. Here, we identified human norovirus GII.4 Hong Kong variant in stool samples from Thai patients presented with acute gastroenteritis. Comparison of amino acid residues deduced from the viral nucleotide sequence with those of historical and contemporary norovirus GII.4 strains revealed notable differences, which mapped to the defined antigenic sites of the viral major capsid protein. Time-scaled phylogenetic analysis suggests that GII.4 Hong Kong shared common ancestry with GII.4 Osaka first reported in 2007, and more importantly, did not evolve from the now-prevalent GII.4 Sydney lineage. As circulation of norovirus minor variants can lead to eventual widespread transmission in susceptible population, this study underscores the potential emergence of the GII.4 Hong Kong variant, which warrants vigilant molecular epidemiological surveillance.

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